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Tissue expression and cellular localization of phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA in male mice

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Abstract

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is an ubiquitous antioxidant enzyme, but the exact expression pattern in mammalian tissues is still unknown. The expression and cellular localization of PHGPx mRNA were examined in male mice using real time-polymerase chain reaction and in situ hybridization techniques. The rank order of PHGPx mRNA expression across tissues exhibiting substantial levels of expression was:testes ≫ heart  >  cerebrum ≥ ileum  >  stomach = liver = jejunum ≥ epididymis. In testes, PHGPx mRNA was highly expressed in spermiogenic cells and Leydig cells. The signal was also expressed in the molecular layer, Purkinje cell layer, and white matter of cerebellum, the pituicytes of neurohypophysis, the parafollicular cells and follicular basement membrane of thyroid, the exocrine portion of pancreas, the tubular epithelium of kidney, the smooth muscle cells of arteries, and the red pulp of spleen. In the gastrointestinal tract, PHGPx mRNA expression was mainly observed in the keratinized surface epithelium of forestomach, the submucosal glands and serosa layers, and further the Paneth cells of intestines. PHGPx mRNA appeared to be ubiquitously expressed in the parenchyma of heart, liver, and lung. These results indicate that PHGPx exhibits a cell- and tissue-specific expression pattern in mice.

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Acknowledgment

This work was supported by the Korea Research Foundation Grant funded by the Korea Government [MOEHRD, Basic Research Promotion Fund (KRF-2005-005-J15002)].

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Authors and Affiliations

  1. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, 361-763, Korea

    In-Jeoung Baek, Jung-Min Yon, Se-Ra Lee, Yan Jin, Sang Soep Nahm, Jong-Koo Kang, Beom Jun Lee, Young Won Yun & Sang-Yoon Nam

  2. Safety Research Center, Korea Testing and Research Institute for Chemical Industry, Gimpo, 415-871, Korea

    Dong-Suk Seo

  3. Department of Bio-industry, Seowon University, Cheongju, 361-742, Korea

    Jae-Hwang Jeong

  4. Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Young-Kug Choo

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  1. In-Jeoung Baek
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Correspondence to Sang-Yoon Nam.

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Baek, IJ., Seo, DS., Yon, JM. et al. Tissue expression and cellular localization of phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA in male mice. J Mol Hist 38, 237–244 (2007). https://doi.org/10.1007/s10735-007-9092-7

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  • DOI: https://doi.org/10.1007/s10735-007-9092-7

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