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Carotenoid-to-chlorophyll ratio as a proxy for assay of total fatty acids and arachidonic acid content in the green microalga Parietochloris incisa

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Abstract

The relationships between pigment (carotenoid and chlorophyll) content with accumulation of total fatty acids (TFA) and arachidonic acid (AA) were studied in the green microalga Parietochloris incisa (Trebouxiophyceae, Chlorophyta) grown under different PFDs (35, 200, and 400 μmol photons m−2 s−1) and nitrogen availabilities. The growth of P. incisa under higher light and nitrogen deficiency was accompanied by accumulation of FA, an increase in carotenoid and a decline in chlorophyll content. It was found that the carotenoid-to-chlorophyll ratio (but not the individual pigment content) correlates closely with the volumetric content of both TFA and AA. Analysis of scattering-compensated absorption spectra of P. incisa suspensions revealed their tight relationship in the blue-green range of the spectrum with the carotenoid-to-chlorophyll ratio, TFA, and AA content. These findings allowed the development of algorithms for the non-destructive assay of TFA and AA in cell suspensions in the ranges of 0.09–3.04 and 0.04–1.7 μg mL−1, with accuracy of 0.06 and 0.01 μg mL−1, respectively, via analytically measured carotenoid-to-chlorophyll ratio and using the ratio of absorption coefficients at 510 and 678 nm, with accuracy of 0.07 and 0.02 μg mL−1, respectively. The feasibility of obtaining essential spectral information concerning the physiological condition of P. incisa using a standard spectrophotometer is also shown.

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Abbreviations

Ã(λ):

scattering-free absorption spectrum

AA:

arachidonic acid

TAG:

triacylglycerols

TFA:

total fatty acids

PUFA:

polyunsaturated fatty acids

References

  • Ben-Amotz A, Katz A, Avron M (1982) Accumulation of β-carotene in halotolerant algae: purification and characterization of β-carotene rich globules from Dunaliella bardawil (Chlorophyceae). J Phycol 18:529–37. doi:10.1111/j.1529-8817.1982.tb03219.x

    Article  CAS  Google Scholar 

  • Borowitzka MA (2005) Carotenoid production using microorganisms. In: Cohen Z, Ratledge C (eds) Single cell oils. AOCS Press, Champaign, IL, pp 124–137

    Google Scholar 

  • Borowitzka MA, Borowitzka LJ (eds) (1988) Micro-algal biotechnology. Cambridge University Press, Cambridge

  • Borowitzka MA, Siva CJ (2007) The taxonomy of the genus Dunaliella (Chlorophyta, Dunaliellales) with emphasis on the marine and halophilic species. J Appl Phycol 19:567–590. doi:10.1007/s10811-007-9171-x

    Article  Google Scholar 

  • Boussiba S (2000) Carotenogenesis in the green alga Haematococcus pluvialis: cellular physiology and stress response. Physiol Plant 108:111–117. doi:10.1034/j.1399-3054.2000.108002111.x

    Article  CAS  Google Scholar 

  • Cheng-Wu Z, Cohen Z, Khozin-Goldberg I, Richmond A (2002) Characterization of growth and arachidonic acid production of Parietochloris incisa comb. nov (Trebouxiophyceae, Chlorophyta). J Appl Phycol 14:453–460. doi:10.1023/A:1022375110556

    Article  Google Scholar 

  • Crawford MA, Golfetto I, Ghebremeskel K, Min Y, Moodley T, Poston L et al (2003) The potential role for arachidonic and docosahexaenoic acids in protection against some central nervous system injuries in preterm infants. Lipids 38:303–315. doi:10.1007/s11745-003-1065-1

    Article  PubMed  CAS  Google Scholar 

  • Gitelson A, Qiuang H, Richmond A (1996) Photic volume in photoreactors supporting ultrahigh population densities of the photoautotroph Spirulina platensis. Appl Environ Microbiol 62:1570–1573

    PubMed  CAS  Google Scholar 

  • Gitelson AA, Grits YA, Etzion D, Ning Z, Richmond A (2000) Optical properties of Nannochloropsis sp and their application to remote estimation of cell mass. Biotechnol Bioeng 69:516–525. doi:10.1002/1097-0290(20000905)69:5<516::AID-BIT6>3.0.CO;2-I

    Article  PubMed  CAS  Google Scholar 

  • Hansen J, Schade D, Harris C, Merkel K, Adamkin D, Hall R et al (1997) Docosahexaenoic acid plus arachidonic acid enhance preterm infant growth. Prostaglandins Leukot Essent Fatty Acids 57:196

    Google Scholar 

  • Khozin-Goldberg I, Bigogno C, Shreshta P, Cohen Z (2002) Nitrogen starvation induces the accumulation of arachidonic acid in the freshwater green alga Parietochloris incisa (Trebuxiophyceae). J Phycol 38:991–994. doi:10.1046/j.1529-8817.2002.01160.x

