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Development of in situ-forming hydrogels for hemorrhage control

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Abstract

We report the preparation of in situ-forming hydrogels, composed of oxidized dextran (Odex) and amine-containing polymers, for their potential use as a wound dressing to promote blood clotting. Dextran was oxidized by sodium periodate to introduce aldehyde groups to form hydrogels, upon mixing in solution with different polymers containing primary amine groups, including polyallylamine (PAA), oligochitosan and glycol chitosan. A series of experiments were conducted to identify the optimum gelation condition for the Odex-PAA system. The polymer concentration appeared to have a major effect on gelation time and the polymer weight ratio affected the resulting gel content and swelling. Other influencing factors included pH of the buffer used to dissolve each polymer, PAA molecular weight, and the type of individual material. The latter also contributed significantly to gel content and swelling. Thromboelastography was used to examine the effects of the in situ gelation on blood coagulation in vitro, where the Odex-PAA combination was found to be most pro-hemostatic, as indicated by a decrease in clotting time and an increase in clot strength. The results of this study demonstrated that in situ-forming hydrogels could promote clotting in vitro; however, further studies are required to determine if the same hydrogel formulations are effective in controlling hemorrhage in vivo.

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Acknowledgements

The authors are indebted to Mr. Clark Chen, Mr. Anup Tuladhar and Ms. Heather Wright for their technical assistance.

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  1. Defence Research and Development Canada – Toronto, 1133 Sheppard Avenue West, Toronto, ON, M3M 3B9, Canada

    Henry T. Peng & Pang N. Shek

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  1. Henry T. Peng
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Correspondence to Henry T. Peng.

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Peng, H.T., Shek, P.N. Development of in situ-forming hydrogels for hemorrhage control. J Mater Sci: Mater Med 20, 1753–1762 (2009). https://doi.org/10.1007/s10856-009-3721-5

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