Abstract
Phlorotannins are polyphenolic compounds in brown algae and can be used as a natural UV filter in sunscreen formulations. The aim of the present study was to characterize the biological activities of phlorotannins-rich fractions from the brown alga Polycladia myrica and to validate the protective effect as well as stability of cream formulation with phlorotannins against UVR. With respect to antioxidant properties, the ethyl acetate fraction (EF) possessed the highest DPPH radical scavenging (65 ± 0.2%), and the highest antioxidant activity (11.2 ± 0.2 μg ASA mg−1). The EF was active against Gram-positive bacteria with EF effectively reducing UVB-induced cytotoxicity in HaCaT keratinocytes. The cream formulation with 5% EF revealed a high sun protective factor (31.79 ± 4.73), UVA/PF (24.67 ± 4.03), critical wavelength (383.2 ± 0.1 nm), and UVA/UVB ratio (0.98 ± 0.01). The cream formulation was completely homogeneous and had a pH close to human skin pH. The cream was stable in the cooling-heating cycle and the DPPH scavenging activity of the cream was not altered for 30 days of storage at temperatures of 4–40 °C. These findings are promising for the use of brown alga P. myrica extract as a valuable source of sunscreen protective substance for pharmaceutical and cosmetic applications.
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References
Afaq F, Adhami VM, Ahmad N, Mukhtar H (2002) Botanical antioxidants for chemoprevention of photocarcinogenesis. Front Biosci 7:784–792
Athikomkulchai S, Tunit P, Tadtong S, Jantrawut P, Sommano SR, Chittasupho C (2021) Moringa oleifera seed oil formulation physical stability and chemical constituents for enhancing skin hydration and antioxidant activity. Cosmetics 8:2
Badan Standardisasi Nasional (1996) Sediaan Tabir Surya. In: Dewan Standardisasi Nasional (SNl 16–4399–1996) pp 1–3
Bedoux G, Hardouin K, Burlot AS, Bourgougnon N (2014) Bioactive components from seaweeds: cosmetic applications and future development. Adv Bot Res 71:345–378
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76
Berthon J-Y, Nachat-Kappes R, Bey M, Cadoret J-P, Renimel I, Filaire E (2017) Marine algae as attractive source to skin care. Free Radical Res 51:555–567
Buranasukhon W, Athikomkulchai S, Tadtong S, Chittasupho C (2017) Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract. Pharm Biol 55:1767–1774
Chakraborty K, Praveen NK, Vijayan KK, Rao GS (2013) Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar. Asian Pac J Trop Biomed 3:8–16
Chan EWC, Lim YY, Omar M (2007) Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chem 104:1586–1593
Chen M, Tang R, Fu G, Xu B, Zhu P, Qiao S, Chen X, Xu B, Qin Y, Lu C, Hang B (2013) Association of exposure to phenols and idiopathic male infertility. J Hazard Mat 250:115–121
Chen MX, Alexander KS, Baki G (2016) Formulation and evaluation of antibacterial creams and gels containing metal ions for topical application. J Pharm 6:53–63
Cockell CS, Knowland J (1999) Ultraviolet radiation screening compounds. Biol Rev 74:311–345
Cotas J, Leandro A, Monteiro P, Pacheco D, Figueirinha A, Gonçalves AM, da Silva GJ, Pereira L (2020) Seaweed phenolics: from extraction to applications. Mar Drugs 18:384
de Araujo JM, Tappin MRR, da Rocha FR, Lopes-Filho EAP, Salgueiro F, De Paula JC (2018) Chemodiversity of the brown algae Canistrocarpus cervicornis (Dictyotaceae, Phaeophyceae) in tropical and subtropical populations along the southwestern Atlantic coast of Brazil. J Appl Phycol 30:611–618
