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Effects of 24-epibrassinolide on antioxidation defense and osmoregulation systems of young grapevines (V. vinifera L.) under chilling stress

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Abstract

The objective of this study was to investigate the influence of exogenous 24-epibrassinolide (EBR) on the substances involved in antioxidation and osmoregulation responses of young grape plants under chilling stress. The grapevine leaves were sprayed with 0 (control), 0.05, 0.10 or 0.15 mg/L of 24-epibrassinolide and then exposed to 4 and 0 °C for 24 h, respectively. The EBR treatment significantly enhanced the activities of antioxidative enzymes such as catalase, superoxide dismutase, peroxidase and ascorbate peroxidase in the plant leaves compared with the control. The contents of ascorbic acid and reduced glutathione increased after the EBR treatment, while reactive oxygen species (ROS) and lipid peroxidation were inhibited. In addition, the EBR treatment also greatly increased the contents of free proline, soluble protein, and soluble sugar. These results indicated that exogenous EBR treatment could enhance the antioxidation defense system and reduce oxidative damage caused by ROS and lipid oxidation in the young grapevine leaves. Meanwhile, it was found that the treatment could also increase the osmoregulation substance content in the grapevine leaves and improve their resistance against chilling stress.

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Abbreviations

BRs:

Brassinosteroids

EBR:

24-epibrassinolide

CAT:

Catalase

SOD:

Superoxide dismutase

POD:

Peroxidase

APX:

Ascorbate peroxidase

AsA:

Ascorbate

GSH:

Reduced glutathione

ROS:

Reactive oxygen species

H2O2 :

Hydrogen peroxide

OH− :

Hydroxyl radical

O2 − :

Superoxide radical

MDA:

Malondialdehyde

NBT:

Nitroblue tetrazolium

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

FW:

Fresh weight

References

  • Ahammed GJ, Yuan HL, Ogweno JO, Zhou YH, Xia XJ, Mao WH, Shi K, Yu JQ (2012) Brassinosteroid alleviates phenanthrene and pyrene phytotoxicity by increasing detoxification activity and photosynthesis in tomato. Chemosphere 86:546–555

    Article  PubMed  CAS  Google Scholar 

  • Ahmad P, Bhardwaj R, Tuteja N (2012) Plant signaling under abiotic stress environment. In: Ahmad P and Prasad MNV (Eds.) Environmental adaptations and stress tolerance of plants in the era of climate change, Science Business, Media, LLC, pp 297–323

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216

    Article  CAS  Google Scholar 

  • Bajguz A (2000) Effect of brassinosteroids on nucleic acid and protein content in cultured cells of Chlorella vulgaris. Plant Physiol Biochem 38:209–215

    Article  CAS  Google Scholar 

  • Bajguz A, Hayat S (2009) Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem 47:1–8

    Article  PubMed  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Burbulis N, Jonytienė V, Kuprienė R, Blinstrubienė A (2011) Changes in proline and soluble sugars content during cold acclimation of winter rapeseed shoots in vitro. J Food Agric Environ 9:371–374

    Google Scholar 

  • Cramer GR, Urano K, Delrot S, Pezzotti M, Shinozaki K (2011) Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biol 11:163–176

    Article  PubMed  Google Scholar 

  • Ding HD, Zhu XH, Zhu ZW, Yang SJ, Zha DS, Wu XX (2012) Amelioration of salt-induced oxidative stress in eggplant by application of 24-epibrassinolide. Biol Plantarum 56:767–770

    Article  CAS  Google Scholar 

  • Fadzillah NM, Gill V, Finch RP, Burdon RH (1996) Chilling, oxidative stress and antioxidant responses in shoot cultures of rice. Planta 199:552–556

    Article  CAS  Google Scholar 

  • Fariduddin Q, Yusuf M, Chalkoo S, Hayat S, Ahmad A (2011) 28-homobrassinolide improves growth and photosynthesis in Cucumis sativus L. through an enhanced antioxidant system in the presence of chilling stress. Photosynthetica 49:55–64

