Skip to main content

Advertisement

SpringerLink
Log in

Interleukin (IL)-10 Gene Polymorphisms Are Associated with Type 2 Diabetes With and Without Nephropathy: A Study of Patients from the Southeast Region of Iran

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The impact of several environmental and genetic factors on diabetes and its complications is well documented. It has also been established that cytokines play a key role in the regulation of immune responses which have been shown to be important in the pathogenesis of diabetes. Studies showed that single-nucleotide polymorphisms within the −592 region of interleukin-10 (IL-10) are associated with the regulation of its expression. In this study, we aimed to find polymorphisms of this region that may be associated to type 2 diabetic (T2D) patients with and without nephropathy. In this study, peripheral blood samples were collected from 100 T2D patients without nephropathy, 100 T2D patients with nephropathy, and 100 healthy controls. DNA was extracted, and a polymerase chain reaction-restriction fragment length polymorphism technique was performed to examine the polymorphisms within the −592 region of the IL-10 gene. Our results showed a significant difference between the genotypes and alleles of the −592 region of IL-10 in nephropathic and non-nephropathic patients in comparison to the healthy controls. The differences between the two patient groups in relation to genotypes and alleles were not significant. Results of this study suggest that the functional gene polymorphism of IL-10 reported here may play an important role in the pathogenesis of diabetes, but it seems that these polymorphisms do not have an effect on the nephropathic complications of the disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Steyn, N.P., E.V. Lambert, and H. Tabana. 2008. Nutrition interventions for the prevention of type 2 diabetes. Proc Nutr Soc 10: 1–16.

    Google Scholar 

  2. Zimmet, P., K.G. Alberti, and J. Shaw. 2001. Global and societal implications of the diabetes epidemic. Nature 414: 782–787.

    Article  PubMed  CAS  Google Scholar 

  3. Nathanson, D., and T. Nystrom. 2008. Hypoglycemic pharmacological treatment of type 2 diabetes: targeting the endothelium. Mol Cell Endocrinol 21: 21.

    Google Scholar 

  4. Cruz, M., C. Maldonado-Bernal, R. Mondragon-Gonzalez, R. Sanchez-Barrera, N.H. Wacher, G. Carvajal-Sandoval, and J. Kumate. 2008. Glycine treatment decreases proinflammatory cytokines and increases interferon-gamma in patients with type 2 diabetes. J Endocrinol Invest 31: 694–699.

    PubMed  CAS  Google Scholar 

  5. Giulietti, A., E. van Etten, L. Overbergh, K. Stoffels, R. Bouillon, and C. Mathieu. 2007. Monocytes from type 2 diabetic patients have a pro-inflammatory profile. 1,25-Dihydroxyvitamin D(3) works as anti-inflammatory. Diabetes Res Clin Pract 77: 47–57.

    Article  PubMed  CAS  Google Scholar 

  6. Tsiavou, A., D. Degiannis, E. Hatziagelaki, K. Koniavitou, and S.A. Raptis. 2004. Intracellular IFN-gamma production and IL-12 serum levels in latent autoimmune diabetes of adults (LADA) and in type 2 diabetes. J Interferon Cytokine Res 24: 381–387.

    Article  PubMed  CAS  Google Scholar 

  7. Arababadi, M.K., R. Nosratabadi, G. Hassanshahi, N. Yaghini, V. Pooladvand, A. Shamsizadeh, H. Hakimi, and R. Derakhshan. 2009. Nephropathic complication of type-2 diabetes is following pattern of autoimmune diseases? Diabetes Res Clin Pract 87: 33–37.

    Article  PubMed  Google Scholar 

  8. Skopinski, P., E. Rogala, B. Duda-Krol, A. Lipinska, E. Sommer, J. Chorostowska-Wynimko, J. Szaflik, I. Partyka, and E. Skopinska-Rozewska. 2005. Increased interleukin-18 content and angiogenic activity of sera from diabetic (type 2) patients with background retinopathy. J Diabetes Complications 19: 335–338.

    Article  PubMed  Google Scholar 

  9. Pickup, J.C., G.D. Chusney, S.M. Thomas, and D. Burt. 2000. Plasma interleukin-6, tumour necrosis factor alpha and blood cytokine production in type 2 diabetes. Life Sci 67: 291–300.

    Article  PubMed  CAS  Google Scholar 

  10. Suppiah, V., A. Goris, I. Alloza, S. Heggarty, B. Dubois, H. Carton, A. Antiguedad, M. Mendibe, G. McDonnell, A. Droogan, S. Hawkins, C. Graham, and K. Vandenbroeck. 2005. Polymorphisms in the interleukin-4 and IL-4 receptor genes and multiple sclerosis: a study in Spanish-Basque, Northern Irish and Belgian populations. Int J Immunogenet 32: 383–388.