de Lima Cherubim DJ, Buzanello Martins CV, Oliveira Fariña L, da Silva de Lucca RA (2020) Polyphenols as natural antioxidants in cosmetics applications. J Cosmet Dermatol 19:33–37
Dianursanti, Prakasa M, Nugroho P (2020) The effect of adding microalgae extract Spirulina platensis containing flavonoid in the formation of Sunscreen towards cream stability and SPF values. AIP Conference Proceedings 2255:040022
Diffey B, Robson J (1989) A new substrate to measure sunscreen protection factors throughout the ultraviolet spectrum. J Soc Cosmet Chem 40:127–133
Diffey BL, Tanner PR, Matts PJ, Nash JF (2000) In vitro assessment of the broad-spectrum ultraviolet protection of sunscreen products. J Am Acad Dermatol 43:1024–1035
Deepika C (2018) FTIR, SEM, EDS and GCMS metabolite profiling of macroalgae–Sargassum wightii. Int Res J Eng Technol 6:6791–6797
Dolorosa M, Purwaningsih S, Anwar E (2020) Utilization of Kappaphycus alvarezii and Sargassum plagyophyllum from Banten as cosmetic creams. IOP Conf Ser: Earth Environ Sci 404:012008
El-Sheekh MM, Mousa ASH, Farghl AA (2020) Antibacterial efficacy and phytochemical characterization of some marine brown algal extracts from the red sea, Egypt. Rom Biotech Lett 25:1160–1169
El Shoubaky GA, Salem EA (2014) Terpenes and sterols composition of marine brown algae Padina pavonica (Dictyotales) and Hormophysa triquetra (Fucales). Int J Pharm Phytochem Res 6:894–900
Etemadian Y, Shabanpour B, Ramzanpour Z, Shaviklo AR, Kordjazi M (2018) Nutritional and functional properties of two dried brown seaweeds Sirophysalis trinodis and Polycladia myrica. J Aquat Food Prod Technol 27:219–235
FDA (2011) Labeling and effectiveness testing; sunscreen drug products for over-the-counter human use. Food and Drug Administration, Final rule Federal register 76:35620
Fernando IPS, Dias MKHM, Madusanka DMD, Han EJ, Kim MJ, Jeon Y-J, Ahn G (2020a) Fucoidan refined by Sargassum confusum indicate protective effects suppressing photo-oxidative stress and skin barrier perturbation in UVB-induced human keratinocytes. Int J Biol Macromol 164:149–161
Fernando IPS, Dias MKHM, Madusanka DMD, Han EJ, Kim MJ, Jeon Y-J, Ahn G (2020b) Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects. Int J Biol Macromol 163:26–35
Ferrero L, Pissavini M, Dehais A, Marguerie S, Zastrow L (2007) Importance of substrate roughness for in vitro sun protection assessment. Int J Cosmet Sci 29:59–59
Fu L, Xu B-T, Xu X-R, Gan R-Y, Zhang Y, Xia E-Q, Li H-B (2011) Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem 129:345–350
Fussell JC, Kelly FJ (2020) Oxidative contribution of air pollution to extrinsic skin ageing. Free Radicals Biol Med 151:111
Gall EA, Lelchat F, Hupel M, Jégou C, Stiger-Pouvreau V (2015) Extraction and purification of phlorotannins from brown algae. In: Stengel DB, Connan S (eds) Natural products from marine algae. Method and protocols. Springer, Heidelberg, pp 131–143
Ghazipura M, McGowan R, Arslan A, Hossain T (2017) Exposure to benzophenone-3 and reproductive toxicity: a systematic review of human and animal studies. Reprod Toxicol 73:175–183
Giokas DL, Salvador A, Chisvert A (2007) UV filters: from sunscreens to human body and the environment. Trends Analyt Chem 26:360–374
González S, Fernández-Lorente M, Gilaberte-Calzada Y (2008) The latest on skin photoprotection. Clin Dermatol 26:614–26
Grice EA, Kong HH, Conlan S, Deming CB, Davis J, Young AC, Comparative Sequencing Program NISC, Bouffard GG, Blakesley RW, Murray PR, Green ED (2009) Topographical and temporal diversity of the human skin microbiome. Science 324:1190–1192
