Skip to main content

Advertisement

SpringerLink
Log in

Effects of Angelicin on Ovalbumin (OVA)-Induced Airway Inflammation in a Mouse Model of Asthma

  • ORIGINAL ARTICLE
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Angelicin, a furocoumarin found in Psoralea corylifolia L. fruit, has been reported to have anti-inflammatory activity. The purpose of this study was to determine the protective effects of angelicin on allergic asthma induced by ovalbumin (OVA) in mice. Mice were sensitized to OVA (on days 0 and 14) and challenged with OVA three times (on days 21 to 23). Angelicin (2.5, 5, 10 mg/kg) was given intraperitoneally 1 h before OVA treatment after the initial OVA sensitization. The production of IL-4, IL-5, and IL-13 in BALF and IgE in the serum were measured by ELISA. Lung histological changes were detected by using hematoxylin and eosin (H&E) stain. The results showed that angelicin significantly inhibited inflammatory cells infiltration into the lungs. Histological studies showed that angelicin significantly attenuated OVA-induced lung injury. Meanwhile, treatment of angelicin dose-dependently inhibited OVA-induced the production of IL-4, IL-5, and IL-13 in BALF and IgE in the serum. Furthermore, angelicin was found to inhibit airway hyperresponsiveness and NF-kB activation. In conclusion, our results suggested that angelicin inhibited allergic airway inflammation and hyperresponsiveness by inhibiting NF-kB activation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Bousquet, J., P.K. Jeffery, W.W. Busse, M. Johnson, and A.M. Vignola. 2000. Asthma. From bronchoconstriction to airways inflammation and remodeling. American Journal of Respiratory and Critical Care Medicine 161(5): 1720–1745.

    Article  CAS  PubMed  Google Scholar 

  2. Larché, M., D.S. Robinson, and A.B. Kay. 2003. The role of T lymphocytes in the pathogenesis of asthma. Journal of Allergy and Clinical Immunology 111(3): 450–463.

    Article  CAS  PubMed  Google Scholar 

  3. Romagnani, S. 2000. The role of lymphocytes in allergic disease. Journal of Allergy and Clinical Immunology 105(3): 399–408.

    Article  CAS  PubMed  Google Scholar 

  4. Bradding, P., J. Roberts, K. Britten, S. Montefort, R. Djukanovic, R. Mueller, C. Heusser, P. Howarth, and S. Holgate. 1994. Interleukin-4,-5, and-6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines. American Journal of Respiratory Cell and Molecular Biology 10(5): 471–480.

    Article  CAS  PubMed  Google Scholar 

  5. Advenier, C., V. Lagente, and E. Boichot. 1997. The role of tachykinin receptor antagonists in the prevention of bronchial hyperresponsiveness, airway inflammation and cough. European Respiratory Journal 10(8): 1892–1906.

    Article  CAS  PubMed  Google Scholar 

  6. Holgate, S., T. Casale, S. Wenzel, J. Bousquet, Y. Deniz, and C. Reisner. 2005. The anti-inflammatory effects of omalizumab confirm the central role of IgE in allergic inflammation. Journal of Allergy and Clinical Immunology 115(3): 459–465.

    Article  CAS  PubMed  Google Scholar 

  7. Cho, H.J., S.G. Jeong, J.E. Park, J.A. Han, H.R. Kang, D. Lee, and M.J. Song. 2013. Antiviral activity of angelicin against gammaherpesviruses. Antiviral Research 100(1): 75–83.

    Article  CAS  PubMed  Google Scholar 

  8. Liu, F., G.-Q. Sun, H.-Y. Gao, R.-S. Li, L.-W. Soromou, N. Chen, Y.-H. Deng, and H.-H. Feng. 2013. Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-kB pathways. Journal of Surgical Research 185(1): 300–309.

