Abstract
In our previous study, we have isolated novel HMW-GSs with unusually large molecular weight from Aegilops sharonensis; and to introduce this novel HMW-GSs variant into common wheat, we produced a number of amphiploids between various tetraploid wheat, hexaploid wheat and Ae. sharonensis by distant hybridization. In this study, we reported that the characterization of 24 HMW-GSs homozygous lines from wheat-Ae. sharonensis populations derived by continuous backcross or self-cross to common wheat cultivar LM3, which possessed homozygous HMW-GSs compositions including alien HMW-GS of Ae. sharonensis plus B and/or D genomes encoded HMW-GSs of LM3. According to the profiling of HMW-GS, these 24 homozygous lines can be summarized as four types. The results of Non-denaturing fluorescence in situ hybridization (ND-FISH) indicated that the chromosome numbers of eight of 24 lines were 40, 42, 44 or 45, and the number of alien Ae. sharonensis Ssh were between two and eight. Three 1S(1A) substitution lines, two 1S(1B) substitution lines, three 1S(1D) substitution lines. For the four lines with 44 and 45 chromosomes, they exhibit novel cytogenetic characteristics in which the substitution of 1S(1B) or 1S(1D) and addition of Ae. sharonensis 3S or 4S simultaneously appeared. The 24 homozygous progenies were similar to common wheat LM3 on agronomic traits. Specially, four lines of them showed almost identical agronomic traits to recurrent parent common wheat LM3 than the rest lines. These homozygous progenies could be used not only to evaluate function on quality of single HMW-GS but also to create translocation lines.
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References
Anderson OD, Greene FC (1989) The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor Appl Genet 77:689–700
Belton PS (1999) On the elasticity of wheat gluten. J Cereal Sci 29:103–107
Chen SY, Zhang AJ, Fu J (1991) The hybridization between Triticum aestivum and Psathyrostachys huashanica. Acta Genet Sin 18:508–512
Cui HF, Yu ZY, Deng JY, Gao X, Sun Y, Xia GM (2009) Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization. Planta 229:323–330
D’Ovidio R, Porceddu E, Lafiandra D (1994) PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-D1 locus. Theor Appl Genet 88:175–180
Feeney KWN, Gilbert SM, Halford NG, Tatham AS, Ps SPB (2003) Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length. Biopolymers 72:123–131
Forde J, Malpica JM, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Acids Res 13:6817–6832
Fu S, Chen L, Wang Y, Li M, Yang Z, Qiu L, Yan B, Ren Z, Tang Z (2015) Oligonucleotide probes for ND-FISH analysis to identify rye and wheat chromosomes. Sci Rep 5:10552
Gao X, Liu SW, Sun Q, Xia GM (2010) High frequency of HMW-GS sequence variation through somatic hybridization between Agropyron elongatum and common wheat. Planta 231:245–250
Gianibelli MC, Larroque OR, Macritchie F, Wrigley CW (2001) Biochemical, genetic, and molecular characterization of wheat endosperm proteins. Cereal Chem 78:635–646
Gupta RB, Paul JG, Cornish GB, Palmer GA, Bekes F, Rathjen AJ (1994) Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1, of common wheats. its additive and interaction effects on dough properties. J Cereal Sci 19:19–29
Halford NG, Field JM, Blair H, Urwin P, Moore K, Robert L, Thompson R, Flavell RB, Tatham AS, Shewry PR (1992) Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83:373–378
Han F, Lamb JC, Birchler JA (2006) High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proc Natl Acad Sci USA 103:3238–3243
Jiang QT, Wei YM, Wang F, Wang JR, Yan ZH, Zheng YL (2009) Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions. BMC Plant Biol 9:16
Jiang QT, Jian M, Wei YM, Liu YX, Lan XJ, Dai SF, Lu ZX, Shan Z, Zhao QZ, Zheng YL (2012) Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding. BMC Plant Biol 12:73
Jiang QT, Zhang XW, Ma J, Wei L, Zhao S, Zhao QZ, Qi PF, Lu ZX, Zheng YL, Wei YM (2014) Characterization of high-molecular-weight glutenin subunits from Eremopyrum bonaepartis and identification of a novel variant with unusual high molecular weight and altered cysteine residues. Planta 239:865–875
Kilian B, Mammen K, Millet E, Sharma R, Graner A, Salamini F, Hammer K, Özkan H (2011) Aegilops. In: Kole C (ed) Wild crop relatives: genomic and breeding resources. Springer, Berlin, pp 1–76
Lafiandra D, D’Ovidio R, Porceddu E, Margiotta B, Colaprico G (1993) New data supporting high mr glutenin subunit 5 as the determinant of quality differences among the pairs 5 + 10 vs. 2 + 12. J Cereal Sci 18:197–205
Lawrence GJ, Shepherd KW (1981) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31
Lee YK, Bekes F, Gras P, Ciaffi M, Morell MK, Appels R (1999) The low-molecular-weight glutenin subunit proteins of primitive wheats. IV. Functional properties of products from individual genes. Theor Appl Genet 98:149–155
