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Oxymatrine Lightened the Inflammatory Response of LPS-Induced Mastitis in Mice Through Affecting NF-κB and MAPKs Signaling Pathways

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Abstract

Mastitis, an inflammatory reaction of the mammary gland, is recognized as one of the most costly diseases in dairy cattle. Oxymatrine, one of the alkaloids extracted from Chinese herb Sophora flavescens Ait, has been reported to have many biological activities, such as anti-inflammatory, anti-virus, and anti-hepatic fibrosis properties. The aim of this study was to investigate the protective effect and the anti-inflammatory mechanism of oxymatrine on lipopolysaccharide (LPS)-induced mastitis in mice. The mouse mastitis was induced by 10 μg of LPS for 24 h. Oxymatrine was intraperitoneally administered with the dose of 30, 60, and 120 mg/kg 1 h before and 12 h after LPS induction. The results showed that oxymatrine significantly attenuated the damage of the mammary gland induced by LPS. Oxymatrine inhibited the phosphorylation of NF-κB p65 and IκB in NF-κB signal pathway and reduced the phosphorylation of p38, ERK, and JNK in mitogen-activated protein kinase (MAPKs) signal pathway. The results showed that oxymatrine had a protective effect on LPS-induced mastitis, and the anti-inflammatory mechanism of oxymatrine was related to the inhibition of NF-κB and MAPKs signal pathways.

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Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (No. 31272622, 31201926).

Conflict of Interests

The authors declare that they have no conflicts of interests.

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Authors and Affiliations

  1. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, People’s Republic of China

    Zhengtao Yang, Zhanqing Yang, Ershun Zhou, Zhengkai Wei, Zhicheng Liu, Yongguo Cao & Naisheng Zhang

  2. Research Academy of Animal Husbandry and Veterinary Medicine Sciences of Jilin Province, Changchun, Jilin, 130062, People’s Republic of China

    Ronglan Yin

  3. Department of Gynecology, The First Hospital of Jilin University, Jilin University, Changchun, Jilin, 130061, People’s Republic of China

    Yunfeng Cong

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  1. Zhengtao Yang
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Correspondence to Naisheng Zhang.

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Yang, Z., Yin, R., Cong, Y. et al. Oxymatrine Lightened the Inflammatory Response of LPS-Induced Mastitis in Mice Through Affecting NF-κB and MAPKs Signaling Pathways. Inflammation 37, 2047–2055 (2014). https://doi.org/10.1007/s10753-014-9937-7

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