    Article  CAS  PubMed  Google Scholar 

  9. Wang, C.C., J.E. Lai, L.G. Chen, K.Y. Yen, and L.L. Yang. 2000. Inducible nitric oxide synthase inhibitors of Chinese herbs. Part 2: naturally occurring furanocoumarins. Bioorganic & Medicinal Chemistry 8(12): 2701–2707.

    Article  CAS  Google Scholar 

  10. Zosky, G.R., and P.D. Sly. 2007. Animal models of asthma. Clinical and Experimental Allergy 37(7): 973–988.

    Article  CAS  PubMed  Google Scholar 

  11. Ackerman, K.G., H. Huang, H. Grasemann, C. Puma, J.B. Singer, A.E. Hill, E. Lander, J.H. Nadeau, G.A. Churchill, J.M. Drazen, and D.R. Beier. 2005. Interacting genetic loci cause airway hyperresponsiveness. Physiological Genomics 21(1): 105–111.

    Article  CAS  PubMed  Google Scholar 

  12. Song, C., L. Luo, Z. Lei, B. Li, Z. Liang, G. Liu, D. Li, G. Zhang, B. Huang, and Z.-H. Feng. 2008. IL-17-producing alveolar macrophages mediate allergic lung inflammation related to asthma. The Journal of Immunology 181(9): 6117–6124.

    Article  CAS  PubMed  Google Scholar 

  13. Kapp, A. 1993. The role of eosinophils in the pathogenesis of atopic dermatitis-eosinophil granule proteins as markers of disease activity. Allergy 48(1): 1–5.

    Article  CAS  PubMed  Google Scholar 

  14. Hamid, Q., M.K. Tulic, M.C. Liu, and R. Moqbel. 2003. Inflammatory cells in asthma: mechanisms and implications for therapy. Journal of Allergy and Clinical Immunology 111(1): S5–S17.

    Article  CAS  PubMed  Google Scholar 

  15. Chung, K. 1986. Role of inflammation in the hyperreactivity of the airways in asthma. Thorax 41(9): 657–662.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Wakashin, H., K. Hirose, Y. Maezawa, S.-I. Kagami, A. Suto, N. Watanabe, Y. Saito, M. Hatano, T. Tokuhisa, and Y. Iwakura. 2008. IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. American Journal of Respiratory and Critical Care Medicine 178(10): 1023–1032.

    Article  CAS  PubMed  Google Scholar 

  17. Renauld, J.-C. 2001. New insights into the role of cytokines in asthma. Journal of Clinical Pathology 54(8): 577–589.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Lebman, D.A., and R.L. Coffman. 1988. Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures. The Journal of Experimental Medicine 168(3): 853–862.

    Article  CAS  PubMed  Google Scholar 

  19. Carballido, J.M., D. Schols, R. Namikawa, S. Zurawski, G. Zurawski, M.-G. Roncarolo, and J. De Vries. 1995. IL-4 induces human B cell maturation and IgE synthesis in SCID-hu mice. Inhibition of ongoing IgE production by in vivo treatment with an IL-4/IL-13 receptor antagonist. The Journal of Immunology 155(9): 4162–4170.

    CAS  PubMed  Google Scholar 

  20. O’Byrne, P.M., M.D. Inman, and K. Parameswaran. 2001. The trials and tribulations of IL-5, eosinophils, and allergic asthma. Journal of Allergy and Clinical Immunology 108(4): 503–508.

    Article  PubMed  Google Scholar 

  21. Huffnagle, G.B., M.B. Boyd, N.E. Street, and M.F. Lipscomb. 1998. IL-5 is required for eosinophil recruitment, crystal deposition, and mononuclear cell recruitment during a pulmonary Cryptococcus neoformans infection in genetically susceptible mice (C57BL/6). The Journal of Immunology 160(5): 2393–2400.

