Abstract
Sinomenine (SIN) has been shown to protect against IL-1β-induced chondrocyte apoptosis in vitro. However, the role of SIN in the anterior cruciate ligament transection (ACLT)-induced osteoarthritis (OA) mouse model and its underlying molecular mechanisms remain unclear. In the present study, the protective effect of SIN on ACLT-induced articular cartilage degeneration and IL-1β-induced chondrocyte apoptosis miR-223-3p/NLRP3 signaling regulation was investigated. Safranin O staining was performed to evaluate the pathological changes of articular cartilage. Chondrocyte apoptosis was measured with Annexin V-fluorescein isothiocyanate/polyimide (annexin V-FITC/PI) staining using flow cytometry. Gene and protein expression were detected by RT-qPCR and Western blotting, respectively. SIN administration markedly improved articular cartilage degradation in mice undergoing ACLT surgery. In addition, SIN treatment downregulated the levels of inflammatory cytokines and the protein expression of NLRP3 inflammasome components and upregulated the expression of miR-223-3p in OA mice and IL-1β-stimulated chondrocytes. In vitro, we found that NLRP3 was a direct target of miR-223-3p, and overexpression of miR-223-3p blocked IL-1β-induced apoptosis and the inflammatory response in chondrocytes. These findings indicate that miR-223-3p/NLRP3 signaling could be used as a potential target of SIN for the treatment of OA.
Similar content being viewed by others
Abbreviations
- SIN:
-
sinomenine
- ACLT:
-
anterior cruciate ligament transection
- OA:
-
osteoarthritis
- TNF-α:
-
tumor necrosis factor-α
- IL-1β:
-
interleukin-1β
- TLR4:
-
toll-like receptor 4
- NF-κB:
-
nuclear factor kappa B
- TRAF6:
-
tumor necrosis factor receptor-associated factor 6
- NLRP3:
-
NOD-like receptor family, pyrin domain containing 3
- ASC:
-
caspase-recruitment domain
- 3′-UTR:
-
3′-untranslated regions
References
Glyn-Jones, S., A.J. Palmer, R. Agricola, A.J. Price, T.L. Vincent, H. Weinans, and A.J. Carr. 2015. Osteoarthritis. Lancet 386: 376–387.
Madej, W., A. van Caam, E.N. Blaney Davidson, P.M. van der Kraan, and P. Buma. 2014. Physiological and excessive mechanical compression of articular cartilage activates Smad2/3P signaling. Osteoarthritis and Cartilage 22: 1018–1025.
Zhang, Z., D.J. Leong, L. Xu, Z. He, A. Wang, M. Navati, S.J. Kim, D.M. Hirsh, J.A. Hardin, N.J. Cobelli, J.M. Friedman, and H.B. Sun. 2016. Curcumin slows osteoarthritis progression and relieves osteoarthritis-associated pain symptoms in a post-traumatic osteoarthritis mouse model. Arthritis Research & Therapy 18: 128.
Le Graverand-Gastineau, M.P. 2010. Disease modifying osteoarthritis drugs: Facing development challenges and choosing molecular targets. Current Drug Targets 11: 528–535.
Wang, J.H., K.S. Shih, Y.W. Wu, A.W. Wang, and C.R. Yang. 2013. Histone deacetylase inhibitors increase microRNA-146a expression and enhance negative regulation of interleukin-1beta signaling in osteoarthritis fibroblast-like synoviocytes. Osteoarthritis and Cartilage 21: 1987–1996.
Kim, H.W., Y.J. Kwon, B.W. Park, J.J. Song, Y.B. Park, and M.C. Park. 2017. Differential expressions of NOD-like receptors and their associations with inflammatory responses in rheumatoid arthritis. Clinical and Experimental Rheumatology 35: 630–637.
Gomez, R., A. Villalvilla, R. Largo, O. Gualillo, and G. Herrero-Beaumont. 2015. TLR4 signalling in osteoarthritis—finding targets for candidate DMOADs. Nature Reviews Rheumatology 11: 159–170.
Chen, C., C. Zhang, L. Cai, H. Xie, W. Hu, T. Wang, D. Lu, and H. Chen. 2017. Baicalin suppresses IL-1beta-induced expression of inflammatory cytokines via blocking NF-kappaB in human osteoarthritis chondrocytes and shows protective effect in mice osteoarthritis models. International Immunopharmacology 52: 218–226.
Feng, Z., W. Zheng, X. Li, J. Lin, C. Xie, H. Li, L. Cheng, A. Wu, and W. Ni. 2017. Cryptotanshinone protects against IL-1beta-induced inflammation in human osteoarthritis chondrocytes and ameliorates the progression of osteoarthritis in mice. International Immunopharmacology 50: 161–167.
Sun, Y., W. Liu, H. Zhang, H. Li, J. Liu, F. Zhang, T. Jiang, and S. Jiang. 2017. Curcumin prevents osteoarthritis by inhibiting the activation of inflammasome NLRP3. Journal of Interferon & Cytokine Research 37: 449–455.
