Skip to main content
SpringerLink
Log in

Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissue engineering approaches with adipose tissue derived stem cells

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

An Erratum to this article was published on 01 July 2006

Abstract

It is well accepted that natural tissue regeneration is unlikely to occur if the cells are not supplied with an extracellular matrix (ECM) substitute. With this goal, several different methodologies have been used to produce a variety of 3D scaffolds as artificial ECM substitutes suitable for bone and cartilage tissue engineering. Furthermore, osteochondral tissue engineering presents new challenges since the combination of scaffolding and co-culture requirements from both bone and cartilage applications is required in order to achieve a successful osteochondral construct.

In this paper, an innovative processing route based on a chitosan particles aggregation methodology for the production of cartilage and osteochondral tissue engineering scaffolds is reported. An extensive characterization is presented including a morphological evaluation using Micro-Computed Tomography (μCT) and 3D virtual models built with an image processing software. Mechanical and water uptake characterizations were also carried out, evidencing the potential of the developed scaffolds for the proposed applications. Cytotoxicity tests show that the developed chitosan particles agglomerated scaffolds do not exert toxic effects on cells. Furthermore, osteochondral bilayered scaffolds could also be developed. Preliminary seeding of mesenchymal stem cells isolated from human adipose tissue was performed aiming at developing solutions for chondrogenic and osteogenic differentiation for osteochondral tissue engineering applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. E. GOMES, A. S. RIBEIRO, P. B. MALAFAYA, R. L. REIS and A. M. CUNHA, Biomaterials 22 (2001) 883.

    Article  CAS  Google Scholar 

  2. Y. DENG, K. ZHAO, X.-F. ZHANG, P. HU and G.-Q. CHEN, ibid. 23 (2002) 4049.

    Article  CAS  Google Scholar 

  3. Q. HOU, D. W. GRIJPMA and J. FEIJEN, ibid. 24 (2003) 1937.

    Article  CAS  Google Scholar 

  4. H. S. YOO, E. A. LEE, J. J. YOON and T. G. PARK, ibid. 26 (2005) 1925.

    Article  CAS  Google Scholar 

  5. M. NOF and L. D. SHEA, J. Biomed. Mater. Res. 59 (2002) 349.

    Article  CAS  Google Scholar 

  6. M. E. GOMES, V. I. SIKAVITSAS, E. BEHRAVESH, R. L. REIS and A. G. MIKOS, J. Biomed. Mater. Res. 67A (2003) 87.

    Article  CAS  Google Scholar 

  7. S. V. MADIHALLY and H. W. T. MATTHEW, Biomaterials 20 (1999) 1133.

    Article  CAS  Google Scholar 

  8. P. B. MALAFAYA and R. L. REIS, in “Key Eng Mat” edited by B. Ben-Nissan, D. Sher and W. Walsh (Trans Tech Publications, Zurich 2003) p. 39.

  9. S. ZMORA, R. GLICKLIS and S. COHEN, Biomaterials 23 (2002) 4087.

    Article  CAS  Google Scholar 

  10. K. F. LEONG, C. M. CHEAH and C. K. CHUA, ibid. 24 (2003) 2363.

    Article  CAS  Google Scholar 

  11. I. ZEIN, D. W. HUTMACHER, K. C. TAN and S. H. TEOH, ibid. 23 (2002) 1169.

    Article  CAS  Google Scholar 

  12. E. SACHLOS, N. REIS, C. AINSLEY, B. DERBY and J. T. CZERNUSZKA, ibid. 24 (2003) 1487.

    Article  CAS  Google Scholar 

  13. M. BORDEN, S. F. EL-AMIN, M. ATTAWIA and C. T. LAURENCIN, ibid. 24 (2003) 597.

    Article  CAS  Google Scholar 

  14. M. BORDEN, M. ATTAWIA and C. T. LAURENCIN, J. Biomed. Mater. Res. 61 (2002) 421.

    Article  CAS  Google Scholar 

  15. M. BORDEN, M. ATTAWIA, Y. KHAN and C. T. LAURENCIN, Biomaterials 23 (2002) 551.

    Article  CAS  Google Scholar 

  16. J. E. DEVIN, M. A. ATTAWIA and C. T. LAURENCIN, J. Biomat. Sci. Polym. Ed. 7 (1996) 661.

    CAS  Google Scholar 

  17. D. H. R. KEMPEN, C. W. KIM, L. LU, W. J. A. DHERT, B. L. CURRIER and M. J. YASZEMSKI, Mater. Sci. Forum. 426–424 (2003) 3151.

    Google Scholar 

  18. T. A. HOLLAND, Y. TABATA and A. G. MIKOS, J. Control. Release. 91 (2003) 299.

    Article  CAS  Google Scholar 

  19. R. G. PAYNE, J. S. MCGONIGLE, M. J. YASZEMSKI, A. W. YASKO and A. G. MIKOS, Biomaterials 23 (2002) 4381.

    Article  CAS  Google Scholar 

  20. R. G. PAYNE, M. J. YASZEMSKI, A. W. YASKO and A. G. MIKOS, ibid. 23 (2002) 4359.

    Article  CAS  Google Scholar 

  21. E. L. HEDBERG, A. TANG, R. S. CROWTHER, D. H. CARNEY and A. G. MIKOS, J. Control. Release. 84 (2002) 137.

    Article  CAS  Google Scholar 

  22. T. M. MEESE, Y. HU, R. W. NOWAK and K. G. MARRA, J. Biomater. Sci. Polym. Ed. 13 (2002) 141.

    Article  CAS  Google Scholar 

  23. S. E. KIM, J. H. PARK, Y. W. CHO, H. CHUNG, S. Y. JEONG, E. B. LEE and I. C. KWON, J. Control. Release. 91 (2003) 365.