Guinea M, Franco V, Araujo-Bazán L, Rodríguez-Martín I, González S (2012) In vivo UVB-photoprotective activity of extracts from commercial marine macroalgae. Food Chem Toxicol 50:1109–1117
Haisma MS, Schuttelaar ML (2017) Contact urticaria caused by the ultraviolet absorber octocrylene in sunscreens. Contact Dermatitis 77:254–256
Hanson KM, Gratton E, Bardeen CJ (2006) Sunscreen enhancement of UV-induced reactive oxygen species in the skin. Free Radical Biol Med 41:1205–1212
Heo SJ, Ko SC, Cha SH, Kang DH, Park HS, Choi YU, Kim D, Jung WK, Jeon YJ (2009) Effect of phlorotannins isolated from Ecklonia cava on melanogenesis and their protective effect against photo-oxidative stress induced by UV-B radiation. Toxicol in Vitro 23:1123–1130
Heo S, Hwang HS, Jeong Y, Na K (2018) Skin protection efficacy from UV irradiation and skin penetration property of polysaccharide-benzophenone conjugates as a sunscreen agent. Carbohydr Polym 195:534–541
Herbert A, Martin M, Gilbert S (eds) (1988) Pharmaceutical emulsions, pharmaceutical dosage forms: disperse system. Marcel Dekker, New York, pp 285–288
Hojerová J, Medovcíková A, Mikula M (2011) Photoprotective efficacy and photostability of fifteen sunscreen products having the same label SPF subjected to natural sunlight. Int J Pharm 408:27–38
Jesumani V, Du H, Pei P, Aslam M, Huang N (2020) Comparative study on skin protection activity of polyphenol-rich extract and polysaccharide-rich extract from Sargassum vachellianum. PLoS One 15:e0227308
Joensen UN, Jørgensen N, Thyssen JP, Petersen JH, Szecsi PB, Stender S, Andersson AM, Skakkebæk NE, Frederiksen H (2017) Exposure to phenols, parabens and UV filters: Associations with loss-of-function mutations in the filaggrin gene in men from the general population. Environ Int 105:105–111
Kang KA, Lee KH, Chae S, Zhang R, Jung MS, Ham YM, Baik JS, Lee NH, Hyun JW (2006) Cytoprotective effect of phloroglucinol on oxidative stress induced cell damage via catalase activation. J Cell Biochem 97:609–620
Karthik R, Manigandan V, Sheeba R, Saravanan R, Rajesh PR (2016) Structural characterization and comparative biomedical properties of phloroglucinol from Indian brown seaweeds. J Appl Phycol 28:3561–3573
Karting T (1991) Still. F and Reinthaler. F, antimicrobial activity of the essential oil of young pine shoots. J Ethnopharmacol 35:155–157
Kokabi M, Yousefzadi M (2015) Checklist of the marine macroalgae of Iran. Bot Mar 58:307–320
Krause M, Klit A, Blomberg Jensen M, Søeborg T, Frederiksen H, Schlumpf M, Lichtensteiger W, Skakkebaek NE, Drzewiecki KT (2012) Sunscreens: are they beneficial for health? An overview of endocrine disrupting properties of UV-filters. Int J Androl 35:424–436
Kumar Santhanam R, Akhtar MT, Ahmad S, Abas F, Ismail IS, Rukayadi Y, Shaari K (2017) Utilization of the ethyl acetate fraction of Zanthoxylum rhetsa bark extract as an active ingredient in natural sunscreen formulations. Ind Crops Prod 96:165–172
Lautenschlager S, Wulf HC, Pittelkow MR (2007) Photoprotection. Lancet 370:528–537
Le Lann K, Surget G, Couteau C, Coiffard L, Cérantola S, Gaillard F, Larnicol M, Zubia M, Guérard F, Poupart N, Stiger-Pouvreau V (2016) Sunscreen, antioxidant, and bactericide capacities of phlorotannins from the brown macroalga Halidrys siliquosa. J Appl Phycol 28:3547–3559
