Abstract
Purpose
To explore whether it is possible to predict the number and quality of the embryo using a few particular hTERT SNPs.
Methods
We included 997 Han Chinese women who were genetically unrelated and underwent assisted reproduction using IVF from September 2014 to December 2015. DNA was genotyped by using TaqMan real-time quantitative PCR.
Results
Among the 997 patients, individuals with the CC genotype of rs2075786 had a significantly lower number of good-quality embryos than those with the TT+TC genotypes. Compared with the CT+CC genotype carriers, patients carrying the TT genotype of rs2853677 had a significantly lower number of oocytes retrieved, mature oocytes and available embryos. Among the 750 patients aged ≤ 35 years, individuals with the AA+AG genotypes of rs2853691 had a significantly higher number of good-quality embryos than those with the GG genotype. The haplotype analysis showed that the TTTG (rs2853672/rs2853669/rs2735940/rs2736108) haplotype was more likely to lead to more than three good-quality embryos in patients aged ≤ 35 years.
Conclusions
Our study suggests that the hTERT SNP is associated with IVF outcomes.
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Acknowledgements
The authors would like to thank all of the research workers who contributed to this study. We thank Professor Yiming Wang, a senior geneticist, for the genetics advice and Professor Caixia Li, an advanced statistician, for the statistical support.
Funding
Merck Serono China Research Fund for Fertility Experts; Guangdong Medical Science and Technology Research Fund (Grant No. A2015143); Guangdong Provincial Natural Science Foundation (Grant No. 2015A030313086); Special Fund for Public Welfare Research and Capacity Building in Guangdong Province (social development areas) (Grant No. 2014A020213014). These funding sources provided financial support for the conduct of the research.
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All authors contributed significantly to this work. Wenjun Wang designed the research study and wrote the paper; Kailing Dai, Hongmei Xu and Xiaomiao Zhao performed the experiments, analysed the data and wrote the manuscript; Nengyong Ouyang and Ping Yuan performed the experiments; Liangan Wang followed up the patients and collected the data; Ying Li collected the data and prepared figures and tables. All authors reviewed the manuscript. In addition, all authors approved the final draft.
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The project was approved by the reproductive ethics committee of the Sun Yat-Sen Memorial Hospital and has been registered in the Chinese Clinical Trial Registry under registration number ChiCTR-OCC-14005259.
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The authors declare that they have no conflicts of interest.
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Dai, K., Xu, H., Ouyang, N. et al. Correlation of human telomerase reverse transcriptase single nucleotide polymorphisms with in vitro fertilisation outcomes. J Assist Reprod Genet 36, 517–527 (2019). https://doi.org/10.1007/s10815-018-1379-y
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DOI: https://doi.org/10.1007/s10815-018-1379-y