    Article  PubMed  CAS  Google Scholar 

  11. Lopez, P., C. Gutierrez, and A. Suarez. 2010. IL-10 and TNFalpha genotypes in SLE. J Biomed Biotechnol 2010: 838390.

    Article  PubMed  Google Scholar 

  12. Chen, J., and X. Liu. 2009. The role of interferon gamma in regulation of CD4+ T-cells and its clinical implications. Cell Immunol 254: 85–90.

    Article  PubMed  CAS  Google Scholar 

  13. Amirzargar, M., M. Yavangi, A. Basiri, S.H. Moghadam, F. Khosravi, G. Solgi, M. Gholiaf, F. Khoshkho, F. Dadaras, M. Mahmmodi, B. Ansaripour, A. Amirzargar, and B. Nikbin. 2007. Genetic association of interleukin-4, interleukin-10, and transforming growth factor-beta gene polymorphism with allograft function in renal transplant patients. Transplant Proc 39: 954–957.

    Article  PubMed  CAS  Google Scholar 

  14. Wang, X.H., W. Zhao, S.G. Liu, and X.P. Feng. 2009. Correlation of IL-4 and IL-13 gene polymorphisms with asthma and total serum IgE levels. Zhonghua Jie He He Hu Xi Za Zhi 32: 161–164.

    PubMed  Google Scholar 

  15. Luczynski, W., A. Stasiak-Barmuta, A. Juchniewicz, N. Wawrusiewicz-Kurylonek, E. Ilendo, J. Kos, A. Kretowski, M. Gorska, L. Chyczewski, and A. Bossowski. 2010. The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes. Folia Histochem Cytobiol 48: 93–100.

    Article  PubMed  Google Scholar 

  16. Sanjabi, S., L.A. Zenewicz, M. Kamanaka, and R.A. Flavell. 2009. Anti-inflammatory and pro-inflammatory roles of TGF-beta, IL-10, and IL-22 in immunity and autoimmunity. Curr Opin Pharmacol 9: 447–453.

    Article  PubMed  CAS  Google Scholar 

  17. Karjalainen, J., J. Hulkkonen, M.M. Nieminen, H. Huhtala, A. Aromaa, T. Klaukka, and M. Hurme. 2003. Interleukin-10 gene promoter region polymorphism is associated with eosinophil count and circulating immunoglobulin E in adult asthma. Clin Exp Allergy 33: 78–83.

    Article  PubMed  CAS  Google Scholar 

  18. Momeni, A., S. Shahidi, S. Seirafian, S. Taheri, and S. Kheiri. 2011. Effect of allopurinol in decreasing proteinuria in type 2 diabetic patients. Iran J Kidney Dis 4: 128–132.

    Google Scholar 

  19. Brown, S. 2010. Re: estimation of glomerular filtration rate with creatinine-based versus cystatin C-based equations in kidney transplant recipients. Iran J Kidney Dis 4: 169–170.

    PubMed  Google Scholar 

  20. Enriquez, R., A.E. Sirvent, E. Andrada, C. Escolano, J.C. Rodriguez, I. Millan, F. Gutierrez, and F. Amoros. 2010. Cryoglobulinemic glomerulonephritis in chronic hepatitis B infection. Ren Fail 32: 518–522.

    Article  PubMed  CAS  Google Scholar 

  21. Hrbacek, J., P. Konopasek, V. Eis, E. Hamsikova, R. Tachezy, J. Pokorny, K. El-Balouly, M. Urban, and J. Heracek. 2011. Urologic complications of HIV infection. Cas Lek Cesk 149: 115–119.

    Google Scholar 

  22. King, G.L. 2008. The role of inflammatory cytokines in diabetes and its complications. J Periodontol 79: 1527–1534.

    Article  PubMed  CAS  Google Scholar 

  23. Murugan, A.T., and G. Sharma. 2008. Obesity and respiratory diseases. Chron Respir Dis 5: 233–242.

    Article  PubMed  CAS  Google Scholar 

  24. Fagard, R.H., and P.M. Nilsson. 2009. Smoking and diabetes—the double health hazard! Prim Care Diabetes 3: 205–209.

    Article  PubMed  Google Scholar 

  25. Cignarelli, M., O. Lamacchia, S. Di Paolo, and L. Gesualdo. 2008. Cigarette smoking and kidney dysfunction in diabetes mellitus. J Nephrol 21: 180–189.

    PubMed  Google Scholar 

  26. Arababadi, M.K., N. Naghavi, G. Hassanshahi, and M. Mahmoodi. 2009. Is CCR5-Delta32 mutation associated with diabetic nephropathy in type 2 diabetes? Ann Saudi Med 29: 413.

