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Superoxide dismutase in polycystic ovary syndrome patients undergoing intracytoplasmic sperm injection

  • Gonadal Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Polycystic ovary syndrome (PCOS) is associated with oxidative stress (OS) and serum superoxide dismutase (SOD) activity has been reported with mixed results. The objective of this study was to examine the activity of SOD both in the serum and FF from women with PCOS undergoing ICSI, as well as the expression of Cu/Zn-SOD mRNA in the cells recovered from the FF.

Methods

Forty women undergoing an ICSI trial were divided into: group I, included 20 PCOS cases, group II included 20 age-matched controls with tubal factor infertility. Both groups were similarly stimulated. A total of 204 metaphase II (MII) oocytes were aspirated; (108) from PCOS, and (96) from the control group. SOD activities in the serum and FF, as well as Cu/Zn-SOD (SOD1) mRNAs in follicular fluid (FF) cells were analyzed.

Results

There was a statistically highly significant decrease (p < 0.001) both in the mean serum SOD (45.56 ± 18.06) and FF SOD activity (42.49 ± 11.46) in PCOS than the control group (77.38 ± 7.82), (74.37 ± 6.15) respectively. The mean relative levels of Cu, Zn SOD mRNAs was significantly lower (p < 0.001) in cells isolated from the FF in PCOS (0.36 ± 0.14) than the control group (0.81 ± 0.15). SOD activity in FF had no effects on fertilization rate (p > 0.05), or embryo quality after intracytoplasmic sperm injection (ICSI).

Conclusion

Although decreased SOD activity in FF has no effect on fertilization rate and/or embryo quality, serum SOD activity could be a clinical parameter for determining systemic oxidative stress in PCOS.

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Authors and Affiliations

  1. Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Amal K. Seleem & Dalia Shaalan

  2. Department of Obstetrics and Gynecology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Abdel Aziz El Refaeey & Ahmed Badawy

  3. Leeds Institute of Rheumatic and Musculoskeletal Medicine (LIRMM), St James’s University Hospital, West Yorkshire, UK

    Yasser Sherbiny

  4. Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Yasser Sherbiny

Authors
  1. Amal K. Seleem
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  2. Abdel Aziz El Refaeey
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  3. Dalia Shaalan
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  4. Yasser Sherbiny
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  5. Ahmed Badawy
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Corresponding author

Correspondence to Ahmed Badawy.

Additional information

Capsule Serum superoxide dismutase (SOD) activity could be a clinical parameter for determining systemic oxidative stress in PCOS.

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Seleem, A.K., El Refaeey, A.A., Shaalan, D. et al. Superoxide dismutase in polycystic ovary syndrome patients undergoing intracytoplasmic sperm injection. J Assist Reprod Genet 31, 499–504 (2014). https://doi.org/10.1007/s10815-014-0190-7

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  • DOI: https://doi.org/10.1007/s10815-014-0190-7

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