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Antibacterial effects of octadecyl trimethylammonium micelle–clay complex against bacterial eye pathogens: potential as a contact lens disinfectant

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Abstract

Aim

In this study, we utilized a micelle–clay complex composed of the surfactant octadecyltrimethylammonium bromide and montmorillonite clay and evaluated its antibacterial effects.

Methods

Using Pseudomonas aeruginosa, Staphylococcus epidermidis, and Micrococcus luteus, bactericidal assays were performed to determine the effects of ODTMA-clay complex on the viability of bacterial pathogen at various doses and different intervals of time. Cytotoxicity assays were performed to investigate ODTMA-clay complex effects on human cells, as determined by release of intracellular lactate dehydrogenase.

Results

The results revealed that ODTMA-clay complex abolished bacterial viability at 100 μg/mL within 45 min against P. aeruginosa, S. epidermidis, and M. luteus. Cytotoxicity assays revealed that ODTMA-clay complex exhibited minimal toxicity of the human cells.

Conclusion

Rapid and potent antibacterial effects of ODTMA micelle–clay complex were observed in vitro; however, research is needed to determine precise formulation of contact lens disinfectants comprising ODTMA micelle–clay complex. Additionally, studies should be conducted using in vivo models of keratitis, progressing to pre-clinical and clinical trials. ODTMA micelle–clay complex is an ideal candidate to be incorporated in a novel contact lens disinfectant given the cost-effectiveness and ease of application and can be incorporated as an effective preventative strategy.

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Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: American University of Sharjah and University of Sharjah.

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Authors and Affiliations

  1. College of Arts and Sciences, American University of Sharjah, University City, Sharjah, United Arab Emirates

    Ruqaiyyah Siddiqui, Rida Arshad Shah, Noor Akbar, Mustafa Khamis & Mohamed Yehia Abouleish

  2. Department of Chemical Engineering, College of Engineering, American University of Sharjah, University City, Sharjah, United Arab Emirates

    Taleb Ibrahim

  3. Department of Clinical Sciences, College of Medicine, University of Sharjah, PO Box 27272, University City, Sharjah, United Arab Emirates

    Naveed Ahmed Khan

Authors
  1. Ruqaiyyah Siddiqui
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  2. Rida Arshad Shah
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  3. Noor Akbar
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  4. Mustafa Khamis
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  5. Taleb Ibrahim
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  6. Mohamed Yehia Abouleish
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  7. Naveed Ahmed Khan
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Contributions

RS and NAK conceived the idea amid discussions with MK, TI and MA. RAS and NA carried out all experiments under the supervision of RS and NAK. NA and RS prepared the first draft of the manuscript. NAK corrected the manuscript. All authors approved the final manuscript.

Corresponding author

Correspondence to Naveed Ahmed Khan.

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Conflict of interest

Author RS declares that she has no conflict of interest. Author MK declares that he has no conflict of interest. Author TI declares that he has no conflict of interest. Author NA declares that he has no conflict of interest. Author RAS declares that she has no conflict of interest. Author MA declares that he has no conflict of interest. Author NAK declares that he has no conflict of interest.

Ethical approval

No humans or tissues were used in this research. No animals were involved in this research.

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Cite this article

Siddiqui, R., Shah, R.A., Akbar, N. et al. Antibacterial effects of octadecyl trimethylammonium micelle–clay complex against bacterial eye pathogens: potential as a contact lens disinfectant. Int Ophthalmol 42, 939–944 (2022). https://doi.org/10.1007/s10792-021-02075-7

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  • DOI: https://doi.org/10.1007/s10792-021-02075-7

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