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Protective Effect of Kaempferol on LPS plus ATP-Induced Inflammatory Response in Cardiac Fibroblasts

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Abstract

Inflammatory response is an important mechanism in the pathogenesis of cardiovascular diseases. Cardiac fibroblasts play a crucial role in cardiac inflammation and might become a potential therapeutic target in cardiovascular diseases. Kaempferol, a flavonoid commonly existing in many edible fruits, vegetables, and Chinese herbs, is well known to possess anti-inflammatory property and thus has a therapeutic potential for the treatment of inflammatory diseases. To date, the effect of kaempferol on cardiac fibroblasts inflammation is unknown. In this study, we investigated the anti-inflammatory effect of kaempferol on lipopolysaccharide (LPS) plus ATP-induced cardiac fibroblasts and explored the underlying mechanisms. Our results showed that kaempferol at concentrations of 12.5 and 25 μg/mL significantly suppressed the release of TNF-α, IL-1β, IL-6, and IL-18 and inhibited activation of NF-κB and Akt in LPS plus ATP-induced cardiac fibroblasts. These findings suggest that kaempferol attenuates cardiac fibroblast inflammation through suppression of activation of NF-κB and Akt.

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (Grant Nos. 81073085; 81001662) and the National Science and Technology Major Project of China (Grant Nos. 2012zx09301002-004-002; 2012zx09103201-049).

Conflict of Interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Xi-lan Tang, Jian-xun Liu, Peng Li, Lei Li, Jin-cai Hou, Yong-qiu Zheng, Cheng-ren Lin & Jun-guo Ren

  2. Key Laboratory of Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China

    Xi-lan Tang & Wei Dong

  3. Experimental Research Center, Xiyuan Hospital, No. 1 Xicaochang, Beijing, Haidian District, 100091, China

    Jian-xun Liu

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  1. Xi-lan Tang
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Correspondence to Jian-xun Liu.

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ESM 1

Cardiac fibroblasts were stimulated with LPS (100 ng/mL, 3 h) followed by ATP (5 mmol/L) during the indicated time. The IL-1β level of the culture supernatants was measured by ELISA kit. Data are shown as means ± SEM (n = 3) (TIFF 472 kb).

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Tang, Xl., Liu, Jx., Dong, W. et al. Protective Effect of Kaempferol on LPS plus ATP-Induced Inflammatory Response in Cardiac Fibroblasts. Inflammation 38, 94–101 (2015). https://doi.org/10.1007/s10753-014-0011-2

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