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The effects of AdipoRon on cytochrome P450-related gene expression, acute steroidogenic regulatory protein, and structure of ovary in polycystic ovary syndrome model

  • Reproductive physiology and disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

One of the most common causes of infertility in adult women is polycystic ovary syndrome (PCOS) which has been identified with symptoms such as chronic hyperandrogenism, anovulation, and polycystic ovaries. Adiponectin modulates steroidogenesis and the expression of ovulation-related genes. Herein, we assessed the effect of AdipoRon (adiponectin agonist) in the PCOS model mice.

Methods

The PCOS model was induced with letrozole in the adult female mice and the animals received intraperitoneal injection of AdipoRon (5 mg/kg) for 10 days. Expression of CYP11A, CYP17A, and CYP19A genes, StAR protein, and histomorphology of the ovary were evaluated using real-time RT-PCR, western blotting, and histochemistry methods, respectively.

Results

Although administration of letrozole caused an increase in the expression of CYP11A, CYP17A, and StAR and a decrease in the CYP19A1 expression, injection of AdipoRon reversed these changes. Moreover, AdipoRon treatment resulted in an improvement of folliculogenesis and a reduction of cysts compared to the letrozole-treated mice.

Conclusion

It is likely that AdipoRon has protective effects on the PCOS through modulation of cytochrome P450-related genes and steroidogenesis but needs further study to be sure.

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Data availability

The data presented in this study are available on request from the corresponding author.

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Funding

This study was supported by the Hamadan University of Medical Sciences (9906254309).

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Pegah Hazrati, Mahdi Ramezani, Sara Soleimani Asl & Tayebe Artimani

  2. Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran

    Mahdi Ramezani, Sara Soleimani Asl & Tayebe Artimani

  3. Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

    Davoud Ahmadimoghaddam

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  1. Pegah Hazrati
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  2. Mahdi Ramezani
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Correspondence to Tayebe Artimani.

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Hazrati, P., Ramezani, M., Ahmadimoghaddam, D. et al. The effects of AdipoRon on cytochrome P450-related gene expression, acute steroidogenic regulatory protein, and structure of ovary in polycystic ovary syndrome model. J Assist Reprod Genet 40, 2453–2461 (2023). https://doi.org/10.1007/s10815-023-02900-1

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  • DOI: https://doi.org/10.1007/s10815-023-02900-1

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