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Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis

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Abstract

In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bovine testis by means of immunohistochemical method. Our results demonstrated that EGF and EGFR were localized solely to the bovine testicular germ cells (spermatogonia, spermatocytes, and round spermatids). In contrast, the somatic testicular cells (i.e., Sertoli, Leydig, and myofibroblast cells) exhibited no staining affinity. EGF and EGFR were additionally detected in the epithelial lining of straight tubules and rete testis. Interestingly, the distribution of EGF and EGFR in the germ cells was mainly dependent upon the cycle of the seminiferous epithelium since their localization appeared to be preponderant during the spermatogonia proliferation and during the meiotic and spermiogenic processes. In conclusion, such findings may suggest that EGF and EGFR are important paracrine and/or autocrine regulators of spermatogenesis in bovine.

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References

  • Abd-Elmaksoud A, Sinowatz F (2005) Expression and localization of growth factors and their receptors in the mammalian testis. Review/Part I. Anat Histol Embryol 34(5):319–334

    Article  PubMed  CAS  Google Scholar 

  • Anklesaria P, Teixido J, Laiho M et al (1990) Cell–cell adhesion mediated by binding of membrane-anchored transforming growth factor alpha to epidermal growth factor receptors promotes cell proliferation. Proc Natl Acad Sci U S A 87(9):3289–3293

    Article  PubMed  CAS  Google Scholar 

  • Ascoli M (1981) Regulation of gonadotropin receptors and gonadotropin responses in a clonal strain of Leydig tumor cells by epidermal growth factor. J Biol Chem 256(1):179–183

    PubMed  CAS  Google Scholar 

  • Bartlett JM, Spiteri-Grech J, Nieschlag E (1990) Regulation of insulin-like growth factor I and stage-specific levels of epidermal growth factor in stage synchronized rat testes. Endocrinology 127(2):747–758

    PubMed  CAS  Google Scholar 

  • Carpenter G, Cohen S (1990) Epidermal growth factor. J Biol Chem 265(14):7709–7712

    PubMed  CAS  Google Scholar 

  • Caussanel V, Tabone E, Mauduit C et al (1996) Cellular distribution of EGF, TGFalpha and their receptor during postnatal development and spermatogenesis of the boar testis. Mol Cell Endocrinol 123(1):61–69

    Article  PubMed  CAS  Google Scholar 

  • Cupp AS, Skinner MK (2001) Expression, action, and regulation of transforming growth factor alpha and epidermal growth factor receptor during embryonic and perinatal rat testis development. J Androl 22(6):1019–1029

    PubMed  CAS  Google Scholar 

  • Fisher DA, Lakshmanan J (1990) Metabolism and effects of epidermal growth factor and related growth factors in mammals. Endocr Rev 11(3):418–442

    PubMed  CAS  Google Scholar 

  • Foresta C, Caretto A, Varotto A et al (1991) Epidermal growth factor receptors (EGFR) localization in human testis. Arch Androl 27(1):17–24

    PubMed  CAS  Google Scholar 

  • Foresta C, Varotto A (1994) Immunocytochemical localization of epidermal growth factor receptors in human testis from infertile subjects. Fertil Steril 61(5):941–948

    PubMed  CAS  Google Scholar 

  • Gnessi L, Fabbri A, Spera G (1997) Gonadal peptides as mediators of development and functional control of the testis: an integrated system with hormones and local environment. Endocr Rev 18:541–609

    Article  PubMed  CAS  Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    PubMed  CAS  Google Scholar 

  • Huleihel M, Lunenfeld E (2004) Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl 6:259–268

    PubMed  CAS  Google Scholar 

  • Kaloglu C, Bulut HE, Onarlioglu B (2000) Epidermal growth factor receptor (EGFR) immunolocalization in the male rat reproductive tract during pre- and postnatal periods. Turk J Vet Anim Sci 24:501–509

    Google Scholar 

  • Leblond CP, Clermont Y (1952) Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann NY Acad Sci 55:548–573

