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Bone resembling apatite by amorphous-to-crystalline transition driven self-organisation

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Abstract

Calcium apatite is the main inorganic constituent of mammalian hard tissues such as bones and teeth. Its formation in vivo is likely to be preceded by a transient amorphous phase. If so, the amorphous-to-crystalline transition would have some crucial role in the biomineralisation process. To investigate this possibility, a two-step biomimetic experiment was designed. First, a stable amorphous calcium apatite precursor was synthesized in simulated body fluid (SBF) and was then transformed into a low crystalline apatite. X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, vacuum FTIR, inductively coupled plasma-atomic emission spectrometry (ICP-AES), scanning electron microscopy (SEM) and N2 adsorption measurements were used to characterise both the precursor and the apatite. The latter exhibits numerous bone-like features including lack of OH, nanometer size, low crystallinity, etc. An amorphous-to-crystalline transition driven self-organisation is observed. The amorphous precursor seems to be the essential step for the creation of bone resembling apatite.

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Acknowledgements

One of the authors (Ya. Pekounov) wishes to express his sincere gratitude to K. Chakarova for the IR technical assistance and to I. Dimov for his valuable help with the preparation of the manuscript.

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Authors and Affiliations

  1. Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria

    Yassen Pekounov

  2. Central Laboratory of Mineralogy and Crystallography, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria

    Ognyan E. Petrov

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  1. Yassen Pekounov
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  2. Ognyan E. Petrov
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Correspondence to Yassen Pekounov.

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Pekounov, Y., Petrov, O.E. Bone resembling apatite by amorphous-to-crystalline transition driven self-organisation. J Mater Sci: Mater Med 19, 753–759 (2008). https://doi.org/10.1007/s10856-007-3085-7

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  • DOI: https://doi.org/10.1007/s10856-007-3085-7

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