Shi, H., K. Zheng, Z. Su, H. Su, M. Zhong, X. He, C. Zhou, H. Chen, Q. Xiong, and Y. Zhang. 2016. Sinomenine enhances microglia M2 polarization and attenuates inflammatory injury in intracerebral hemorrhage. Journal of Neuroimmunology 299: 28–34.
Liu, S., Q. Chen, J. Liu, X. Yang, Y. Zhang, and F. Huang. 2018. Sinomenine protects against E. coli-induced acute lung injury in mice through Nrf2-NF-kappaB pathway. Biomedicine & Pharmacotherapy 107: 696–702.
Li, X., K. Wang, Y. Ren, L. Zhang, X.J. Tang, H.M. Zhang, C.Q. Zhao, P.J. Liu, J.M. Zhang, and J.J. He. 2014. MAPK signaling mediates sinomenine hydrochloride-induced human breast cancer cell death via both reactive oxygen species-dependent and -independent pathways: An in vitro and in vivo study. Cell Death & Disease 5: e1356.
Ju, X.D., M. Deng, Y.F. Ao, C.L. Yu, J.Q. Wang, J.K. Yu, G.Q. Cui, and Y.L. Hu. 2010. Protective effect of sinomenine on cartilage degradation and chondrocytes apoptosis. Yakugaku Zasshi 130: 1053–1060.
McAllister, M.J., M. Chemaly, A.J. Eakin, D.S. Gibson, and V.E. McGilligan. 2018. NLRP3 as a potentially novel biomarker for the management of osteoarthritis. Osteoarthritis and Cartilage 26: 612–619.
Vande Walle, L., N. Van Opdenbosch, P. Jacques, A. Fossoul, E. Verheugen, P. Vogel, R. Beyaert, et al. 2014. Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis. Nature 512: 69–73.
Elliott, E.I., and F.S. Sutterwala. 2015. Initiation and perpetuation of NLRP3 inflammasome activation and assembly. Immunological Reviews 265: 35–52.
Haneklaus, M., and L.A. O'Neill. 2015. NLRP3 at the interface of metabolism and inflammation. Immunological Reviews 265: 53–62.
Bauernfeind, F., A. Rieger, F.A. Schildberg, P.A. Knolle, J.L. Schmid-Burgk, and V. Hornung. 2012. NLRP3 inflammasome activity is negatively controlled by miR-223. Journal of Immunology 189: 4175–4181.
Yang, Z., L. Zhong, R. Xian, and B. Yuan. 2015. MicroRNA-223 regulates inflammation and brain injury via feedback to NLRP3 inflammasome after intracerebral hemorrhage. Molecular Immunology 65: 267–276.
Artlett, C.M., S. Sassi-Gaha, J.L. Hope, C.A. Feghali-Bostwick, and P.D. Katsikis. 2017. Mir-155 is overexpressed in systemic sclerosis fibroblasts and is required for NLRP3 inflammasome-mediated collagen synthesis during fibrosis. Arthritis Research & Therapy 19: 144.
Zheng, X., F.C. Zhao, Y. Pang, D.Y. Li, S.C. Yao, S.S. Sun, and K.J. Guo. 2017. Downregulation of miR-221-3p contributes to IL-1beta-induced cartilage degradation by directly targeting the SDF1/CXCR4 signaling pathway. The Journal of Molecular Medicine (Berlin) 95: 615–627.
Wang, W., C. Ha, T. Lin, D. Wang, Y. Wang, and M. Gong. 2018. Celastrol attenuates pain and cartilage damage via SDF-1/CXCR4 signalling pathway in osteoarthritis rats. The Journal of Pharmacy and Pharmacology 70: 81–88.
Gong, J., Z.X. Wang, and Z.Y. Liu. 2017. miRNA1271 inhibits cell proliferation in neuroglioma by targeting fibronectin 1. Molecular Medicine Reports 16: 143–150.
Livak, K.J., and T.D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods 25: 402–408.
Song, J., I.J. Baek, C.H. Chun, and E.J. Jin. 2018. Dysregulation of the NUDT7-PGAM1 axis is responsible for chondrocyte death during osteoarthritis pathogenesis. Nature Communications 9: 3427.
Chen, C., Z. Liang, W. Huang, X. Li, F. Zhou, X. Hu, M. Han, X. Ding, and S. Xiang. 2015. Eps8 regulates cellular proliferation and migration of breast cancer. International Journal of Oncology 46: 205–214.
Afonina, I.S., Z. Zhong, M. Karin, and R. Beyaert. 2017. Limiting inflammation-the negative regulation of NF-kappaB and the NLRP3 inflammasome. Nature Immunology 18: 861–869.
Mi, B., J. Wang, Y. Liu, J. Liu, L. Hu, A.C. Panayi, G. Liu, and W. Zhou. 2018. Icariin activates autophagy via down-regulation of the NF-kappaB signaling-mediated apoptosis in chondrocytes. Frontiers in Pharmacology 9: 605.
