Abstract
The amino acid profile of the red microalga Porphyridium cruentum and its protein extract have been determined in order to assess the nutritional quality of this biomass for human consumption. Total protein determined by elemental analysis represented 56 % of its dry weight. Hydro-soluble proteins extracted at pH 12 and 40 °C were analysed by the Lowry method giving 47 %, which represented 84 % of total protein per dry weight. The amino acid sequence of the biomass and the protein extract was composed of a set of essential (39 % for the former and 37 % for the latter) and non-essential amino acids (61 % for the former and 63 % for the latter) that compares favourably with the standard protein/amino acid requirements proposed by Food and Agricultural Organisation and World Health Organisation.
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References
Adda M, Merchuk JC, Arad S (1986) Effect of nitrate on growth and production of cell-wall polysaccharide by the unicellular red alga Porphyridium. Biomass 10:131–140
Arad S, Adda M, Cohen E (1985) The potential of production of sulfated polysaccharides from Porphyridium. Plant Soil 89:117–127
Arad S, Friedman O, Rotem A (1988) Effect of nitrogen on polysaccharide production in a Porphyridium sp. Appl Environ Microbiol 54:2411–2414
Barbarino E, Lourenço SO (2005) An evaluation of methodologies for extraction and quantification of protein of marine macro-and microalgae. J Appl Phycol 17:447–460
Becker EW (1994) Microalgae biotechnology and microbiology. Cambridge University Press, Cambridge, p 180
Berge JP, Debiton E, Dumay J, Durand P, Barthomeuf C (2002) In vitro anti-inflammatory and anti-proliferative activity of sulfolipids from the red alga Porphyridium cruentum. J Agric Food Chem 50:6227–6232
Bermejo Roman RB, Alvarez-Pez JM, Acien Fernandez FG, Molina Grima E (2002) Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum. J Biotechnol 93:73–85
Cohen Z, Shiran D, Khozin I, Heimer YM (1997) Fatty acid unsaturation in the red alga Porphyridium cruentum. Is the methylene-interrupted nature of polyunsaturated fatty acids an intrinsic property of the desaturases? BBA-Lipids Lipid Met 1344:59–64
Crossman DJ, Clements KD, Cooper GJS (2000) Determination of protein for studies of marine herbivory: a comparison of methods. J Exp Mar Biol Ecol 244:45–65
Diniz GS, Barbarino E, Oiano-Neto J, Pacheco S, Lourenço SO (2011) Gross chemical profile and calculation of nitrogen-to-protein conversion factors for five tropical seaweeds. Am J Plant Sci 2:287–296
Ducret A, Sidler W, Frank G, Zuber H (1994) The complete amino acid sequence of R-phycocyanin-I α and β subunits from the red alga Porphyridium cruentum. Eur J Biochem 221:563–580
Durmaz Y, Monteiro M, Bandarra N, Gkpinar O (2007) The effect of low temperature on fatty acid composition and tocopherols of the red microalga, Porphyridium cruentum. J Appl Phycol 19:223–227
FAO (2008) A review on culture, production and use of Spirulina as food for humans and feeds for domestic animals and fish. FAO Fish Aquacult, Rome, p 20
Fradique M, Batista AP, Nunes MC, Gouveia L, Bandarra NM, Raymundo A (2010) Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: preparation and evaluation. J Sci Food Agric 90:1656–1664
Fuentes MM, Fernández GG, Pérez JA, Guerrero JJ (2000) Biomass nutrient profiles of the microalga Porphyridium cruentum. Food Chem 70:345–353
Geresh S, Arad S (1991) The extracellular polysaccharides of the red microalgae: chemistry and rheology. Bioresour Technol 38:195–201
Geresh S, Mamontov A, Weinstein J (2002) Sulfation of extracellular polysaccharides of red microalgae: preparation, characterization and properties. J Biochem Biophys Methods 50:179–187
Ginzberg A, Cohen M, Sod-Moriah UA, Shany S, Rosenstrauch A, Arad SM (2000) Chickens fed with biomass of the red microalga Porphyridium sp. have reduced blood cholesterol level and modified fatty acid composition in egg yolk. J Appl Phycol 12:325–330
González López CV, García MDCC, Fernández FGA, Bustos CS, Chisti Y, Sevilla JMF (2010) Protein measurements of microalgal and cyanobacterial biomass. Bioresour Technol 101:7587–7591
Heaney-Kieras J, Chapman D (1976) Structural studies on the extracellular polysaccharide of the red alga, Porphyridium cruentum. Carbohyd Res 52:169–177
Hemerick G (1973) Culture methods and growth measurements. In: Stein JR (ed) Handbook of phycological methods. Cambridge University Press, Cambridge, pp 259–260
Khozin-Goldberg I, Yu H, Adlerstein D, Didi-Cohen S, Heimer Y, Cohen Z (2000) Triacylglycerols of the red microalga Porphyridium cruentum can contribute to the biosynthesis of eukaryotic galactolipids. Lipids 35:881–889
Ley AC, Butler WL, Bryant DA, Glazer AN (1977) Isolation and function of allophycocyanin B of Porphyridium cruentum. Plant Physiol 59:974–980
Loubiere K, Pruvost J, Aloui F, Legrand J (2011) Investigations in an external-loop airlift photobioreactor with annular light chambers and swirling flow. Chem Eng Res Des 89:164–171
Lourenço SO, Barbarino E, Marquez UML, Aidar E (1998) Distribution of intracellular nitrogen in marine microalgae: basis for the calculation of specific nitrogen-to-protein conversion factors. J Phycol 34:798–811
Lourenço SO, Barbarino E, Lavan PL, Lanfer Marquez UM, Aidar E (2004) Distribution of intracellular nitrogen in marine microalgae: calculation of new nitrogen-to-protein conversion factors. Eur J Phycol 39:17–32
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mahan LK, Escott-Stump S (2004) Krause's Food, nutrition and diet therapy. Saunders Elsevier, Pennsylvania, p 66
Mariotti F, Tomé D, Mirand PP (2008) Converting nitrogen into protein: beyond 6.25 and Jones' factors. Crit Rev Food Sci 48:177–184
Moore S, Stein WH (1948) Photometric ninhydrin method for use in the chromatography of amino acids. J Biol Chem 176:367–388
Patil V, Källqvist T, Olsen E, Vogt G, Gislerød H (2007) Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquacult Int 15:1–9
Shiran D, Khozin I, Heimer Y, Cohen Z (1996) Biosynthesis of eicosapentaenoic acid in the microalga Porphyridium cruentum: the use of externally supplied fatty acids. Lipids 31:1277–1282
WHO (2007) Protein and amino acid requirements in human nutrition. World Health Organisation, Geneva, p 284
Acknowledgments
The authors would like to thank Alpha Biotech for providing the biomass. This work was supported by the French National Research Agency (ANR) in the context of Algoraffinerie project.
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Safi, C., Charton, M., Pignolet, O. et al. Evaluation of the protein quality of Porphyridium cruentum . J Appl Phycol 25, 497–501 (2013). https://doi.org/10.1007/s10811-012-9883-4
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DOI: https://doi.org/10.1007/s10811-012-9883-4