Abstract
Cardiac dysfunction is a major cause leading to multiple organ failure in sepsis. Beclin-1-dependent autophagy has been evidenced to exert protective effects on hearts in sepsis. However, the mechanisms on how Beclin-1 and autophagy are regulated remains enigmatic. To explore the detailed mechanisms controlling Beclin-1-dependent autophagy in septic heart and whether melatonin could protect against sepsis via regulating cardiac autophagy, adult Sprague-Dawley (SD) rats were subjected to cecal ligation and puncture (CLP) to induce sepsis. Rats were intraperitoneally administrated with 30 mg/kg melatonin within 5-min post-CLP surgery. Our data showed that sepsis induced Becline-1 acetylation and inhibited autophagy in hearts, resulting in impaired cardiac function. However, melatonin treatment facilitated Beclin-1 deacetylation and increased autophagy in septic hearts, thus improved cardiac function. Moreover, melatonin increased the expression and activity of Sirtuin 1 (Sirt1), and inhibition of Sirt1 abolished the protective effects of melatonin on Beclin-1 deacetylation and cardiac function. In conclusion, increased Beclin-1 acetylation was involved in impaired autophagy in septic hearts, while melatonin contributed to Beclin-1 deacetylation via Sirt1, leading to improved autophagy and cardiac function in sepsis. Our study sheds light on the important role of Beclin-1 acetylation in regulating autophagy in sepsis and suggests that melatonin is a potential candidate drug for the treatment of sepsis.
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This research was funded by the National Natural Science foundation of China (NSFC81700320), Chongqing Science and Technical Commission Basic Research Funding (CSTC 2019JCY-MSXMX0827) and Basic Research Training Project of the First Affiliated Hospital of Chongqing Medical University (PyJJ2017-11).
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Conceptualization, Xiao-Wen Wang; methodology, Qiang-Zhong Pi, Xiao-Wen Wang, Zhao-Lei Jian, Dan Chen; formal analysis, Xiao-Wen Wang, Qing-Chen Wu; investigation, Qiang-Zhong Pi, Xiao-Wen Wang, Zhao-Lei Jian, Dan Chen; writing—original draft preparation, Qiang-Zhong Pi; writing—review and editing, Xiao-Wen Wang; supervision, Qing-Chen Wu; funding acquisition, Xiao-Wen Wang. All authors have read and agreed to the published version of the manuscript.
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Pi, QZ., Wang, XW., Jian, ZL. et al. Melatonin Alleviates Cardiac Dysfunction Via Increasing Sirt1-Mediated Beclin-1 Deacetylation and Autophagy During Sepsis. Inflammation 44, 1184–1193 (2021). https://doi.org/10.1007/s10753-021-01413-2
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DOI: https://doi.org/10.1007/s10753-021-01413-2