Wang, Y., L. Li de, X.B. Zhang, Y.H. Duan, Z.H. Wu, D.S. Hao, B.S. Chen, and G.X. Qiu. 2013. Increase of TNFalpha-stimulated osteoarthritic chondrocytes apoptosis and decrease of matrix metalloproteinases 9 by NF-kappaB inhibition. Biomedical and Environmental Sciences 26: 277–283.
Cai, L., W.N. Chen, R. Li, M.M. Liu, C. Lei, C.M. Li, and Y.Y. Qiu. 2018. Acetazolamide protects rat articular chondrocytes from IL-1beta-induced apoptosis by inhibiting the activation of NF-kappaB signal pathway. Canadian Journal of Physiology and Pharmacology 96: 1104–1111.
Latz, E., T.S. Xiao, and A. Stutz. 2013. Activation and regulation of the inflammasomes. Nature Reviews. Immunology 13: 397–411.
Bonar, S.L., S.D. Brydges, J.L. Mueller, M.D. McGeough, C. Pena, D. Chen, S.K. Grimston, C.L. Hickman-Brecks, S. Ravindran, A. McAlinden, D.V. Novack, D.L. Kastner, R. Civitelli, H.M. Hoffman, and G. Mbalaviele. 2012. Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice. PLoS One 7: e35979.
Wang, C., C.X. Xu, Y. Alippe, C. Qu, J. Xiao, E. Schipani, R. Civitelli, Y. Abu-Amer, and G. Mbalaviele. 2017. Chronic inflammation triggered by the NLRP3 inflammasome in myeloid cells promotes growth plate dysplasia by mesenchymal cells. Scientific Reports 7: 4880.
Nasi, S., H.K. Ea, A. So, and N. Busso. 2017. Revisiting the role of interleukin-1 pathway in osteoarthritis: Interleukin-1alpha and -1beta, and NLRP3 Inflammasome are not involved in the pathological features of the murine menisectomy model of osteoarthritis. Frontiers in Pharmacology 8: 282.
Bougault, C., M. Gosset, X. Houard, C. Salvat, L. Godmann, T. Pap, C. Jacques, and F. Berenbaum. 2012. Stress-induced cartilage degradation does not depend on the NLRP3 inflammasome in human osteoarthritis and mouse models. Arthritis and Rheumatism 64: 3972–3981.
Clavijo-Cornejo, D., K. Martinez-Flores, K. Silva-Luna, and G.A. Martinez-Nava. 2016. The overexpression of NALP3 inflammasome in knee osteoarthritis is associated with synovial membrane prolidase and NADPH oxidase 2. Oxidative Medicine and Cellular Longevity 2016: 1472567.
Qin, D., X. Wang, Y. Li, L. Yang, R. Wang, J. Peng, K. Essandoh, X. Mu, T. Peng, Q. Han, K.J. Yu, and G.C. Fan. 2016. MicroRNA-223-5p and -3p cooperatively suppress necroptosis in ischemic/reperfused hearts. The Journal of Biological Chemistry 291: 20247–20259.
Ding, Q., L. Shen, X. Nie, B. Lu, X. Pan, Z. Su, A. Yan, R. Yan, Y. Zhou, L. Li, and J. Xu. 2018. MiR-223-3p overexpression inhibits cell proliferation and migration by regulating inflammation-associated cytokines in glioblastomas. Pathology, Research and Practice 214: 1330–1339.
Feng, Z., S. Qi, Y. Zhang, Z. Qi, L. Yan, J. Zhou, F. He, Q. Li, Y. Yang, Q. Chen, S. Xiao, Q. Li, Y. Chen, and Y. Zhang. 2017. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death & Disease 8: e3170.
Funding
This research was supported by the Medical Science Research Fund of Dalian City of China (Grant no. 1812021).
Author information
Authors and Affiliations
Contributions
Study design: HAI-CAO DON; Literature research, Data acquisition, and Data analysis: HAI-CAO DON, PEI-NAN LI, CHANG-JIAN CHEN, XIN XU, HONG ZHANG, GANG LIU, LIAN-JIE ZHENG, PENG LI; Manuscript preparation and Manuscript editing: HAI-CAO DONG, PEI-NAN LI and CHANG-JIAN CHEN; Manuscript review: T HAI-CAO DON; Cell experiments: CHANG-JIAN CHEN, XIN XU and HONG ZHANG; Animal experiments: GANG LIU, LIAN-JIE ZHENG and PENG LI. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics Approval and Consent to Participate
This study was permitted by the Ethics Committee of the Dalian Medical University (Dalian, China) on June 1st in 2017 (Approval number 20170019a).
Conflict of Interest
The authors declare that they have no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Dong, HC., Li, PN., Chen, CJ. et al. Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling. Inflammation 42, 1265–1275 (2019). https://doi.org/10.1007/s10753-019-00986-3
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10753-019-00986-3