    Article  CAS  Google Scholar 

  • Gao JF (2006) Plant physiology experiment instruction. Higher Education Press, Beijing (in Chinese)

    Google Scholar 

  • Hasan SA, Hayat S, Ali B, Ahmad A (2008) 28-homobrassinolide protects chickpea (Cicer arietinum) from cadmium toxicity by stimulating antioxidants. Environ Pollut 151:60–66

    Article  PubMed  CAS  Google Scholar 

  • Hodges DM, Delong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

    Article  CAS  Google Scholar 

  • Hu WH, Wu Y, Zeng JZ, He L, Zeng QM (2010) Chill-induced inhibition of photosynthesis was alleviated by 24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery. Photosynthetica 48:537–544

    Article  CAS  Google Scholar 

  • Kang YY, Guo SR, Li J, Duan JJ (2008) Effects of 24-Epibrassinolide on antioxidant system in cucumber seedlings roots under hypoxia stress. Sci Agric Sinica 41:153–161 (in Chinese)

    CAS  Google Scholar 

  • Kang YY, Yang X, Guo SR, Zhang YY (2011) Effects of 24-Epibrassinolide on carbohydrate metabolism and enhancement of tolerance to root-zone hypoxia in cucumber (Cucumis sativus L.). Sci Agric Sinica 44:2495–2503 (in Chinese)

    CAS  Google Scholar 

  • Kanwar MK, Bhardwaj R, Arora P, Chowdhary SP, Sharma P, Kumar S (2012) Plant steroid hormones produced under Ni stress are involved in the regulation of metal uptake and oxidative stress in Brassica juncea L. Chemosphere 86:41–49

    Article  PubMed  CAS  Google Scholar 

  • Kuk YI, Shin JS, Burgos NR, Hwang TE, Han O, Cho BH, Jung S, Guh JO (2003) Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Sci 43:2109–2117

    Article  CAS  Google Scholar 

  • Liu YJ, Zhao ZG, Si J, Di CX, Han J, An LZ (2009) Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana. Plant Growth Regul 59:207–214

    Article  CAS  Google Scholar 

  • Liu YJ, Jiang HF, Zhao ZG, An LZ (2011) Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana. J Plant Physiol 168:853–862

    Article  PubMed  CAS  Google Scholar 

  • Maia JM, Costa de Macedo CE, Voigt EL, Freitas JBS, Silveira JAG (2010) Antioxidative enzymatic protection in leaves of two contrasting cowpea cultivars under salinity. Biol Plantarum 54:159–163

    Article  CAS  Google Scholar 

  • Mazorra LM, Holton N, Bishop GJ, Núñez M (2011) Heat shock response in tomato brassinosteroid mutants indicates that thermotolerance is independent of brassinosteroid homeostasis. Plant Physiol Biochem 49:1420–1428

    Article  PubMed  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Nakashita H, Yasuda M, Nitta T, Asami T, Fujioka S, Arai Y, Sekimata K, Takatsuto S, Yamaguchi I, Yoshida S (2003) Brassinosteroid functions in a broad range of disease resistance in tobacco and rice. J Plant 33:887–898

    Article  CAS  Google Scholar 

  • Oidaira H, Sano S, Koshiba T, Ushimaru T (2000) Enhancement of antioxidative enzyme activities in chilled rice seedlings. J Plant Physiol 156:811–813

    Article  CAS  Google Scholar 

  • Özdemir F, Bor M, Demiral T, Türkan I (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L) under salinity stress. Plant Growth Regul 42:203–211

    Article  Google Scholar 

  • Posmyk MM, Bailly C, Szafrańska K, Janas KM, Corbineau F (2005) Antioxidant enzymes and isoflavonoids in chilled soybean (Glycine max (L.) Merr.) seedlings. J Plant Physiol 162:403–412

    Article  PubMed  CAS  Google Scholar 

  • Prasad TK, Anderson MD, Martin BA, Stewart CR (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6:65–74

