Abstract
We investigated the extent to which nitrogenous and phosphorus nutrients from liquid anaerobic digestates could be recycled for photosynthetic growth of a microalga, Scenedesmus sp. AMDD. Digestates recovered from the anaerobic digestion of cow manure and swine manure and a co-digestion of swine manure and algal biomass were diluted in distilled water and used for algal growth with and without supplemental CO2 addition. Nutrient assimilation and final biomass yield were retarded in all but the swine manure/algae co-digestate cultures supplemented with high CO2. Swine manure digestate cultures supplemented with the typical complement of micronutrients normally added with a commonly used growth medium or with Fe/EDTA failed to grow any better than unamended controls. When the culture medium was prepared by blending swine manure digestate with 25 or 50 % algal biomass digestate, diluting it with lake water or by supplementing with magnesium, nutrient assimilation and final algal biomass yields were maximized, indicating that magnesium was critically limiting for algal growth in swine manure digestates. Magnesium amendment thus appears to be essential if nutrients from swine manure digestates are recycled for algal growth. No such requirement is necessary for recycling nutrients from digestates generated wholly or in part from algal biomass.
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References
Blier R, Laliberté G, de la Noüe J (1995) Tertiary treatment of cheese factory anaerobic effluent with Phormidium bohneri and Micractinium pusillum. Bioresour Technol 52:151–155
Bold HC (1949) The morphology of Chlamydomonas chlamydogama sp. nov. Bull Torrey Bot Club 76:101–108
Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306
Collet P, Hélias A, Lardon L, Ras M, Goy R-A, Steyer J-P (2011) Life cycle assessment of microalgae culture coupled to biogas production. Bioresour Technol 102:207–214
de la Noüe J, Bassères A (1989) Biotreatment of anaerobically digested swine manure with microalgae. Biol Wastes 29:17–31
Golueke CG, Oswald WJ (1959) Biological conversion of light energy to the chemical energy of methane. Appl Microbiol 7:219–227
Golueke CG, Oswald WJ, Gotaas HB (1957) Anaerobic digestion of algae. Appl Microbiol 5:47–55
Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of marine invertebrate animals. Plenum, New York, pp 26–60
McGinn PJ, Dickinson KE, Bhatti S, Frigon J-C, Guiot SR, O’Leary SJB (2011) Integration of microalgae cultivation with industrial waste remediation for biofuel and bioenergy production: opportunities and limitations. Photosynth Res 109:231–247
McGinn PJ, Dickinson KE, Park KC, Whitney CG, MacQuarrie SP, Black FJ, Frigon J, Guiot SR, O’Leary SJB (2012) Assessment of the bioenergy and bioremediation potentials of the microalga Scenedesmus sp. AMDD cultivated in municipal wastewater effluent in batch and continuous mode. Algal Res 2:155–165
Olguin EJ, Hernández B, Araus A, Camacho R, González R, Ramírez ME, Galicia S, Mercado G (1994) Simultaneous high-biomass protein production and nutrient removal using Spirulina maxima in sea water supplemented with anaerobic effluents. World J Microbiol Biotechnol 10:576–578
Park J, Jin H-F, Lim B-R, Park K-Y, Lee K (2010) Ammonia removal from anaerobic digestion effluent of livestock waste using green algal Scenedesmus sp. Bioresour Technol 101:8649–8657
Park KC, Whitney C, McNichol J, Dickinson KE, MacQuarrie S, Skrupski BP, Zhou J, Wilson K, O’Leary SJB, McGinn PJ (2011) Mixotrophic and photoautotrophic cultivation of 14 microalgae isolates from Saskatchewan, Canada: potential applications for wastewater remediation for biofuel production. J Appl Phycol 24:339–348
Ras M, Lardon L, Sialve B, Bernet N, Steyer J-P (2011) Experimental study on a coupled process of production an anaerobic digestion of Chlorella vulgaris. Bioresour Technol 102:200–206
Sialve B, Bernet N, Bernard O (2009) Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable. Biotechnol Adv 27:409–416
Stantec (2012) An analysis of the HRM lakes water quality monitoring program data (2006–2011). File no. 121510918. Stantec, Dartmouth
Vergara-Fernández A, Vargas G, Alarcón N, Velasco A (2008) Evaluation of marine algae as a source of biogas in a two-stage anaerobic reactor system. Biomass Bioenergy 32:338–344
Wang L, Yecong L, Chen P, Min M, Chen Y, Zhu J, Ruan R (2010) Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresour Technol 101:2623–2628
Acknowledgments
Thanks to the technical assistance of Mac MacAlpine and Lucas McNeal (University of Guelph, Ridgetown) and to Laura Garrison (NRC, Ketch Harbour). This is NRC publication no. 50506.
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Bjornsson, W.J., Nicol, R.W., Dickinson, K.E. et al. Anaerobic digestates are useful nutrient sources for microalgae cultivation: functional coupling of energy and biomass production. J Appl Phycol 25, 1523–1528 (2013). https://doi.org/10.1007/s10811-012-9968-0
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DOI: https://doi.org/10.1007/s10811-012-9968-0