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Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.

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Abstract

A protocol for in vitro multiplication of caper (Capparis spinosa L. subsp. rupestris) from nodal segments collected from mature plants was developed. For shoot multiplication, one auxin (indol-3-butyric acid, IBA) and cytokinins of two different classes were used: the N6-substituted adenine derivatives 6-benzylamino purine (BAP), and the two synthetic phenylurea derivatives N-phenyl-N′-benzothiazol-6-ylurea (PBU) and N-phenyl-N′-(1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ). Maximum shoot production was achieved from explants cultured with the adeninic cytokinin BAP (4 μM) and the auxin IBA (0.5 μM). New shoots longer than 1 cm were used for rooting. To induce root formation, three auxins [indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 3-Indoleacetic acid (IAA)] and two synthetic phenylurea derivatives [N,N-bis-(2,3-methylenedioxyphenyl)urea (2,3-MDPU) and N,N-bis-(3,4-methylenedioxyphenyl)urea (3,4-MDPU)] were used. All rooting compounds tested stimulated the formation of roots. However, the best result in terms of a high percentage of rooted shoots having a well-developed root system with many lateral roots was achieved with the synthetic phenylurea 2,3-MDPU (1 μM) with 93.7% of well rooted plantlets. About 80% of rooted plantlets were successfully acclimatized and transferred to the greenhouse.

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Abbreviations

BAP:

6-benzylaminopurine

DMSO:

Dimethylsulfoxide

GRF:

Growth regulator free

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

2,3-MDPU:

N,N-bis-(2,3-methylenedioxyphenyl)urea

3,4-MDPU:

N,N-bis-(3,4-methylenedioxyphenyl)urea

NAA:

1-Naphthaleneacetic acid

PBU:

N-phenyl-N′-benzothiazol-6-ylurea

SEE:

Shoot elongation efficiency

TDZ:

Thidiazuron

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Correspondence to Francesco Carimi.

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Carra, A., Del Signore, M.B., Sottile, F. et al. Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.. Plant Growth Regul 66, 229–237 (2012). https://doi.org/10.1007/s10725-011-9645-3

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