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Quantification of bone mass gain in response to the application of biphasic bioceramics and platelet concentrate in critical-size bone defects

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Abstract

Biphasic bioceramics have been widely indicated for bone reconstruction; however, the real gain in bone mass due to the presence of such biomaterials has not been established yet nor the advantages of its association with platelet concentrate. This study aims at quantifying the volume of bone matrix, osteoblasts, osteocytes, blood vessels and adipose tissue after the application of a biphasic bioceramics composed of 65% hydroxyapatite and 35% β-tricalcium phosphate. Critical-size bone defects were produced in rabbit femora and reconstructed with bioceramics only, with bioceramics combined with platelet concentrate, with platelet concentrate alone, and with no treatment (blood clot). The quantitative evaluation was performed on histological sections using histomorphometry. Our data provide original evidence that consolidates the indication of bioceramics for clinical bone loss reconstruction. The application of biphasic bioceramics alone led to major bone mass gain and was followed by its association with platelet concentrate. On the other hand, platelet concentrate can contribute to the augmentation and maintenance of the adipose tissue, representing a new field for future applications in plastic surgery.

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Acknowledgment

This work was funded by EINCO Biomaterial Ltda (Belo Horizonte, Minas Gerais, Brazil).

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Authors and Affiliations

  1. Morphology Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Sonja Ellen Lobo, Germán Bohorquez Mahecha & Humberto José Alves

  2. Genetics Laboratory, Butantan Institute, Avenida Vital Brazil 1500, São Paulo, SP, CEP 05503-900, Brazil

    Sonja Ellen Lobo & Irina Kerkis

  3. Hospital São Bento Cardioclínica S/A and EINCO Biomaterial Ltd., Belo Horizonte, Minas Gerais, Brazil

    Francisco Henrique Lanna Wykrota

  4. Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Ana Carolina Marques Barbosa Oliveira

Authors
  1. Sonja Ellen Lobo
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  2. Francisco Henrique Lanna Wykrota
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  3. Ana Carolina Marques Barbosa Oliveira
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  4. Irina Kerkis
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  5. Germán Bohorquez Mahecha
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  6. Humberto José Alves
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Correspondence to Sonja Ellen Lobo.

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Lobo, S.E., Wykrota, F.H.L., Oliveira, A.C.M.B. et al. Quantification of bone mass gain in response to the application of biphasic bioceramics and platelet concentrate in critical-size bone defects. J Mater Sci: Mater Med 20, 1137–1147 (2009). https://doi.org/10.1007/s10856-008-3660-6

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  • DOI: https://doi.org/10.1007/s10856-008-3660-6

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