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Silk microfiber-reinforced silk composite scaffolds: fabrication, mechanical properties, and cytocompatibility

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Abstract

Mechanical reinforcement of silk membranes by embedding silk microfibers was studied. Silk microfibers of 10–600 μm long were prepared by hydrolyzing degummed silk fibers in alkali solution. The silk microfibers were mixed with silk fibroin solution (continuous phase) at various ratios to fabricate silk microfiber-reinforced composite scaffolds (SMCSs). The morphology, mechanical properties, structural characteristics, biodegradation, and cytotoxicity of the composites were investigated. Silk microfiber-reinforced membranes with 1 wt% of microfibers displayed the most homogeneous distribution of microfibers in the membrane matrix. The tensile strength and elongation at break were 10.5 ± 2.7 MPa and 56.7 ± 7.8 %, respectively, comparable to human meniscus tissue. The presence of silk microfibers did not significantly impact the secondary structure and crystallization of SMCSs. Proteolytic degradation in vitro using protease XIV showed that the 1 % silk microfiber-reinforced membranes lost 90 % weight after 5 days, a longer time frame than plain silk membrane controls. The viability of human fibroblasts (Hs 865.Sk) on the SMCSs demonstrated no cytotoxicity. SMCSs may be useful as biomaterial systems as tissue substitutes where mechanical strength is critical for functional performance.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (NSFC, 51273138) and the National Institutes of Health (P41 EB002520). We would like to thank the support of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University (LK1421) and Natural Science Foundation of Jiangsu Province (BK20150372).

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    Authors and Affiliations

    1. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, People’s Republic of China

      Gang Li, Fei Li, Zhaozhu Zheng, Tingting Luo, Jian Liu, Jianbing Wu, Xiaoqin Wang & David L. Kaplan

    2. Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA, 02155, USA

      Xiaoqin Wang & David L. Kaplan

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    1. Gang Li
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    2. Fei Li
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    3. Zhaozhu Zheng
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    5. Jian Liu
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    8. David L. Kaplan
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    Correspondence to Xiaoqin Wang or David L. Kaplan.

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    Gang Li and Fei Li have contributed to this manuscript equally.

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    Li, G., Li, F., Zheng, Z. et al. Silk microfiber-reinforced silk composite scaffolds: fabrication, mechanical properties, and cytocompatibility. J Mater Sci 51, 3025–3035 (2016). https://doi.org/10.1007/s10853-015-9613-9

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    • DOI: https://doi.org/10.1007/s10853-015-9613-9

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