    Article  PubMed  CAS  Google Scholar 

  • Levine E, Cupp AS, Miyashiro L et al (2000) Role of transforming growth factor-alpha and the epidermal growth factor receptor in embryonic rat testis development. Biol Reprod 62(3):477–490

    Article  PubMed  CAS  Google Scholar 

  • Liu A, Flores C, Kinkead T et al (1994) Effects of sialoadenectomy and epidermal growth factor on testicular function of sexually mature male mice. J Urol 152(2 Pt 1):554–561

    PubMed  CAS  Google Scholar 

  • Massague J, Cheifetz S, Boyd FT et al (1990) TGF-beta receptors and TGF-beta binding proteoglycans: recent progress in identifying their functional properties. Ann NY Acad Sci 593:59–72

    Article  PubMed  CAS  Google Scholar 

  • Moore A, Morris ID (1993) Paracrine effects via the epidermal growth factor receptor in the rodent testis may be mediated by non-Leydig interstitial cells. J Endocrinol 136(3):439–446

    Article  PubMed  CAS  Google Scholar 

  • Mullaney BP, Skinner MK (1992) Transforming growth factor-alpha and epidermal growth factor receptor gene expression and action during pubertal development of the seminiferous tubule. Mol Endocrinol 6(12):2103–2113

    Article  PubMed  CAS  Google Scholar 

  • Nakazumi H, Sasano H, Maehara I et al (1996) Transforming growth factor-alpha, epidermal growth factor, and epidermal growth factor receptor in human testis obtained from biopsy and castration: immunohistochemical study. Tohoku J Exp Med 178(4):381–388

    Article  PubMed  CAS  Google Scholar 

  • Niederberger CS, Shubhada S, Kim SJ et al (1993) Paracrine factors and the regulation of spermatogenesis. World J Urol 11(2):120–128

    Article  PubMed  CAS  Google Scholar 

  • O”Rand MG, Romerell LG (1977) Appearance of the cell surface auto-and isoantigens during spermatogenesis in the rabbit. Dev Biol 55:347–358

    Article  CAS  Google Scholar 

  • Onyango DW, Wango EO, Otiang’a-Owiti GE et al (2000) Morphological characterization of the seminiferous cycle in the goat (Capra hircus): a histological and ultrastructural study. Anat Anz 182:235–241

    Article  CAS  Google Scholar 

  • Ortavant R (1958) Study of spermatogonial generations in the ram. C R Seances Soc Biol Fil 148:1958–1961

    Google Scholar 

  • Prigent SA, Lemoine NR (1992) The type 1 (EGFR-related) family of growth factor receptors and their ligands. Prog Growth Factor Res 4(1):1–24

    Article  PubMed  CAS  Google Scholar 

  • Radhakrishnan B, Oke BO, Papadopoulos V et al (1992) Characterization of epidermal growth factor in mouse testis. Endocrinology 131(6):3091–3099

    Article  PubMed  CAS  Google Scholar 

  • Radhakrishnan B, Suarez-Quian CA (1992) Characterization of epidermal growth factor receptor in testis, epididymis and vas deferens of non-human primates. J Reprod Fertil 96(1):13–23

    Article  PubMed  CAS  Google Scholar 

  • Russe I, Sinowatz F (1991) Lehrbuch der Embryologie der Haustiere. Verlag Paul Parey, Berlin und Hamburg

    Google Scholar 

  • Sharpe RM (1994) Regulation of spermatogenesis. In Knobil E, Neill JD (eds) The physiology of reproduction, 2nd edn. Raven Press, New York. pp 1363–1434

    Google Scholar 

  • Sordoillet C, Chauvin MA, de Peretti E et al (1991) Epidermal growth factor directly stimulates steroidogenesis in primary cultures of porcine Leydig cells: actions and sites of action. Endocrinology 128(4):2160–2168

    Article  PubMed  CAS  Google Scholar 

  • Stubbs SC, Hargreave TB, Habib FK (1990) Localization and characterization of epidermal growth factor receptors on human testicular tissue by biochemical and immunohistochemical techniques. J Endocrinol 125(3):485–492