    Article  CAS  Google Scholar 

  24. A. PERETS, Y. BARUCH, F. WEISBUCH, G. SHOSHANY, G. NEUFELD and S. COHEN, J. Biomed. Mater. Res. 65A (2003) 489.

    Article  CAS  Google Scholar 

  25. C. T. HUNG, E. G. LIMA, R. L. MAUCK, E. TAKI, M. A. LEROUX, H. H. LU, R. G. STARK, X. E. GUO and G. A. ATESHIAN, J. Biomech. 36 (2003) 1853.

    Article  Google Scholar 

  26. J. K. SHERWOOD, S. L. RILEY, R. PALAZZOLO, S. C. BROWN, D. C. MONKHOUSE, M. COATES, L. G. GRIFFITH, L. K. LANDEEN and A. RATCLIFFE, Biomaterials 23 (2002) 4739.

    Article  CAS  Google Scholar 

  27. P. ANGELE, R. KUJAT, M. NERLICH, J. YOO, V. GOLDBERG and B. JOHNSTONE, Tissue. Eng. 5 (1999) 545.

    CAS  Google Scholar 

  28. J. S. TEMENOFF and A. G. MIKOS, Biomaterials 21 (2000) 431.

    Article  CAS  Google Scholar 

  29. R. K. SANDERS and J. R. CRIM, Seminars in Ultrasound, CT, and MRI 22 (2001) 352.

    Article  CAS  Google Scholar 

  30. J. A. BUCKWALTER, S. L. WOO, V. M. GOLDBERG, E. C. HADLEY, F. BOOTH, T. R. OEGEMA and D. R. EYRE, J. Bone and Joint Surg. Am. 10 (1993) 1533.

    Google Scholar 

  31. L. HANGODY, P. FECZKO, L. BARTHA, G. BODO and G. KISH, Clin. Orthop. Relat. R (2001) S328.

  32. J. GAO, J. E. DENNIS, L. A. SOLCHAGA, V. M. GOLDBERG and A. I. CAPLAN, Tissue Eng. 8 (2002) 827.

    Article  CAS  Google Scholar 

  33. T. TAGUCHI, Y. SAWABE, H. KOBAYASHI, Y. MORIYOSHI, K. KATAOKA and J. TANAKA, Mater. Sci. Eng. C 24 (2004) 881.

    Article  Google Scholar 

  34. J. P. SPALAZZI, K. L. MOFFAT, S. B. DOTY and H. H. LU, in “Proceedings of the Society for Biomaterials 30th Annual Meeting Transactions” (Memphis, 2005) p. 253.

  35. J. JIANG, C. T. HUNG, X. E. GUO, G. A. ATESHIAN and H. H. LU, in “Proceedings of the 7th World Biomaterials Congress” (Sydney, 2004) p. 7.

  36. R. A. SOUSA, R. L. REIS, A. M. CUNHA and M. J. BEVIS, J. Appl. Polym. Sci. 86 (2002) 2873.

    Article  CAS  Google Scholar 

  37. P. B. MALAFAYA and R. L. REIS, in “Proceedings of the 7th World Biomaterials Congress” (Sydney, 2004) p. 1285.

  38. P. B. MALAFAYA and R. L. REIS, in “Proceedings of 6th International Symposium on Frontiers in Biomedical Polymers-FBPS'05” (Granada, 2005) p. O19.

  39. P. A. ZUK, M. ZHU, P. ASHJIAN, D. A. DE UGARTE, J. I. HUANG, H. MIZUNO, Z. C. ALFONSO, J. K. FRASER, P. BENHAIM and M. H. HEDRICK, Mol. Biol. Cell 13 (2002) 4279.

    Article  CAS  Google Scholar 

  40. L. J. CURRIE, J. R. SHARPE and R. MARTIN, Plast. Reconstr. Surg. 108 (2001) 1713.

    Article  CAS  Google Scholar 

  41. H. REDL, in “Tissue Glues in Cosmetic Surgery” (Quality Medical Publishing Inc., St. Louis, 2004), p. 27.

  42. G. KAPLONYI, I. ZIMMERMAN, A. D. FRENYO, T. FARKAS and G. NEMES, Injury 19 (1988) 267.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. 3B's Research Group—Biomaterials, Biomimetics and Biodegradables, Dept. Polymer Eng., Univ. of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    P. B. Malafaya, A. J. Pedro, H. Redl & R. L. Reis

  2. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstr. 13, A-1200, Vienna, Austria

    A. Peterbauer

  3. Red Cross Transfusion Service for Upper Austria, Blumauerstr. 3-5, A-4020, Linz, Austria

    A. Peterbauer & C. Gabriel

Authors
  1. P. B. Malafaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. J. Pedro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Peterbauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Gabriel
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Redl
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. L. Reis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. B. Malafaya.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s10856-006-9231-9.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Malafaya, P.B., Pedro, A.J., Peterbauer, A. et al. Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissue engineering approaches with adipose tissue derived stem cells. J Mater Sci: Mater Med 16, 1077–1085 (2005). https://doi.org/10.1007/s10856-005-4709-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-005-4709-4

Keywords

Search

Navigation