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Association of miR-155, miR-187 and Inflammatory Cytokines IL-6, IL-10 and TNF-α in Chronic Opium Abusers

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Abstract

Substance use disorders are known to be associated with inflammation. However, the dynamics of inflammatory cytokines and microRNA in chronic opium use is yet unexplored. The current study determined the levels of inflammatory cytokines TNF-α, IL-6, IL-10 and immune-regulatory miR-155 and miR-187 expressions in chronic opioid use disorder. Adults (n = 48) meeting the 5th Edition of the DSM criteria regarding opioid use disorder and healthy controls (n = 46) were included in the study. Inflammatory cytokines IL-10, IL-6, and TNF-α were analyzed from serum samples, and peripheral blood mononuclear cells processed for miRNA expression. Cases showed significantly raised IL-10 and TNF-α and reduced IL-6. Dose-dependent upregulation of miR-155-5p and miR-187-5p was evident at opium dose >1500 g/month, with a corresponding increase of TNF-α and IL-10. MiR-155 showed a significant positive correlation with IL-6 and TNF-α levels, while miR-187 showed a significant negative association with TNF-α at ≥1000 g/month consumption. Therefore, increasing consumption of opium probably enhances inflammation leading to immunomodulation and aberrant expression of hsa-miR-155-5p and hsa-miR-187-5p in opioid use disorder.

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Funding

This study was funded by an internal grant from the Institutional Review and Ethics Board, AIIMS Jodhpur [AIIMS/Res(02)/2015-16/308].

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Authors and Affiliations

  1. Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Jodhpur, Rajasthan, India

    Purvi Purohit, Dipayan Roy & Praveen Sharma

  2. Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Gorakhpur, Uttar Pradesh, India

    Shailendra Dwivedi

  3. Department of Psychiatry, All India Institute of Medical Sciences (AIIMS), Jodhpur, Rajasthan, India

    Naresh Nebhinani

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Contributions

Purvi Purohit: Grant awarded, Study concept, study design, sample analysis, data collection and analysis, interpretation, drafting manuscript, manuscript editing. Dipayan Roy: Data collection and analysis, drafting manuscript, manuscript editing. Shailendra Dwivedi: study design, sample analysis, manuscript editing. Naresh Nebhinani: Patient recruitment, manuscript editing. Praveen Sharma: Data analysis, Manuscript editing.

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Correspondence to Purvi Purohit.

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Purohit, P., Roy, D., Dwivedi, S. et al. Association of miR-155, miR-187 and Inflammatory Cytokines IL-6, IL-10 and TNF-α in Chronic Opium Abusers. Inflammation 45, 554–566 (2022). https://doi.org/10.1007/s10753-021-01566-0

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