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Inhibitory Effects of Euscaphic Acid in the Atopic Dermatitis Model by Reducing Skin Inflammation and Intense Pruritus

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Abstract

Atopic dermatitis (AD) is a complex and multifactorial skin disease characterized by skin inflammation and intense pruritus. There are many commercially available treatments such as topical corticosteroids and immunosuppressants to treat of AD, but their effectiveness is limited, and frequent use of these treatments can cause serious side effects. Therefore, the development of new therapeutic agents is necessary for the treatment of AD. Hence, an alternative agent that was derived from natural products that are effective and safe for AD treatment was investigated using experimental models. The biological activity of euscaphic acid has anti-inflammatory, anticoagulant, and antioxidant effects. Despite the various biomedical properties of euscaphic acid, its therapeutic effects on AD have not been well studied. In this study, we investigated the effects of euscaphic acid on skin inflammation and pruritus in AD mouse model. The effects of euscaphic acid were investigated in activated human epidermal keratinocytes and leukemia T lymphoblast cell lines, and Dermatophagoides farina extract and 2,4-dinitrochlorobenzene-induced AD mouse model. Euscaphic acid ameliorated AD properties, such as the expression of inflammatory cytokines and activation of transcription factors. In addition, euscaphic acid reduced critical factors for pruritus such as immunoglobulin E hyperproduction, mast cell invasion, and interleukin-33 expression. Taken together, euscaphic acid could be a potent therapeutic agent for the treatment of AD.

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Avaiiability of Data and Material

The analyzed data sets presented in this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (2019M3A9H1103690, 2020M3A9D3038894, 2020R1A2C1010962, and 2017M3A9G8083382).

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Authors and Affiliations

  1. Cell & Matrix Research Institute, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

    Na-Hee Jeong, Young-Ae Choi & Sang-Hyun Kim

  2. Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea

    Soyoung Lee

  3. GHAM BioPharm Co. Ltd., College of Pharmacy, Kyungpook National University, Daegu, Republic of Korea

    Kyung-Sik Song

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  1. Na-Hee Jeong
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Contributions

Na-Hee Jeong: Conception and design of the research and wrote the manuscript. Soyoung Lee: Conception and design of the research. Young-Ae Choi: Analysis of data. Kyung-Sik Song: Analysis of data and wrote the manuscript. Sang-Hyun Kim: Conception and design of the research, analysis of data, and wrote the manuscript.

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Correspondence to Young-Ae Choi, Kyung-Sik Song or Sang-Hyun Kim.

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All animal experiments were approved by the Institutional Animal Care and Use Committee at Kyungpook National University and performed in strict accordance with the guidelines for the Public Health Service Policy on the Humane Care and Use of Laboratory Animals.

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Jeong, NH., Lee, S., Choi, YA. et al. Inhibitory Effects of Euscaphic Acid in the Atopic Dermatitis Model by Reducing Skin Inflammation and Intense Pruritus. Inflammation 45, 1680–1691 (2022). https://doi.org/10.1007/s10753-022-01652-x

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