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Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina japonica

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Abstract

Natural hybridization can play a significant role in evolutionary processes and influence the adaptive diversification and speciation of brown seaweeds. However, this phenomenon is as yet unknown in Saccharina kelps. Saccharina angustata and two varieties of Saccharina japonica (S. japonica var. japonica and S. japonica var. diabolica) partly overlap in distribution along the Pacific coast of Hokkaido, which makes them a good model system to study hybridization and introgression among species of the genus Saccharina. Based on 13 highly variable nuclear microsatellites and a mitochondrial marker, we assessed the genetic diversity levels of S. angustata for the first time and populations from Muroran to Shiranuka (western part of the Pacific coast in Hokkaido) exhibited highest genetic diversity. Genetic diversity of S. japonica was higher in S. japonica var. japonica as compared with S. japonica var. diabolica. There was significant genetic differentiation (FST > 0.25, p < 0.05) between S. japonica and S. angustata based on both markers. Moreover, there was poor genetic connectivity and limited interspecific hybridization among these closely related Saccharina species. Ecological niche models projected a northward expansion of both S. japonica and S. angustata under future climate scenarios and a range overlap between two species along the coast of Okhotsk Sea in Kamchatka Peninsula. The interspecific hybridization and genetic diversity among these kelps provide insights for kelp selection and cultivation as well as future conservation strategies of wild stocks.

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Acknowledgments

We thank Zhongmin Sun, Gang Fu, and Jing Wang for their assistance in kelp collections.

Funding

This study was supported by the National Natural Science Foundation of China (31900279), Shandong Provincial Natural Science Foundation (ZR2019BC024), Bilateral Joint Research Project between China and Japan (2017YFE0130900), and the Foundation for Science and Technology (FCT) of Portugal through the project UID/Multi/04326/2020 and the transitional norm (DL57/2016/ CP1361/CT0035).

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China

    Jie Zhang, Zi-Min Hu, Jianting Yao & Delin Duan

  2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

    Jie Zhang, Zi-Min Hu, Jianting Yao & Delin Duan

  3. Field Science Centre for Northern Biosphere, Hokkaido University, Sapporo, Japan

    Norishige Yotsukura

  4. Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway

    Alexander Jueterbock

  5. Centre of Marine Sciences, University of Algarve, Gambelas, Faro, Portugal

    Jorge Assis

  6. Muroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran, Japan

    Chikako Nagasato

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  1. Jie Zhang
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Zhang, J., Yotsukura, N., Jueterbock, A. et al. Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina japonica. J Appl Phycol 33, 693–702 (2021). https://doi.org/10.1007/s10811-020-02300-3

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