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Phenyl-β-d-Glucopyranoside Exhibits Anti-inflammatory Activity in Lipopolysaccharide-Activated RAW 264.7 Cells

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Abstract

Phenyl-β-d-glucopyranoside is a component of Phellodendron amurense with anti-cancer and anti-inflammatory activities. In the present study, we investigated the role of phenyl-β-d-glucopyranoside in inflammation using lipopolysaccharide (LPS)-stimulated murine Raw 264.7 macrophages. Phenyl-β-d-glucopyranoside not only inhibited nitric oxide (NO) production but also significantly inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) without inducing cytotoxicity. Phenyl-β-d-glucopyranoside also attenuated proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and other inflammation-related genes, such as IL-6 in a concentration-dependent manner. Furthermore, phenyl-β-d-glucopyranoside abolished increased adhesion, ninjurin 1 (Ninj1) expression, and matrix metalloproteinase (MMP) activity induced by endotoxin treatment. Finally, phenyl-β-d-glucopyranoside inhibited the nuclear translocation of nuclear factor-κB (NF-κB), which is one of the most important transcription factors involved in the inflammatory process. Taken together, phenyl-β-d-glucopyranoside may be beneficial for the prevention and treatment of anti-inflammatory diseases.

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Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean Government, the Ministry of Science, ICT and Future Planning (2013R1A1A1059709).

Conflict of Interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. College of Pharmacy, Inje University, Gimhae, Korea

    Su Jung Hwang & Hyo-Jong Lee

  2. u-Healthcare & Anti-aging Research Center (u-HARC), Inje University, Gimhae, Korea

    Hyo-Jong Lee

  3. Biohealth Products Research Center (BPRC), Inje University, Gimhae, Korea

    Hyo-Jong Lee

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  1. Su Jung Hwang
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Correspondence to Hyo-Jong Lee.

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Hwang, S.J., Lee, HJ. Phenyl-β-d-Glucopyranoside Exhibits Anti-inflammatory Activity in Lipopolysaccharide-Activated RAW 264.7 Cells. Inflammation 38, 1071–1079 (2015). https://doi.org/10.1007/s10753-014-0072-2

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  • DOI: https://doi.org/10.1007/s10753-014-0072-2

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