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The influencer effect of Dexmedetomidine on radioiodine relevant to lacrimal gland impairment

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Abstract

Purpose

To assess the potential influencing effects of Dexmedetomidine on impaired lacrimal glands after high-dose radioiodine treatment (RAI).

Methods

Thirty-six rats were arbitrarily separated into 3 groups: Sham, RAI, and Dexmedetomidine. Dexmedetomidine group was given Dexmedetomidine and RAI, the Sham group was given the same millimeters of saline, and the RAI group was given RAI only. All forms of lacrimal glands, including harderian glands (HG), extraorbital (EG), and intraorbital (IG) lacrimal glands, were evaluated for immunohistochemical, histopathologic assessments and also for tissue cytokines, oxidant and antioxidant levels.

Results

Dexmedetomidine significantly ameliorated histopathologic changes such as periacinar fibrosis, acinar atrophy, lymphocytic infiltration, ductal proliferation, lipofuscin-like accumulation, and nucleus changes caused by RAI in all lacrimal gland forms (p < 0.05 for all of the parameters). However, periductal fibrosis was improved significantly only in EG (p = 0.049), and mast cell infiltration was improved significantly only in IG (p = 0.038) in Dexmedetomidine groups. There was a significant decrease in the elevated caspase-3 and TUNEL levels after RAI administration in the Dexmedetomidine group in all lacrimal gland forms (p < 0.05 for all parameters). Dexmedetomidine attenuated NF-kb, TNF-α, and IL-6 levels significantly diminished total oxidant status and raised total antioxidant status levels (p < 0.05 for all parameters).

Conclusions

The results of this study demonstrated that following RAI, Dexmedetomidine diminished inflammation, tissue cytokine levels, and apoptosis and ameliorated impaired histopathologic patterns of the lacrimal glands.

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Funding

Declared none.

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Authors and Affiliations

  1. Department of Ophthalmology, University of Health Sciences, Ankara Training and Research Hospital, Ankara, Turkey

    Evin Şingar & Ayse Burcu

  2. Department of Nuclear Medicine, University of Health Sciences, Ankara Training and Research Hospital, Ankara, Turkey

    Aylin Akbulut, Gökhan Koca & Meliha Korkmaz

  3. Department of Physiopathology, Ankara University, Ankara, Turkey

    Nuray Yazihan

  4. Department of Nuclear Medicine, Hatay Mustafa Kemal University, Hatay, Turkey

    Hasan İkbal Atilgan

  5. Veterinary Faculty, Department of Pathology, Harran University, Sanliurfa, Turkey

    Nihat Yumuşak

  6. Faculty of Nursing, Ankara University, Ankara, Turkey

    Ayten Demir

Authors
  1. Evin Şingar
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  2. Aylin Akbulut
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  3. Gökhan Koca
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  4. Nuray Yazihan
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  5. Hasan İkbal Atilgan
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  6. Nihat Yumuşak
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  7. Ayten Demir
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  8. Ayse Burcu
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  9. Meliha Korkmaz
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Contributions

Singar, Akbulut wrote the main manuscript; Yazıhan, Yumusak and Demir prepared figures; Singar, Koca, Korkmaz, Burcu and Atılgan prepared the study, all authors reviewed the manuscript.

Corresponding author

Correspondence to Evin Şingar.

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Compliance with ethical standards

All procedures on animals were carried out by the Animal Ethics Committee. The Laboratory Animal Care Committee of the University of Health Sciences Ankara Training and Research Hospital reviewed and approved the experimental protocol (Report no:19.03.2020/609).

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Şingar, E., Akbulut, A., Koca, G. et al. The influencer effect of Dexmedetomidine on radioiodine relevant to lacrimal gland impairment. Int Ophthalmol 44, 115 (2024). https://doi.org/10.1007/s10792-024-03052-6

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