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Temperature and salinity effects on growth and fatty acid composition of a halophilic diatom, Amphora sp. MUR258 (Bacillariophyceae)

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Abstract

Diatoms are of great interest for large-scale cultivation due to their high lipid content. The ability to grow over a wide range of salinities is also of great advantage. We studied the effect of temperature and salinity on the growth, lipids and fatty acid profiles of a newly isolated halophilic diatom Amphora sp. MUR 258. Amphora sp. MUR 258 is unusual in that it grows over a wide range of temperatures (24–35 °C) and salinities (7–12% (w/v) NaCl). The highest specific growth rate (SGR; 0.607 ± 0.017 day−1) was achieved at 7% NaCl at 35 °C, and the lowest SGR (0.433 ± 0.087 day−1) was obtained at 9% NaCl at 25 °C. The cells contained more lipids in the exponential phase, except when grown at 12% NaCl where the lipid content was higher in the stationary phase. The alga achieved its highest lipid content (57.69 ± 2.039% ash-free dry weight (AFDW) when grown at 7% NaCl at 25 °C and the lowest (34.43 ± 3.955% AFDW) obtained at 12% NaCl at 35 °C. The highest biomass productivity (0.171 ± 0.017 gAFDW L−1 day−1) and the lipid productivity (0.062 ± 0.017 gAFDW L−1 day−1) were achieved when the Amphora were grown at 9% NaCl at 35 °C and 7% at 25 °C, respectively. Irrespective of the growth conditions, the predominant fatty acids of Amphora sp. MUR 258 were palmitic acid (C16:0), stearic acid (C18:0), palmitoleic acid (C16:1) and oleic acid (C18:1), as well as low quantities of eicosapentaenoic acid (C20:5).

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Funding

This project was financially supported by Murdoch University. Indrayani received a PhD Scholarship from AusAID (Australian Agency for International Development).

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Authors and Affiliations

  1. Algae R&D Centre, School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia

    Indrayani Indrayani, Navid R. Moheimani, Karne de Boer & Michael A. Borowitzka

  2. Faculty of Fisheries and Marine Science, University of Halu Oleo, Kendari, 93232, Indonesia

    Indrayani Indrayani

  3. School of Engineering and Information Technology, Murdoch University, Murdoch, WA, 6150, Australia

    Parisa A. Bahri

  4. Centre for Sustainable Aquatic Ecosystems, Harry Butler Institute, Murdoch University, Murdoch, WA, 6150, Australia

    Navid R. Moheimani

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  1. Indrayani Indrayani
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Correspondence to Michael A. Borowitzka.

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Indrayani, I., Moheimani, N.R., de Boer, K. et al. Temperature and salinity effects on growth and fatty acid composition of a halophilic diatom, Amphora sp. MUR258 (Bacillariophyceae). J Appl Phycol 32, 977–987 (2020). https://doi.org/10.1007/s10811-020-02053-z

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  • DOI: https://doi.org/10.1007/s10811-020-02053-z

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