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The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats

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Abstract

The main causes of spinal cord ischemia are a variety of vascular pathologies causing acute arterial occlusions. We investigated neuroprotective effects of coumaric acid on spinal cord ischemia injury in rats. Rats were divided randomly into four groups of eight animals as follows: control, ischemia, ischemia + coumaric acid, and ischemia + methylprednisolone. In the control group, only a laparotomy was performed. In all other groups, the spinal cord ischemia was performed by the infrarenal aorta cross-clamping model. Levels of malondialdehyde and nuclear respiratory factor 1 were analyzed, as were the activity of superoxide dismutase. Histopathological and immunohistochemical evaluations were performed. Neurological evaluation was performed with the Tarlov scoring system. The ischemia + coumaric acid group was compared with the ischemia group, and a significant decrease in malondialdehyde and levels was observed. Nuclear respiratory factor 1 level and superoxide dismutase activity of the ischemia + coumaric acid group were significantly higher than in the ischemia group. In histopathological samples, the ischemia + coumaric acid group is compared with the ischemia group, and there was a significant increase in numbers of normal neurons. In immunohistochemical staining, hypoxia-inducible factor-1α and NF-kappa B immunopositive neurons were significantly decreased in the ischemia + coumaric acid group compared with that in the ischemia group. The neurological deficit scores of the ischemia + coumaric acid group were significantly higher than the ischemia group at 24 h. Our results revealed for the first time that coumaric acid exhibits meaningful neuroprotective activity following ischemia-reperfusion injury of the spinal cord.

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Conflict of Interest

The authors declare that they have no competing interests and financial disclosure.

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Authors and Affiliations

  1. Department of Neurosurgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Mustafa Guven, Adem Bozkurt Aras, Tarik Akman & Murat Cosar

  2. Department of Medical Biochemistry, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Muserref Hilal Sehitoglu

  3. Department of Histology & Embryology, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey

    Yasemin Yuksel

  4. Department of Neurosurgery, Faculty of Medicine, Medipol University, Istanbul, Turkey

    Mehmet Tokmak

  5. Department of Orthopaedics, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Umut Hatay Golge

  6. Department of Neurosurgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey

    Ergun Karavelioglu

  7. Department of Neurosurgery, Ataturk Education and Research Hospital, Ankara, Turkey

    Ercan Bal

Authors
  1. Mustafa Guven
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  2. Muserref Hilal Sehitoglu
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  3. Yasemin Yuksel
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  4. Mehmet Tokmak
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  5. Adem Bozkurt Aras
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  6. Tarik Akman
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  7. Umut Hatay Golge
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  8. Ergun Karavelioglu
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  9. Ercan Bal
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  10. Murat Cosar
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Correspondence to Mustafa Guven.

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Guven, M., Sehitoglu, M.H., Yuksel, Y. et al. The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats. Inflammation 38, 1986–1995 (2015). https://doi.org/10.1007/s10753-015-0179-0

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  • DOI: https://doi.org/10.1007/s10753-015-0179-0

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