Abstract
Tooth formation is accomplished under strict genetic control procedures. Therefore, exploring the gene network system of tooth development has a very positive practical significance for the study of tooth tissue regeneration and the prevention and treatment of tooth abnormalities. Early bell stage is the initial phase of odontoblast formation and dentin matrix deposition in the process of tooth development. Through RNA sequencing and differential gene analysis of the rat tooth germ samples at cap stage and early bell stage, we found that the bile secretion pathway was the most significant difference signal pathway during the development between cap stage and bell stage, which mainly included ABCC3, AQP4, SLC10A1, SLC2A1, SLC4A4, ADCY5, AQP9, CFTR, ATP1A2, ATP1B1 and ATP1A1, totally 11genes. Immunostaining revealed that SLC2A1, SLC4A4, ADCY5 and ATP1B1were mainly expressed in epithelium in bud stage and inner and outer enamel epithelium during the embryonic phase. In the postnatal 1 and postnatal 7, SLC2A1, SLC4A4 and ABCC3 were highly expressed in ameloblasts and odontoblasts while ADCY5, ATP1B1 and SLC10A1was expressed moderately only in odontoblasts. This finding illustrated that the bile secretion pathway related genes may participate in the development of tooth germ.
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Acknowledgements
We are very grateful for the help from Institute of Digestive Diseases of Fudan University. We thank Prof. Jun Zhang for his technical support for our research work. This work was supported, in whole or in part by the National Basic Research Program (973 Program, No. 2013CB967501), the National Natural Science Foundation of China (Nos. 81570965, 81070825, 81170951).
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JY: conception and design, collection and assembly of data, and data analysis and manuscript writing; XL: data analysis and interpretation; SL and SZ: conception and design, manuscript editing, and final approval of manuscript.
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Yang, J., Lu, X., Liu, S. et al. The involvement of genes related to bile secretion pathway in rat tooth germ development. J Mol Hist 51, 99–107 (2020). https://doi.org/10.1007/s10735-020-09861-0
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DOI: https://doi.org/10.1007/s10735-020-09861-0