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A novel electrochemical DNA-sensing nanoplatform based on supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels

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Abstract

In this paper, the application of supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels (SIL-g-(N)GAs), a novel electrode system, for the preparation of electrochemical DNA sensing platform is proposed. The super dispersion of SIL-g-(N)GAs in water makes it an ideal candidate for biological purposes such as gene delivery. In fact, SIL-g-(N)GAs/glassy carbon working electrode (GCE) can realize the simultaneous detection of all four DNA bases in double-stranded DNA without a prehydrolysis step. On the SIL-g-(N)GAs/GCE, due to the presence of SIL on the surface of three-dimensional nitrogen-doped graphene, the anchoring of the DNA probe can be achieved by electrostatic association of SIL cations with the DNA backbone readily. So, herein we report a novel strategy for DNA hybridization without any electroactive tags or intercalators and suggest the potential applications of SIL-g-(N)GAs/GCE in the label-free electrochemical detection of DNA hybridization or DNA damage for further research. Moreover, the influence of potentially interfering substances on the determination of DNA is investigated.

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Authors and Affiliations

  1. Faculty of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), 424 Hafez Ave, Tehran, 15875-4413, Iran

    Hanif Kazerooni & Bahram Nasernejad

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  1. Hanif Kazerooni
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  2. Bahram Nasernejad
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Correspondence to Bahram Nasernejad.

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Kazerooni, H., Nasernejad, B. A novel electrochemical DNA-sensing nanoplatform based on supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels. J Appl Electrochem 45, 1289–1298 (2015). https://doi.org/10.1007/s10800-015-0891-3

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  • DOI: https://doi.org/10.1007/s10800-015-0891-3

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