    PubMed  CAS  Google Scholar 

  • Qu T, Liu RF, Wang W, An LZ, Chen T, Liu GX, Zhao ZG (2011) Brassinosteroids regulate pectin methylesterase activity and AtPME41 expression in Arabidopsis under chilling stress. Cryobiology 63:111–117

    Article  PubMed  CAS  Google Scholar 

  • Thomas RL, Jen JJ, Morr CV (1981) Changes in soluble and bound peroxidase, IAA oxidase during tomato fruit development. J Food Sci 47:158–161

    Article  CAS  Google Scholar 

  • Vardhini BV, Rao SSR (2003) Amelioration of osmotic stress by brassinosteroids on seed germination and seedling growth of three varieties of sorghum. Plant Growth Regul 41:25–31

    Article  CAS  Google Scholar 

  • Wang LJ, Li SS (2006) Salicylic acid-induced heat or cold tolerance in relationto Ca2+ homeostasis and antioxidant systems in young grape plants. Plant Sci 170:685–694

    Article  CAS  Google Scholar 

  • Wang BK, Zeng GW (1993) Effect of epibrassinolide on the resistance of rice seedlings to chilling injury. J Plant Physiol Mol Biol 19:38–42

    CAS  Google Scholar 

  • Xia XJ, Wang YJ, Zhou YH, Tao Y, Mao WH, Shi K, Asami T, Chen ZX, Yu JQ (2009) Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in Cucumber. Plant Physiol 150:801–814

    Article  PubMed  CAS  Google Scholar 

  • Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514

    PubMed  CAS  Google Scholar 

  • Yuan GF, Jia CG, Li Z, Sun B, Zhang LP, Liu N, Wang QM (2010) Effect of brassinosteroids on drought resistance and abscisic acid concentration in tomato under water stress. Sci Hortic 126:103–108

    Article  CAS  Google Scholar 

  • Zhang Y, Li YX, Zheng LJ, Zhu YF, Zhang YH (2011) A new seedless grape variety of high quality and big berry-”Zicui”. J Hebei For Sci and Tech 3:107–108 (in Chinese)

    Google Scholar 

  • Zhao W, Xi ZM, Lin G, Tang JF (2011) Effect of selenium on physiological and biochemical indexes of Vitis vinifera cv. Cabernet Sauvignon leaves under water stress. J Fruit Sci 28:984–990 (in Chinese)

    Google Scholar 

  • Zhou BY, Guo ZF, Liu ZL (2005) Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress. Crop Sci 45:599–605

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Technology System for Grape Industry (CARS-30-zp-9), the Natural Science Foundation of Shaanxi Province (2011JM3004) and the Scientific Research Program of Northwest A&F University (QN2009059). Thanks for the Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest, Ministry of Agriculture of China. We also thank Dr. Zhimin Xu in the Department of Food Science, Louisiana State University Agricultural Center for the critical review of the manuscript.

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Authors and Affiliations

  1. College of Enology, Northwest A&F University, No. 22 Xinong Rd., Yangling, 712100, China

    Zhumei Xi, Zhizhen Wang, Yulin Fang, Zhuyun Hu, Yong Hu, Minmin Deng & Zhenwen Zhang

  2. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, 712100, China

    Zhumei Xi, Yulin Fang & Zhenwen Zhang

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  1. Zhumei Xi
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  2. Zhizhen Wang
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  3. Yulin Fang
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  4. Zhuyun Hu
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  5. Yong Hu
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  6. Minmin Deng
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  7. Zhenwen Zhang
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Correspondence to Zhenwen Zhang.

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All the authors contributed equally to this work.

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Xi, Z., Wang, Z., Fang, Y. et al. Effects of 24-epibrassinolide on antioxidation defense and osmoregulation systems of young grapevines (V. vinifera L.) under chilling stress. Plant Growth Regul 71, 57–65 (2013). https://doi.org/10.1007/s10725-013-9809-4

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  • DOI: https://doi.org/10.1007/s10725-013-9809-4

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