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Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes

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Abstract

A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of Trypanosoma cruzi onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m2. In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m2, around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point).

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Acknowledgments

We are grateful to CONICET, SeCyT, Universidad Nacional del Litoral, and Universidad Nacional de Córdoba (Argentina) for the financial supports. Also, we thank Wiener Laboratory (Rosario, Argentina) for the kind donation of protein Ag36.

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Authors and Affiliations

  1. INTEC (Universidad Nacional del Litoral and CONICET), Güemes 3450, Santa Fe, 3000, Argentina

    Verónica D. G. Gonzalez, Luis M. Gugliotta & Gregorio R. Meira

  2. INFIQC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Cordoba, 5000, Argentina

    Carla E. Giacomelli

Authors
  1. Verónica D. G. Gonzalez
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  2. Luis M. Gugliotta
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  3. Carla E. Giacomelli
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  4. Gregorio R. Meira
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Correspondence to Gregorio R. Meira.

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Gonzalez, V.D.G., Gugliotta, L.M., Giacomelli, C.E. et al. Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes. J Mater Sci: Mater Med 19, 789–795 (2008). https://doi.org/10.1007/s10856-006-0041-x

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  • DOI: https://doi.org/10.1007/s10856-006-0041-x

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