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Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

  • ASSISTED REPRODUCTION TECHNOLOGIES
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

To compare the dynamics of early development between embryos cultured in single and sequential media.

Design

Randomized, comparative study.

Setting

Private IVF centre.

Patients

A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer.

Intervention

Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator.

Main outcome measures

Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates.

Results

Embryos cultured in single media were advanced from the first mitosis cycle and reached 2- to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc2 = t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media.

Conclusions

Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.

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Authors and Affiliations

  1. Fulya IVF Clinic, Bahceci Health Group, Hakki Yeten cd 11, Terrace Fulya, 34365, Istanbul, Turkey

    Haydar Nadir Ciray, Turan Aksoy, Cihan Goktas, Bilgen Ozturk & Mustafa Bahceci

Authors
  1. Haydar Nadir Ciray
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  2. Turan Aksoy
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  3. Cihan Goktas
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  4. Bilgen Ozturk
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  5. Mustafa Bahceci
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Corresponding author

Correspondence to Haydar Nadir Ciray.

Additional information

Capsule

Sibling oocytes were cultured until day 6 in single and sequential media in a time-lapse incubator. Morphokinetics of initial embryo development varied however overall clinical and embryological parameters remained similar.

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Ciray, H.N., Aksoy, T., Goktas, C. et al. Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study. J Assist Reprod Genet 29, 891–900 (2012). https://doi.org/10.1007/s10815-012-9818-7

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  • DOI: https://doi.org/10.1007/s10815-012-9818-7

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