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Effect of antibiotic prophylaxis on Coagulase-negative Staphylococcus virulence factor profiles in patients undergoing cataract surgery

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Abstract

Purpose

In this prospective study, multiplex polymerase chain reaction (PCR) was used to identify genes encoding virulence factors (ica, atlE and mecA) in Coagulase-negative Staphylococcus (CNS) isolates from the ocular microbiota of patients undergoing cataract surgery and to investigate possible changes in the CNS profile due to antibiotic prophylaxis.

Methods

Between 09/2011 and 08/2013, patients undergoing cataract surgery were recruited at the Department of Ophthalmology, National University of Asuncion, Paraguay. In the eye to be operated on, patients received moxifloxacin 0.5 % eye drops four times at the day before surgery and a last drop 1 hour before surgery (T1). The other eye remained as control (T0). Conjunctival swabs were taken from both eyes 1 hour after the last drop. The presence of genes encoding biofilm formation (ica and atlE) and methicillin resistance (mecA) was detected by a multiplex PCR.

Results

Of the 162 patients (162 study eyes, 162 fellow eye as control group), 87 (53.7 %) eyes were positive for CNS at T0 yielding 96 CNS isolates; 70 eyes (43.2 %) were positive at T1 yielding 77 CNS isolates. For this study, 43 CNS isolates (44.8 %) from T0 and 45 (64.3 %) from T1 were used. Of the total isolates, 81.8 % (72/88) had at least one virulence factor gene (37/43 from T0 and 35/45 from T1) (p = 0.314). Simultaneous detection of ica and atlE genes was higher in T0 (58.0 %) than T1 (46.7 %), but the difference was not significant (p = 0.28).

Conclusion

A high frequency of genes encoding virulence factors was observed in the coagulase-negative Staphylococcus isolates. The use of moxifloxacin did not significantly modify the CNS virulence factor profiles.

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Acknowledgments

This work was supported in part by Georg and Hannelore Zimmerman, Foundation Munich, Germany.

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Authors and Affiliations

  1. Instituto de Investigaciones en Ciencias de la Salud (IICS), National University of Asunción, San Lorenzo, Paraguay

    Yolanda López, Margarita Samudio, Norma Fariña, Sonia Abente, Nilsa González-Britez & Florentina Laspina

  2. Department of Ophthalmology, National University of Asunción, San Lorenzo, Paraguay

    Verónica Castillo, Agustín Carron & Diógenes Cibils

  3. Department of Ophthalmology, Julius-Maximilians-University, Würzburg, Germany

    Martin M. Nentwich

  4. Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany

    Herminia Miño de Kaspar

Authors
  1. Yolanda López
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Correspondence to Margarita Samudio.

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Supported in part by “Georg and Hannelore Zimmermann Foundation,” Germany.

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López, Y., Samudio, M., Fariña, N. et al. Effect of antibiotic prophylaxis on Coagulase-negative Staphylococcus virulence factor profiles in patients undergoing cataract surgery. Int Ophthalmol 37, 929–937 (2017). https://doi.org/10.1007/s10792-016-0355-8

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