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A casein-polysaccharide hybrid hydrogel cross-linked by transglutaminase for drug delivery

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Abstract

A protein-polysaccharide hydrogel was reported as a biocompatible, biodegradable, and non-toxic material that had biomedical applications such as drug delivery. The hydrogel, composed of 10% casein and 1% konjac glucomannan (KGM), was formed with 0.4 wt% transglutaminase (MTG) as the cross-linker. The physicochemical properties of the protein-polysaccharide hydrogel were investigated by SEM observation, FT-IR analysis, swelling ratio test, and stability test. The results of the stability test proved that the hydrogel with KGM had an obviously improved stability. Its degradation rate also decreased from 100% to less than 60% compared with the hydrogel without KGM at the end of the test. The results of the swelling ratio test demonstrated that the addition of KGM restricted the mobility of the chains, and decreased the swelling ratio of the hydrogel. The results of the FT-IR revealed hydrogen bond interactions during the gelation process upon the addition of KGM. To investigate in vitro release behavior, docetaxel was chosen as a model drug incorporated into the casein/KGM hydrogels. The hydrogel with 1% KGM exhibited a good drug release behavior.

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Acknowledgements

The authors acknowledge the financial supports of the Natural Science Foundation of China (Grant Nos. 20806057 and 31071509), and of the Ministry of Education (Grant Nos. 200800561004 and NCET-08-0386).

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Authors and Affiliations

  1. State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Wenwen Yin, Rongxin Su, Wei Qi & Zhimin He

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  1. Wenwen Yin
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  2. Rongxin Su
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  3. Wei Qi
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  4. Zhimin He
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Correspondence to Rongxin Su.

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Yin, W., Su, R., Qi, W. et al. A casein-polysaccharide hybrid hydrogel cross-linked by transglutaminase for drug delivery. J Mater Sci 47, 2045–2055 (2012). https://doi.org/10.1007/s10853-011-6005-7

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  • DOI: https://doi.org/10.1007/s10853-011-6005-7

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