    Article  CAS  Google Scholar 

  • Mendoza H, Martel A, Jimenez del Rio M, Garcia Reina G (1999) Oleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina. J Appl Phycol 11:15–19. doi:10.1023/A:1008014332067

    Article  CAS  Google Scholar 

  • Merzlyak MN, Naqvi KR (2000) On recording the true absorption and scattering spectrum of a turbid sample: application to cell suspensions of the cyanobacterium Anabaena variabilis. J Photochem Photobiol B 58:123–129. doi:10.1016/S1011-1344(00)00114-7

    Article  PubMed  CAS  Google Scholar 

  • Merzlyak MN, Chivkunova OB, Gorelova OA, Reshetnikova IV, Solovchenko AE, Khozin-Goldberg I et al (2007) Effect of nitrogen starvation on optical properties, pigments and arachidonic acid content of the unicellular green alga Parietochloris incisa (Trebouxiophyceae, Chlorophyta). J Phycol 43:833–843. doi:10.1111/j.1529-8817.2007.00375.x

    Article  CAS  Google Scholar 

  • Merzlyak MN, Chivkunova OB, Maslova IP, Naqvi RK, Solovchenko AE, Klyachko-Gurvich GL (2008) Light absorption and scattering by cell suspensions of some cyanobacteria and microalgae. Russ J Plant Physiol 54:420–442. doi:10.1134/S1021443708030199

    Article  Google Scholar 

  • Pick U (1998) Dunaliella—a model extremophilic alga. Isr J Plant Sci 46:131–139

    Google Scholar 

  • Rabbani S, Beyer P, Lintig J, Hugueney P, Kleinig H (1998) Induced β-carotene synthesis driven by triacylglycerol deposition in the unicellular alga Dunaliella bardawil. Plant Physiol 116:1239–1248. doi:10.1104/pp.116.4.1239

    Article  PubMed  CAS  Google Scholar 

  • Shibata K (1973) Dual wavelength scanning of leaves and tissues with opal glass. Biochim Biophys Acta 304:249–259

    PubMed  CAS  Google Scholar 

  • Solovchenko AE, Khozin-Goldberg I, Didi-Cohen S, Cohen Z, Merzlyak MN (2008a) Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa. J Appl Phycol 20:245–225. doi:10.1007/s10811-007-9233-0

    Article  CAS  Google Scholar 

  • Solovchenko AE, Khozin-Goldberg I, Didi-Cohen S, Cohen Z, Merzlyak MN (2008b) Effects of light and nitrogen starvation on the content and composition of carotenoids of the green microalga Parietochloris incisa. Russ J Plant Physiol 53:455–462. doi:10.1134/S1021443708040043

    Article  Google Scholar 

  • Vonshak A (1985) Microalgae: laboratory growth techniques and outdoor biomass production. In: Coombs J, Hall DO, Long SP, Scurlock JMO (eds) Techniques in bioproductivity and photosynthesis. Pergamon Press, Oxford, pp 188–203

  • Wang B, Zarka A, Trebst A, Boussiba S (2003) Astaxanthin accumulation in Haematococcus pluvialis (Chlorophyceae) as an active photoprotective process under high irradiance. J Phycol 39:1116–1124. doi:10.1111/j.0022-3646.2003.03-043.x

    Article  CAS  Google Scholar 

  • Zhekisheva M, Boussiba S, Khozin-Goldberg I, Zarka A, Cohen Z (2002) Accumulation of oleic acid in Haematococcus pluvialis (Chlorophyceae) under nitrogen starvation or high light is correlated with that of astaxanthin esters. J Phycol 38:325–331. doi:10.1046/j.1529-8817.2002.01107.x

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported in part by fellowships from the Blaustein Center for Scientific Cooperation (BCSC) to A.E.S. and M.N.M. The financial support from the Russian Foundation for Basic Research (Grant # 06-04-48883) and the Russian President’s Grant Council (Ministry of Science of the Russian Federation, Grant # MK-3433.2008.4) is also acknowledged.

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Authors and Affiliations

  1. Department of Physiology of Microorganisms, Faculty of Biology, Moscow State University, 119991, GSP-1 Moscow, Russia

    A. E. Solovchenko & M. N. Merzlyak

  2. Microalgal Biotechnology Laboratory, The Albert Katz Department of Drylands Biotechnologies, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede-Boker Campus, 84990, Beersheba, Israel

    I. Khozin-Goldberg & Z. Cohen

Authors
  1. A. E. Solovchenko
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  2. I. Khozin-Goldberg
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  3. Z. Cohen
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  4. M. N. Merzlyak
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Correspondence to A. E. Solovchenko.

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Solovchenko, A.E., Khozin-Goldberg, I., Cohen, Z. et al. Carotenoid-to-chlorophyll ratio as a proxy for assay of total fatty acids and arachidonic acid content in the green microalga Parietochloris incisa . J Appl Phycol 21, 361–366 (2009). https://doi.org/10.1007/s10811-008-9377-6

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  • DOI: https://doi.org/10.1007/s10811-008-9377-6

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