Liu CY, Shepherd KW (1995) Inheritance of B subunits of glutenin and ω- and γ-gliadins in tetraploid wheats. Theor Appl Genet 90:1149–1157
Liu S, Zhao S, Chen F, Xia G (2007) Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum. BMC Evol Biol 7:76
Liu S, Zhao F, Gao X, Chen F, Xia G (2010) A novel high molecular weight glutenin subunit from Australopyrum retrofractum. Amino Acids 39:385–392
Mackie AM, Lagudah ES, Sharp PJ, Lafiandra D (1996) Molecular and biochemical characterisation of HMW glutenin subunits from T. tauschii and the D genome of hexaploid wheat. J Cereal Sci 23:213–225
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Acids Res 8:4321–4325
Olivera PD, Steffenson BJ (2009) Aegilops sharonensis: origin, genetics, diversity, and potential for wheat improvement. Botany 87:740–756
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Biol 38:141–153
Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin: Part 1: allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236
Payne PI, Holt LM, Hutchinson J, Bennett MD (1984) Development and characterisation of a line of bread wheat, Triticum aestivum, which lacks the short-arm satellite of chromosome 1B and the Gli-B1 locus. Theor Appl Genet 68:327–334
Payne PI, Holt LM, Krattiger AF, Carrillo JM (1988) Relationships between seed quality characteristics and HMW glutenin subunit composition determined using wheats grown in Spain. J Cereal Sci 7:229–235
Qi W, Yao T, Wei Z, Li D, Diao C, Xu L, Jian Z, Yi W, Xing F, Sha L (2016) Molecular cytogenetic characterization of a new wheat-rye 1BL·1RS translocation line expressing superior stripe rust resistance and enhanced grain yield. Planta 244:405–416
Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120
Shewry PR, Napier JA, Tatham AS (1995a) Seed storage proteins: structures and biosynthesis. Plant Cell 7:945–956
Shewry PR, Tatham AS, Barro F, Barcelo P, Lazzeri P (1995b) Biotechnology of breadmaking: unravelling and manipulating the multi-protein gluten complex. Nat Biotech 13:1185–1190
Sun X, Hu S, Liu X, Qian W, Hao S, Zhang A, Wang D (2006) Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit. Theor Appl Genet 113:631–641
Tang Z, Yang Z, Fu S (2014) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet 55:313–318
Tatham AS, Shewry PR (1995) The S-poor prolamins of wheat, barley and rye. J Cereal Sci 22:1–16
Van Slageren M (1994) Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Agricultural University Wageningen, Wageningen
Wang JR, Yan ZH, Wei YM, Zheng YL (2006) Characterization of high-molecular-weight glutenin subunit genes from Elytrigia elongata. Plant Breed 125:89–95
Wei L, Wang Q, Zhang LL, Ma J, Wang JR, Qi PF, Li W, Chen GY, Lan XJ, Deng M (2015) Genetic analyses of Glu-1S sh in wheat/Aegilops sharonensis hybrid progenies and development of alien HMW-GSs gene-specific markers. Mol Breeding 35:230
Yu G, Champouret N, Steuernagel B, Olivera PD, Simmons J, Williams C, Johnson R, Moscou MJ, Green P, Sela H (2017) Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis. Theor Appl Genet 130:1–16
Zhang R, Mingyi Z, Xiue W, Peidu C (2014) Introduction of chromosome segment carrying the seed storage protein genes from chromosome 1 V of Dasypyrum villosum showed positive effect on bread-making quality of common wheat. Appl Genet 127:523–533
Zhao QZ, Jiang QT, Yang Q, Ma J, Wang JR, Chen GY, Liu YX, Lu ZX, Wei YM, Zheng YL (2014) Characterization of intergeneric hybrid between common wheat and Aegilops sharonensis (Eig) and transfer of alien high molecular weight glutenin subunits into wheat. Cereal Res Commun 42:640–647
Acknowledgements
This work was supported by the National Key Research and Development Program of China (2017YFD0100900), the Key Research and Development Program of Sichuan Province (2018NZDZX0002), the Technological Innovation Project of Chengdu, Sichuan Province, China (2018-YF05-00059-SN), the Technology Foundation for Selected Overseas Chinese Scholars, Ministry of Personnel of China (No. 2014-4).
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JQT conceived and designed research. LXY conducted experiments and wrote the manuscript. WQ and LSY contributed cross in the fields. MJ, QPF, LW, and PZE contribute to investigation of agronomic traits, CGY and WJR contributed to development of molecular marker, TZX and YZH assisted on FISH, LXJ and DM analyzed data, LZX, WYM and ZYL revised the manuscript. All authors read and approved the manuscript.
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Li, X., Wang, Q., Li, S. et al. Stable expression and heredity of alien Glu-1Ssh in wheat-Aegilops sharonensis hybrid progenies. Genet Resour Crop Evol 66, 619–632 (2019). https://doi.org/10.1007/s10722-018-00736-8
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DOI: https://doi.org/10.1007/s10722-018-00736-8