    PubMed  CAS  Google Scholar 

  • Suarez-Quian CA, Dai MZ, Onoda M et al (1989) Epidermal growth factor receptor localization in the rat and monkey testes. Biol Reprod 41(5):921–932

    Article  PubMed  CAS  Google Scholar 

  • Suarez-Quian CA, Niklinski W (1990) Immunocytochemical localization of the epidermal growth factor receptor in mouse testis. Biol Reprod 43(6):1087–1097

    Article  PubMed  CAS  Google Scholar 

  • Suarez-Quian CA, Oke BO, Radhakrishnan B (1994) Relationship between submandibular gland epidermal growth factor and spermatogenesis in C3H mice. Tissue Cell 26(3):285–298

    Article  PubMed  CAS  Google Scholar 

  • Syed V, Khan SA, Nieschlag E (1991) Epidermal growth factor stimulates testosterone production of human Leydig cells in vitro. J Endocrinol Invest 14(2):93–97

    PubMed  CAS  Google Scholar 

  • Tsutsumi O, Kurachi H, Oka T (1986) A physiological role of epidermal growth factor in male reproductive function. Science 233(4767):975–977

    Article  PubMed  CAS  Google Scholar 

  • Uguralp S, Bay Karabulut A, Mizrak B et al (2004) The effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsion. Urol Res 32(5):323–331

    Article  PubMed  CAS  Google Scholar 

  • Verhoeven G, Cailleau J (1986) Stimulatory effects of epidermal growth factor on steroidogenesis in Leydig cells. Mol Cell Endocrinol 47(1–2):99–106

    Article  PubMed  CAS  Google Scholar 

  • Wahab-Wahlgren A, Martinelle N, Holst M et al (2003) EGF stimulates rat spermatogonial DNA synthesis in seminiferous tubule segments in vitro. Mol Cell Endocrinol 201(1–2):39–46

    Article  PubMed  CAS  Google Scholar 

  • Wong RW, Kwan RW, Mak PH et al (2000) Overexpression of epidermal growth factor induced hypospermatogenesis in transgenic mice. J Biol Chem 275(24):18297–18301

    Article  PubMed  CAS  Google Scholar 

  • Wrobel KH, Pawar HS (1992) Quantitative morphology of the testicular tubular epithelium in the water buffalo (Bubalus bubalis). Andrologia 24:63–68

    Article  PubMed  CAS  Google Scholar 

  • Wrobel KH, Reichold J, Schimmel M (1995) Quantitative morphology of the ovine seminiferous epithelium. Anat Anz 177:19–32

    CAS  Google Scholar 

  • Wrobel KH, Schimmel M (1989) Morphology of the bovine Sertoli cell during the spermatogenetic cycle. Cell Tissue Res 257:93–103

    Article  PubMed  CAS  Google Scholar 

  • Wu HH, Kawamata H, Kawai K et al (1993) Immunohistochemical localization of epidermal growth factor and transforming growth factor alpha in the male rat accessory sex organs. J Urol 150:990–993

    PubMed  CAS  Google Scholar 

  • Yan YC, Sun YP, Zhang ML (1998) Testis epidermal growth factor and spermatogenesis. Arch Androl 40(2):133–146

    PubMed  CAS  Google Scholar 

  • Yang GS, Lu RK, Chen ZD (2002) Clinical significance of EGF and EGFR expression changes in cryptorchid boys. Asian J Androl 4(4):255–258

    PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Anatomy and Histology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt

    M. Kassab & Mona A. Ali

  2. Department of Histology and Cytology, Faculty of Veterinary Medicine, Mansoura University, Gihan street, Mansoura, Egypt

    Ahmed Abd-Elmaksoud

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  1. M. Kassab
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  2. Ahmed Abd-Elmaksoud
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  3. Mona A. Ali
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Correspondence to Ahmed Abd-Elmaksoud.

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Kassab, M., Abd-Elmaksoud, A. & Ali, M.A. Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis. J Mol Hist 38, 207–214 (2007). https://doi.org/10.1007/s10735-007-9089-2

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  • DOI: https://doi.org/10.1007/s10735-007-9089-2

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