Abstract
Purpose
To explore the whole-chromosome status, origins, and mechanisms of chromosomal abnormalities in good-quality cleavage embryos using multiple annealing and looping-based amplification cycle (MALBAC) sequencing.
Methods
The embryos studied came from7 patients (maternal aged 26–35) who had healthy birth from the same IVF cycles. These 21 frozen day 3 good-quality embryos were thawed and disaggregated into individual blastomere. Each blastomere was collected and analyzed by MALBAC sequencing.
Results
Conclusive results were obtained from a high percentage of blastomeres (95.3%). A total of 46.6% of blastomeres were diploid, 53.4% were abnormal, and 28.0% had complex aneuploidy. Out of 21 embryos, 3 (14.3%) were normal and 18 (85.7%) were mosaics, showing the occurrence of mitotic errors; aneuploidy was confirmed in all cells of 4 of the 18 embryos, which showed the coexistence of meiotic errors. Conclusive results were obtained from all blastomeres of 15 embryos (71.4%, 15/21), which enabled us to reconstruct the cell lineage on the basis of the chromosomal content of the blastomeres in each division. There were 9 mitotic errors (8.7%, 9/103): nondisjunction accounted for 88.9% (8/9), and endoreplication accounted for 11.1% (1/9).
Conclusions
In good-quality embryos, there was a high rate and diverse array of chromosomal abnormalities. Morphological evaluation does not appear to assist in the reduction in meiotic errors from parental origins. Mitotic errors were common, and nondisjunction was found to be the main mechanism causing malsegregation during the cleavage divisions.
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Funding
Supported by the Natural Science Foundation of Guangxi Province (grant number 2017GXNSFAA198149, 2017GXNSFAA198163) and the Major Science and Technology of Nanning (grant no. 20153011, 20153124, 20163138)
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The study was approved by the Institutional Review Board of the 3rd Affiliated Hospital of Guangxi Medical University. We obtained signed consent from all of the donors before the treatment.
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Shi, Q., Qiu, Y., Xu, C. et al. Next-generation sequencing analysis of each blastomere in good-quality embryos: insights into the origins and mechanisms of embryonic aneuploidy in cleavage-stage embryos. J Assist Reprod Genet 37, 1711–1718 (2020). https://doi.org/10.1007/s10815-020-01803-9
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DOI: https://doi.org/10.1007/s10815-020-01803-9