Lee JH, Eom SH, Lee EH, Jung YJ, Kim HJ, Jo MR, Son KT, Lee HJ, Kim JH, Lee MS, Kim YM (2014) In vitro antibacterial and synergistic effect of phlorotannins isolated from edible brown seaweed Eisenia bicyclis against acne-related bacteria. Algae 29:47–55
Li J, Wang G, Gao C, Lv X, Wang Z, Liu H (2013) Deoxy-liquefaction of Laminaria japonica to high-quality liquid oil over metal modified ZSM-5 catalysts. Energy Fuels 27:5207–5214
Li J, Wang G, Chen M, Li J, Yang Y, Zhu Q, Jiang X, Wang Z, Liu H (2014) Deoxy-liquefaction of three different species of macroalgae to high-quality liquid oil. Bioresour Technol 169:110–118
Li Y, Fu X, Duan D, Liu X, Xu J, Gao X (2017) Extraction and identification of phlorotannins from the brown alga, Sargassum fusiforme (Harvey) Setchell. Mar Drugs 15:49
Liu N, Fu X, Duan D, Xu J, Gao X, Zhao L (2018) Evaluation of bioactivity of phenolic compounds from the brown seaweed of Sargassum fusiforme and development of their stable emulsion. J Appl Phycol 30:1955–1970
Lopes G, Sousa C, Silva LR, Pinto E, Andrade PB, Bernardo J, Mouga T, Valentão P (2012) Can phlorotannins purified extracts constitute a novel pharmacological alternative for microbial infections with associated inflammatory conditions? PloS One 7:e31145
Luthfiyana N, Hidayat T, Nurilmala M, Anwar E (2019) Utilization of seaweed porridge Sargassum sp and Eucheuma cottonii as cosmetic in protecting skin. IOP Conf Ser: Earth Environ Sci 278:012055
Maciel OMC, Tavares RSN, Caluz DRE, Gaspar LR, Debonsi HM (2018) Photoprotective potential of metabolites isolated from algae-associated fungi Annulohypoxylon stygium. J Photochem Photobiol B 178:316–322
Madivoli ES, Maina EG, Kairigo PK, Murigi MK, Ogilo JK, Nyangau JO, Kimani PK, Kipyegon C (2018) In vitro antioxidant and antimicrobial activity of Prunus africana (Hook. f.) Kalkman (bark extracts) and Harrisonia abyssinica Oliv. extracts (bark extracts): A comparative study. J Med Plant Econ Devel 2:1–9
Manandhar B, Paudel P, Seong SH, Jung HA, Choi JS (2019) Characterizing eckol as a therapeutic aid: A systematic review. Mar Drugs 17:361
McKenzie RL, Björn LO, Bais A, Ilyasd M (2003) Changes in biologically active ultraviolet radiation reaching the Earth’s surface. Photochem Photobiol Sci 2:5–15
Misonou T, Saitoh J, Oshiba S, Tokitomo Y, Maegawa M, Inoue Y, Hori H, Sakurai T (2003) UV-absorbing substance in the red alga Porphyra yezoensis (Bangiales, Rhodophyta) block thymine photodimer production. Mar Biotech 5:194–200
Monsalve-Bustamante YA, Puertas-Mejia MA, Mejia-Giraldo JC (2020) Comparison of the photoprotective effect between hydrolyzed and aglycones flavonoids as sunscreen: A systematic review. J Appl Pharm Sci 10:116–123
Montero L, Sánchez-Camargo AP, García-Cañas V, Tanniou A, Stiger-Pouvreau V, Russo M, Rastrelli L, Cifuentes A, Herrero M, Ibáñez E (2016) Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts. J Chromatogr A 1428:115–125
Nagayama K, Iwamura Y, Shibata T, Hirayama I, Nakamura T (2002) Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J Antimicrob Chemother 50:889–893
Naidoo K, Birch-Machin MA (2017) Oxidative stress and ageing: the influence of environmental pollution, sunlight and diet on skin. Cosmetics 4:4
Nasirideen S, Kaş H, Öner F, Alpar R, Hıncal A (1998) Naproxen incorporated lipid emulsions. I. Formulation and stability studies. J Clin Pharm Ther 23:57–65
Obluchinskaya E, Daurtseva A, Pozharitskaya O, Flisyuk E, Shikov A (2019) Natural deep eutectic solvents as alternatives for extracting phlorotannins from brown algae. Pharm Chem J 53:243–247
