Abstract
Hydroxyapatite (HA) is a widely used calcium phosphate implant substitute and has dissolution property. Although HA has been shown a beneficial effect on osteoblast differentiation, the exact mechanism is still unclear. In the present study, we proposed that Ca2+ released from HA activated the expression bone associated proteins, OPN and BSP, mediated by L-type calcium channel and calcium/calmodulin-dependent protein kinase (CaMK) 2 which resulted into improved osteoblast differentiation. Results showed that HA elevated ALP expression as well as OPN and BSP expression in MC3T3-E1 cells. The result from western blot of CaMK2α indicated that HA released Ca2+ activated CaMK2 through L-type calcium channel. Furthermore, upregulation of OPN and BSP mRNA expression was significantly inhibited when blocking both the L-type calcium channel and CaMK2. These findings suggested that HA accelerated the osteoblast differentiation by releasing Ca2+.
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Acknowledgments
This study was supported by the grant of Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) No. R01-2007-000-10977-0 and Ministry of Science and Technology (Regenomics, M10528010004-06N2801-00410).
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Jung, GY., Park, YJ. & Han, JS. Effects of HA released calcium ion on osteoblast differentiation. J Mater Sci: Mater Med 21, 1649–1654 (2010). https://doi.org/10.1007/s10856-010-4011-y
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DOI: https://doi.org/10.1007/s10856-010-4011-y