    CAS  PubMed  Google Scholar 

  22. Kuperman, D.A., X. Huang, L.L. Koth, G.H. Chang, G.M. Dolganov, Z. Zhu, J.A. Elias, D. Sheppard, and D.J. Erle. 2002. Direct effects of interleukin-13 on epithelial cells cause airway hyperreactivity and mucus overproduction in asthma. Nature Medicine 8(8): 885–889.

    CAS  PubMed  Google Scholar 

  23. Yang, G., A. Volk, T. Petley, E. Emmell, J. Giles-Komar, X. Shang, J. Li, A.M. Das, D. Shealy, and D.E. Griswold. 2004. Anti-IL-13 monoclonal antibody inhibits airway hyperresponsiveness, inflammation and airway remodeling. Cytokine 28(6): 224–232.

    Article  CAS  PubMed  Google Scholar 

  24. Wills‐Karp, M. 2004. Interleukin-13 in asthma pathogenesis. Immunological Reviews 202(1): 175–190.

    Article  PubMed  Google Scholar 

  25. Wills-Karp, M., J. Luyimbazi, X. Xu, B. Schofield, T.Y. Neben, C.L. Karp, and D.D. Donaldson. 1998. Interleukin-13: central mediator of allergic asthma. Science 282(5397): 2258–2261.

    Article  CAS  PubMed  Google Scholar 

  26. Webb, D.C., A.N. McKenzie, A.M. Koskinen, M. Yang, J. Mattes, and P.S. Foster. 2000. Integrated signals between IL-13, IL-4, and IL-5 regulate airways hyperreactivity. The Journal of Immunology 165(1): 108–113.

    Article  CAS  PubMed  Google Scholar 

  27. Huang, C.S., A.H. Lin, T.C. Yang, K.L. Liu, H.W. Chen, and C.K. Lii. 2015. Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFkappaB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells. Biochemical Pharmacology 93(3): 352–361.

    Article  CAS  PubMed  Google Scholar 

  28. Rai, A., S. Kapoor, S. Singh, B.P. Chatterji, and D. Panda. 2015. Transcription factor NF-kappaB associates with microtubules and stimulates apoptosis in response to suppression of microtubule dynamics in MCF-7 cells. Biochemical Pharmacology 93(3): 277–289.

    Article  CAS  PubMed  Google Scholar 

  29. Zhao, Z.Z., X.F. Tang, X.H. Zhao, M.H. Zhang, W.J. Zhang, S.H. Hou, W.F. Yuan, H.F. Zhang, L.J. Shi, H. Jia, L. Liang, Z. Lai, J.F. Gao, K.Y. Zhang, L. Fu, and W. Chen. 2014. Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-kappa B activation. Biochemical Pharmacology 90(1): 73–87.

    Article  CAS  PubMed  Google Scholar 

  30. Bureau, F., S. Delhalle, G. Bonizzi, L. Fiévez, S. Dogné, N. Kirschvink, A. Vanderplasschen, M.-P. Merville, V. Bours, and P. Lekeux. 2000. Mechanisms of persistent NF-kB activity in the bronchi of an animal model of asthma. The Journal of Immunology 165(10): 5822–5830.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The project received the financial support from the Science and Technology Bureau of Wenzhou (Y20140253), Zhejiang Province, China.

Author information

Authors and Affiliations

  1. Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China

    Da-zhen Wei, Xian-yang Guo, Meng-xiang Lin, Yu-qiang Gong & Bin-yu Ying

  2. Department of Neurology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China

    Li-na Lin

  3. Department of Cardiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China

    Ming-yuan Huang

Authors
  1. Da-zhen Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xian-yang Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li-na Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Meng-xiang Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yu-qiang Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bin-yu Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ming-yuan Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ming-yuan Huang.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wei, Dz., Guo, Xy., Lin, Ln. et al. Effects of Angelicin on Ovalbumin (OVA)-Induced Airway Inflammation in a Mouse Model of Asthma. Inflammation 39, 1876–1882 (2016). https://doi.org/10.1007/s10753-016-0423-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-016-0423-2

KEY WORDS

Search

Navigation