Pallela R, Na-Young Y, Kim S-K (2010) Anti-photoaging and photoprotective compounds derived from marine organisms. Mar Drugs 8:1189–1202
Pangestuti R, Siahaan EA, Kim S-K (2018) Photoprotective substances derived from marine algae. Mar Drugs 16:399
Pangestuti R, Shin K-H, Kim S-K (2021) Anti-photoaging and potential skin health benefits of seaweeds. Mar Drugs 19:172
Pereira L (2018) Seaweeds as source of bioactive substances and skin care therapy—Cosmeceuticals, algotheraphy, and thalassotherapy. Cosmetics 5:68
Poulose N, Sajayan A, Ravindran A, Sreechithra T, Vardhan V, Selvin J, Kiran GS (2020) Photoprotective effect of nanomelanin-seaweed concentrate in formulated cosmetic cream: With improved antioxidant and wound healing properties. J Photochem Photobiol B 205:111816
Pratama G, Yanuarti R, Ilhamdy A, Suhana M (2019) Formulation of sunscreen cream from Eucheuma cottonii and Kaempferia galanga (Zingiberaceae). IOP Conf Ser: Earth Environ Sci 278:012062
Pumival P, Tadtong S, Athikomkulchai S, Chittasupho C (2020) Antifungal activity and the chemical and physical stability of microemulsions containing Citrus hystrix DC leaf oil. Nat Prod Commun 15:1934578X20957755
Quintana Lazópulos S, Svarc F, Sagrera G, Dicelio L (2018) Absorption and photo-stability of substituted dibenzoylmethanes and chalcones as UVA filters. Cosmetics 5:33
Sabzevari N, Qiblawi S, Norton SA, Fivenson D (2021) Sunscreens: UV filters to protect us: Part 1: Changing regulations and choices for optimal sun protection. Int J Womens Dermatol 7:28–44
Sadeghi N, Behzad M, Jannat B, Hajimahmoodi M, Oveisi MR, Adibhashemi N, Moshkafshan M (2019) Investigation of total antioxidant capacity and total poly phenols of black tea in Iranian markets. J Food Safet Hyg 5:196–201
Saewan N, Jimtaisong A (2015) Natural products as photoprotection. J Cosm Dermatol 14:47–63
Salavkar SM, Tamanekar RA, Athawale RB (2011) Antioxidants in skin ageing–Future of dermatology. Int J Green Pharm 5(3). https://doi.org/10.4103/0973-8258.91221
Sambandan DR, Ratner D (2011) Sunscreens: an overview and update. J Am Acad Dermatol 64:748–758
Sayre RM, Agin PP, LeVee GJ, Marlowe E (1979) A comparison of in vivo and in vitro testing of sunscreening formulas. Photochem Photobiol 29:559–566
Schoenwaelder ME (2002) Physode distribution and the effect of thallus sunburn in Hormosira banksii (Fucales, Phaeophyceae). Bot Mar 45:262–266
Soleimani S, Pirian K, Zarei Jeliani Z, Arman M, Yousefzadi M (2018) Bioactivity assessment of selected seaweeds from the Persian Gulf. Iran J Aquat Ecol 7:25–38
Stiger-Pouvreau V, Guerard F (2018) Bio-inspired molecules extracted from marine macroalgae: a new generation of active ingredients for cosmetics and human health. In: Le Barre S, Bates SS (eds) Blue biotechnology: production and use of marine Molecules, vol 2. Wiley, NY, pp 709–746
Stiger-Pouvreau V, Jegou C, Cerantola S, Guérard F, Le Lann K (2014) Phlorotannins in Sargassaceae species from Brittany (France): interesting molecules for ecophysiological and valorisation purposes. Adv Bot Res 71:379–411
Surget G, Stiger-Pouvreau V, Le Lann K, Kervarec N, Couteau C, Coiffard LJ, Gaillard F, Cahier K, Guérard F, Poupart N (2015) Structural elucidation, in vitro antioxidant and photoprotective capacities of a purified polyphenolic-enriched fraction from a saltmarsh plant. J Photochem Photobiol B 143:52–60
Suwandi R, Anwar E, Maharany F, Hidayat T (2020) Characterization and formulation of sunscreen from seaweed Padina australis and Euchema cottonii slurry. IOP Conf Ser: Earth Environ Sci 404:012051
