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Complete organellar genomes and molecular phylogeny of Hypnea cervicornis (Gigartinales, Florideophyceae) from China

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Abstract

Hypnea cervicornis J. Agardh (Gigartinales, Florideophyceae) is a commercially important carrageenan producing seaweed. Currently, there are no organellar genomes of Hypnea species available in public databases. Here, we report the complete organellar genomes of H. cervicornis using next-generation sequencing technology. The mitochondrial genome has a circular mapping organization with a total length of 25,060 bp and consists of 50 genes (24 protein-coding, 2 rRNA, and 24 tRNA). The plastid genome is also a circular molecule and is 176,446 bp in length and includes 230 genes (194 protein-coding, 3 rRNA, 30 tRNA, 1 tmRNA and 2 misc_RNA). Colinear analysis show that the organellar genomes in the Gigartinales are conserved, except for the inversion of two genes (trnY and trnR) in the mitochondrial genome and a 12.5-kb rearrangement in the plastid genome. One stem-loop structure at the intergenic regions between trnS2 and trnA, plus one short hairpin structure between cob and trnL2 are detected in the mitochondrial genome of H. cervicornis. The Ka/Ks analysis reveal that values for most of the protein-coding genes in organellar genomes of H. cervicornis are below one, reflecting the importance of those genes. Phylogenetic relationships based on shared protein-coding genes from the organellar genomes of Rhodophyta are also examined.

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Funding

This work was supported by the Natural Science Foundation of Shandong Province (Grant No. ZR2019MC049), the National Natural Science Foundation of China (Grant No. 31402300), and Agricultural Seed Project of Shandong Province and TaiShan industrial Experts Program.

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  1. School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), 3501 Daxue Road, Jinan, 250353, Shandong Province, China

    Yu Xia, Pengjun Liu, Xiaoquan Liu, Jing Zhang, Xinfeng Tan & Yue Hu

  2. College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, Shandong Province, China

    Xuli Jia, Yuemei Jin & Tao Liu

  3. State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Sciences, Xiamen University, 422 Siming South Road, Xiamen, 361005, Fujian Province, China

    Tao Liu

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  1. Yu Xia
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Xia, Y., Liu, P., Liu, X. et al. Complete organellar genomes and molecular phylogeny of Hypnea cervicornis (Gigartinales, Florideophyceae) from China. J Appl Phycol 34, 2705–2717 (2022). https://doi.org/10.1007/s10811-022-02801-3

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