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Genetic diversity and origin of cultivated potatoes based on plastid microsatellite polymorphism

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Abstract

The origin of cultivated potatoes has remaining questions. In this study, 237 accessions of all cultivated species and 155 accessions of wild species closely related to cultivated potatoes, including their putative ancestors, were analyzed using 15 plastid microsatellites (SSRs) to investigate genetic diversity and their relationships with the wild species. We here used polymorphic plastid SSRs we developed from potato plastid genome sequences as well as already known plastid SSR markers. All 15 loci were polymorphic and identified a total of 127 haplotypes. Dramatic decreases in levels of genetic diversity were revealed in landraces in comparison with wild ancestor species. The plastid SSR results showed a decrease in haplotype number and diversity from Peru to both north and south. Phylogenetic analysis revealed two distinct groups. One of them, group A, contained the majority of accessions of cultivated species of the Solanum tuberosum Andigenum group including all accessions of cultivated diploid and triploid cytotypes of this group (S. chaucha, S. phureja, and S. stenotomum by a former taxonomic system) and most of tetraploid accessions of the S. tuberosum Andigenum group (S. tuberosum subsp. andigenum), and the majority of accessions of wild ancestors from the northern members of the S. brevicaule complex. Another group B comprised most of the wild species accessions and almost exclusively hybrid cultivated species which have introgressed plastid genomes from the other wild gene pools. Lack of clustering of traditional cultivated species (as used above) support a revised group classification of S. tuberosum.

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Acknowledgments

This study was supported by International Science and Technology Center (ISTC), grant no. 3329, by the ARS/USDA (http://www.vir.nw.ru/biot), by RFBR grant no. 11-04-01002-a and by NSF DEB 0316614 entitled PBI Solanum: A worldwide treatment http://www.nhm.ac.uk/research-curation/research/projects/solanaceaesource/). We also acknowledge Dr. S. Kiru for providing germplasm from VIR potato collection.

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Authors and Affiliations

  1. N. I. Vavilov Institute of Plant Industry, Bolshaya Morskaya Street, 42-44, 190000, Saint Petersburg, Russia

    Tatjana Gavrilenko, Olga Antonova, Anna Shuvalova, Ekaterina Krylova, Natalia Alpatyeva & Lubov Novikova

  2. Saint Petersburg State University, Saint Petersburg, Russia

    Tatjana Gavrilenko

  3. USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI, USA

    David M. Spooner

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  1. Tatjana Gavrilenko
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  2. Olga Antonova
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  3. Anna Shuvalova
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  4. Ekaterina Krylova
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  5. Natalia Alpatyeva
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  6. David M. Spooner
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  7. Lubov Novikova
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Correspondence to Tatjana Gavrilenko.

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Gavrilenko, T., Antonova, O., Shuvalova, A. et al. Genetic diversity and origin of cultivated potatoes based on plastid microsatellite polymorphism. Genet Resour Crop Evol 60, 1997–2015 (2013). https://doi.org/10.1007/s10722-013-9968-1

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