Svobodová A, Psotová J, Walterová D (2003) Natural phenolics in the prevention of UV-induced skin damage. A review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 147:137–145
Swanson AK, Druehl LD (2002) Induction, exudation and the UV protective role of kelp phlorotannins. Aquat Bot 73:241–253
Thiyagarasaiyar K, Mahendra CK, Goh B-H, Gew LT, Yow Y-Y (2021) UVB radiation protective effect of brown Alga Padina australis: a potential cosmeceutical application of Malaysian Seaweed. Cosmetics 8:58
Thomas P, Swaminathan A, Lucas RM (2012) Climate change and health with an emphasis on interactions with ultraviolet radiation: a review. GCB 18:2392–2405
Tierney MS, Smyth TJ, Rai DK, Soler-Vila A, Croft AK, Brunton N (2013) Enrichment of polyphenol contents and antioxidant activities of Irish brown macroalgae using food-friendly techniques based on polarity and molecular size. Food Chem 139:753–761
Vasconcelos JB, de Vasconcelos ER, Urrea-Victoria V, Bezerra PS, Reis TN, Cocentino AL, Navarro DM, Chow F, Areces AJ, Fujii MT (2019) Antioxidant activity of three seaweeds from tropical reefs of Brazil: potential sources for bioprospecting. J Appl Phycol 31:835–846
Vo TS, Kim SK, Ryu B, Ngo DH, Yoon NY, Bach LG, Hang NT, Ngo DN (2018) The suppressive activity of Fucofuroeckol-A derived from brown algal Ecklonia stolonifera Okamura on UVB-induced mast cell degranulation. Mar Drugs 16:1
Wang SQ, Stanfield JW, Osterwalder U (2008) In vitro assessments of UVA protection by popular sunscreens available in the United States. J Am Acad Dermatol 59:934–942
Wang L, Je J-G, Yang H-W, Jeon Y-J, Lee S (2021) Dieckol, an algae-derived phenolic compound, suppresses UVB-induced skin damage in human dermal fibroblasts and its underlying mechanisms. Antioxidants 10:352
Wang T, Jónsdóttir R, Liu H, Gu L, Kristinsson HG, Raghavan S, Ólafsdóttir G (2012) Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus. J Agri Food Chem 60:5874–5883
Wasitaatmadja SM (1997) Penuntun ilmu kosmetik medik. Jakarta: Penerbit Universitas Indonesia 3:58–59
Ye Y, Ji D, You L, Zhou L, Zhao Z, Brennan C (2018) Structural properties and protective effect of Sargassum fusiforme polysaccharides against ultraviolet B radiation in hairless Kun Ming mice. J Funct Foods 43:8–16
Zhang J-J, Li Y, Lin S-J, Li H-B (2018a) Green extraction of natural antioxidants from the Sterculia nobilis fruit waste and analysis of phenolic profile. Molecules 23:1059
Zhang R, Yuen AK, Magnusson M, Wright JT, de Nys R, Masters AF, Maschmeyer T (2018b) A comparative assessment of the activity and structure of phlorotannins from the brown seaweed Carpophyllum flexuosum. Algal Res 29:130–141
Zubia M, Fabre MS, Kerjean V, Le Lann K, Stiger-Pouvreau V, Fauchon M, Deslandes E (2009) Antioxidant and antitumoural activities of some Phaeophyta from Brittany coasts. Food Chem 116:693–701
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This research was financially supported by Iran National Science Foundation (INSF), Tehran, Iran (grant number: 96003639).
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Soleimani, S., Yousefzadi, M., Nezhad, S.B.M. et al. Evaluation of fractions extracted from Polycladia myrica: biological activities, UVR protective effect, and stability of cream formulation based on it. J Appl Phycol 34, 1763–1777 (2022). https://doi.org/10.1007/s10811-022-02705-2
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DOI: https://doi.org/10.1007/s10811-022-02705-2