Abstract
Objective
Dental granulomas (DGs) and radicular cysts (RCs) are chronic periapical lesions frequently involving the jaws. Langerhans cells (LCs) are dendritic cells responsible for the presentation of antigens to T lymphocytes. This study examined the expression of LCs in DG and RCs by immunohistochemical staining.
Study Design
Eighteen cases of DGs and 26 cases of RCs were analyzed using anti-CD1a marker.
Results
CD1a-labeled LCs were observed in 11.1% of DGs and in 69.2% of RCs, showing a significant correlation (P < 0.0001; Fisher’s test). In DGs, LCs were only observed in granulation tissue, showing discrete immunostaining density. In RCs, LCs exhibited both a round and a dendritic shape in all epithelial layers. Although a correlation was observed between immunostaining density and epithelial thickness, as well as between immunostaining and inflammatory intensity, the differences were not significant in radicular cysts.
Conclusion
Langerhans cells provide important insight into the immunopathogenesis of chronic periapical lesions.
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References
Akhlaghi N, Dourov N (1995) Langerhans cells in odontogenic cysts. A retrospective study based on 142 cases. Bull Group Int Rech Sci Stomatol Odontol 38(3–4):71–76
Albuquerque RL Jr, Miguel RC, Costa AL et al (2003) Correlation of c-erbB-2 and S-100 expression with the malignancy grading and anatomical site in oral squamous cell carcinoma. Int J Exp Pathol 84(6):259–265
Babi LFS (1998) Las células de Langerhans en la inmunidad cutánea. Con especial referencia a la dermatitis atópica. Acta Dermatol 3:173–181
Barton G, Medzhitov R (2002) Control of adaptive immune responses by Toll-like receptors. Curr Opin Immunol 14(3):380–383
Cutler CW, Jotwani R (2004) Antigen-presentation and the role of dendritic cells in periodontitis. Periodontol 2000 35:135–57
Holíková Z, Hercogova J, Pizak J et al (2001) Dendritic cells in the skin and mucosa: what´s new. J Eur Acad Dermatol Venereol 15(2):116–120
Lombardi T, Hauser C, Budtz-Jorgensen E (1993) Langerhans cells: structure, function and role in oral pathological conditions. J Oral Pathol Med 22(5):193–202
Marton IJ, Kiss C (2000) Protective and destructive immune reactions in apical periodontitis. Oral Microbiol Immunol 15(3):139–150
Meghji S, Qureshi W, Henderson B et al (1996) The role of endotoxin and cytokines in the pathogenesis of odontogenic cysts. Arch Oral Biol 41(6):523–531
Moreira PR, Santos DF, Martins RD et al (2000) CD57+ cells in radicular cyst. Int Endod J 33(2):99–102
Peña-Cruz V, Ito S, Dascher CC et al (2003) Epidermal Langerhans cells efficiently mediate CD1a-dependent presentation of microbial lipid antigens to T cells. J Invest Dermatol 121(3):517–521
Piattelli A, Rubini C, Iezzi G et al (2002) CD1a-positive cells in odontogenic cysts. J Endod 28(4):267–268
Pringle GA, Daley TD, Veinot LA et al (1992) Langerhan´s cell histiocytosis in association with periapical granulomas and cyst. Oral Surg Oral Med Oral Pathol 74(2):186–192
Reynolds NJ, Yi JY, Fisher GJ et al (1995) Down-regulation of Langerhans cell protein kinase C-beta isoenzyme expression in inflammatory and hyperplastic dermatoses. Br J Dermatol 133(2):157–167
Santos JN (2000) Expressão imuno-histoquímica de citoqueratinas, CD1a, S-100, p53, p21, PCNA e Ki-67 em queilite actínica. Thesis, University of São Paulo, Brazil
Sequier S, Godeau G, Leborgne M et al (2000) Quantitative morphological analysis of Langerhans cells in health and diseased human gingival. Arch Oral Biol 45(12):1073–1081
Sequier S, Bodineau A, Godeau G et al (2003) Langerin+ versus CD1a+ Langerhans cells in human gingival tissue: a comparative and quantitative immunohistochemical study. Arch Oral Biol 48(4):255–262
Sjogren U, Mukohyama H, Roth C et al (2002) Bone-resorbing activity from cholesterol-exposed macrophages due to enhanced expression of interleukin-1alpha. J Dent Res 81(1):11–16
Sugihara A, Okamoto H, Horio T (2005) Effects of UVB on fascin expression in dendritic cells and Langerhans cells. J Dermatol Sci 40(3):177–185
Suzuki T, Kumamoto H, Ooya K et al (2001) Immunohistochemical analysis of CD1a-labeled Langerhans cells in human dental periapical inflammatory lesions – correlation with inflammatory cells and epithelial cells. Oral Dis 7(6):336–343
Tsai CH, Weng SF, Yang LC et al (2004) Immunohistochemical localization of tissue-type plasminogen activator and type I plaminogen activator inhibitor in radicular cysts. J Oral Pathol Med 33(3):156–161
Yamazaki M, Cheng J, Hao N et al (2004) Basement membrane-type heparan sulfate proteoglycan (perlecan) and low-density lipoprotein (LDL) are co-localized in granulation tissues: a possible pathogenesis of cholesterol granulomas in jaw cysts. J Oral Pathol Med 33(3):177–184
Acknowledgments
We thank Ana Carvalho, Mírian de Jesus Moraes, Maria de Lourdes dos Santos and Nancy Silva Santos for excellent technical assistance. This work was supported by FAPESB, Grants 1431040047950 and 200/04.
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Santos, L.C., Ramos, E.A.G., Gurgel, C.A.S. et al. Immunohistochemical detection of Langerhans cells in dental granulomas and radicular cysts. J Mol Hist 38, 201–205 (2007). https://doi.org/10.1007/s10735-007-9088-3
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DOI: https://doi.org/10.1007/s10735-007-9088-3