    Article  PubMed  Google Scholar 

  27. Arababadi, M.K., A.A. Pourfathollah, A. Jafarzadeh, and G. Hassanshahi. 2010. Serum levels of interleukin (IL)-10 and IL-17A in occult HBV infected south-east Iranian patients. Hepat Mon 10(1): 31–35.

    PubMed  Google Scholar 

  28. Arababadi, M.K., A.A. Pourfathollah, S. Daneshmandi, G. Hassanshahi, E.R. Zarandi, A. Shamsizadeh, M.A. Rezaei, and S. Eigder. 2009. Evaluation of relation between IL-4 and IFN-g polymorphisms and type 2 diabetes. Iran J Bas Med Scie 12(2): 100–104.

    CAS  Google Scholar 

  29. Ezzidi, I., N. Mtiraoui, M. Kacem, S.G. Mallat, M.B. Mohamed, M. Chaieb, T. Mahjoub, and W.Y. Almawi. 2009. Interleukin-10-592C/A, -819C/T and -1082A/G promoter variants affect the susceptibility to nephropathy in Tunisian type 2 diabetes (T2DM) patients. Clin Endocrinol (Oxf) 70: 401–407.

    Article  CAS  Google Scholar 

  30. Scarpelli, D., M. Cardellini, F. Andreozzi, E. Laratta, M.L. Hribal, M.A. Marini, V. Tassi, R. Lauro, F. Perticone, and G. Sesti. 2006. Variants of the interleukin-10 promoter gene are associated with obesity and insulin resistance but not type 2 diabetes in Caucasian Italian subjects. Diabetes 55: 1529–1533.

    Article  PubMed  CAS  Google Scholar 

  31. Chang, Y.H., C.N. Huang, C.Y. Wu, and M.Y. Shiau. 2005. Association of interleukin-10 A-592C and T-819C polymorphisms with type 2 diabetes mellitus. Hum Immunol 66: 1258–1263.

    Article  PubMed  CAS  Google Scholar 

  32. Mtiraoui, N., I. Ezzidi, M. Kacem, M. Ben Hadj Mohamed, M. Chaieb, A.B. Haj Jilani, T. Mahjoub, and W.Y. Almawi. 2009. Predictive value of interleukin-10 promoter genotypes and haplotypes in determining the susceptibility to nephropathy in type 2 diabetes patients. Diabetes Metab Res Rev 25: 57–63.

    Article  PubMed  CAS  Google Scholar 

  33. Kolla, V.K., G. Madhavi, B. Pulla Reddy, B.M. Srikanth Babu, J. Yashovanthi, V.L. Valluri, J. Ramesh, and J. Akka. 2009. Association of tumor necrosis factor alpha, interferon gamma and interleukin 10 gene polymorphisms with peripheral neuropathy in South Indian patients with type 2 diabetes. Cytokine 47: 173–177.

    Article  PubMed  CAS  Google Scholar 

  34. Arababadi, M.K. 2010. Interleukin-4 gene polymorphisms in type 2 diabetic patients with nephropathy. Iran J Kidney Dis 4: 302–306.

    Google Scholar 

Download references

Acknowledgements

The authors of this article would like to take this opportunity to thank all the diabetic patients and healthy controls who contributed to this research; your dedication is deeply appreciated. We would also like to thank all the staff working at the diabetes clinic in the Ali Ebn-Abitaleb Hospital for their warm cooperation and technical assistance. This project was supported by a grant from the Rafsanjan University of Medical Sciences.

Author information

Authors and Affiliations

  1. Department of Microbiology, Hematology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Kazemi Arababadi

  2. Molecular-Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Kazemi Arababadi, Mohammad Reza Mirzaei, Gholamhossein Hassanshahi & Vajihe Akbarpour Salehabadi

  3. Infectious and Tropical Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Kazemi Arababadi & Behzad Nasiri Ahmadabadi

  4. Ali-Ebne Abitaleb Hospital, Rafsanjan University of Medical Science, Rafsanjan, Iran

    Seyed Mohammad Ali Sajadi & Reza Derakhshan

  5. School of Biomolecular and Physical Science, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, QLD, Australia

    Derek Kennedy

  6. Department of Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Reza Mirzaei

Authors
  1. Mohammad Kazemi Arababadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohammad Reza Mirzaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Seyed Mohammad Ali Sajadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gholamhossein Hassanshahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Behzad Nasiri Ahmadabadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vajihe Akbarpour Salehabadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Reza Derakhshan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Derek Kennedy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohammad Reza Mirzaei.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kazemi Arababadi, M., Reza Mirzaei, M., Ali Sajadi, S.M. et al. Interleukin (IL)-10 Gene Polymorphisms Are Associated with Type 2 Diabetes With and Without Nephropathy: A Study of Patients from the Southeast Region of Iran. Inflammation 35, 797–802 (2012). https://doi.org/10.1007/s10753-011-9376-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-011-9376-7

KEY WORDS

Search

Navigation