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The Frankincense tree (Boswellia sacra, Burseraceae) from Oman: ITS and ISSR analyses of genetic diversity and implications for conservation

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Abstract

DNA sequences from the ITS region of the nuclear genome and Inter-Simple Sequence Repeat markers (ISSR) were used to estimate genetic diversity among and within populations of the Frankincense tree Boswellia sacra from Dhofar, Oman. This is a culturally and ecologically relevant species that is showing symptoms of decline due to anthropic factors and, possibly, global warming. ITS sequences were 511 bp long and showed low (6.4%) variation among geographically different populations. The four selected ISSR primers yielded 93 reproducible bands, of which 91 (97.9%) were polymorphic in the 97 individual profiles obtained. Total genetic diversity (H T) and average heterozygosity within populations (H S) resulted fairly low (0.22 and 0.136, respectively). The accession from Wadi Dowkah, an UNESCO world heritage site, showed the lowest level of genetic diversity (H E = 0.107), while the eastern populations from the Hasik area harboured a slightly greater amount of variation. Analysis of Molecular Variance showed that differentiation among populations was relatively high (38.1%), possibly due to the reduced gene flow between the largely isolated stands of Boswellia (N m = 0.39). Genetic distances and AMOVA suggested a clear differentiation between the eastern and western coastal populations, while those from the internal area did not form a consistent group. For conservation, the eastern sites should be given priority as core populations harbouring significant amounts of allelic diversity. Reasons for reinforcing the more depauperated stands, such as Wadi Dowkah, with local plant material only or, alternatively, with the introduction of germplasm from genetically distinct stands are discussed.

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References

  • Avanzini A (2000) Il porto di Sumharam nel Dhufâr. In: De Maigret A (ed) Yemen, nel paese della Regina di Saba. Skira, Milano, pp 231–233

    Google Scholar 

  • Becerra JX, Venable DL (1999) Nuclear ribosomal DNA phylogeny and its implications for evolutionary trends in Mexican Bursera (Burseraceae). Am J Bot 86:1047–1057

    Article  CAS  PubMed  Google Scholar 

  • Bhat KV, Babrekar PP, Lakhanpaul S (1999) Study of genetic diversity in Indian and exotic sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers. Euphytica 110:21–33

    Article  CAS  Google Scholar 

  • Borchsenius F (2007) FastGap 1.0.8. Software distributed by the author (http://192.38.46.42/aubot/fb/FastGap_home.htm)

  • Bornet B, Branchard M (2001) Nonanchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19:209–215

    Article  CAS  Google Scholar 

  • Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acid Res 16:10881–10890

    Article  CAS  PubMed  Google Scholar 

  • El Quassani AS (1984) Dhofar the land of Frankincense. International Printing Press, Ruwi

    Google Scholar 

  • Excoffier L, Smouse PE, Quattro M (1992) Analysis of molecular variance inferred from metric distances among DNA Haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Feyissa T, Nybom H, Bartish IV, Welander M (2007) Analysis of genetic diversity in the endangered tropical tree species Hagenia abyssinica using ISSR markers. Genet Resour Crop Evol 54:947–958

    Article  Google Scholar 

  • Fleishman E, Launer AE, Switky KR, Yandell U, Heywood J, Murphy DD (2001) Rules and exceptions in conservation genetics: genetic assessment of the endangered plant Cordylanthus palmatus and its implications for management planning. Biol Conserv 98:45–53

    Article  Google Scholar 

  • Frankel OH, Brown AHD, Burdon JJ (1995) The conservation of plant biodiversity. Cambridge University Press, Cambridge

    Google Scholar 

  • Ge XJ, Yu Y, Yuan Y, Huang H, Yan C (2005a) Genetic diversity and geographic differentiation in endangered Ammopiptanthus (Leguminosae) populations in desert regions of northwest China as revealed by ISSR analysis. Ann Bot 95:843–851

    Article  CAS  PubMed  Google Scholar 

  • Ge XJ, Zhang LB, Yuan YM, Hao G, Chiang TY (2005b) Strong genetic differentiation of the East-Himalayan Megacodon stylophorus (Gentianaceae) detected by inter-simple sequence repeats (ISSR). Biodivers Conserv 14:849–861

    Article  Google Scholar 

  • Ghazanfar SA (1994) Handbook of Arabian medicinal plants. CRC Press, Boca Raton

    Google Scholar 

  • Groom N (2000) I profumi d’Arabia. In: De Maigret A (ed) Yemen, nel paese della Regina di Saba. Skira, Milano, pp 87–89

    Google Scholar 

  • Hammer K, Gebauer J, Al Khanjari S, Buerkert A (2009) Oman at the cross-roads of inter-regional exchange of cultivated plants. Genet Resour Crop Evol 56:547–560

    Article  Google Scholar 

  • Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding and genetic resources. Sinauer, Sunderland MA, pp 43–63

    Google Scholar 

  • Hatcher PE, Wilkinson MJ, Albani MC, Hebbern CA (2004) Conserving marginal populations of the food plant (Impatiens noli-tangere) of an endangered moth (Eustroma reticulatum) in a changing climate. Biol Conserv 116:305–317

    Article  Google Scholar 

  • Hedrén M (2004) Species delimitation and the partitioning of genetic diversity – an example from the Carex flava complex (Cyperaceae). Biodivers Conserv 13:293–316

    Article  Google Scholar 

  • Holsinger KE, Gottlieb LK (1991) Conservation of rare and endangered plants: principle and prospect. In: Falk DA, Holsinger KE (eds) Genetics and conservation of rare plants. Oxford University Press, New York, pp 195–298

    Google Scholar 

  • Huenneke LF (1991) Ecological implications of genetic variations in plant populations. In: Falk DA, Holsinger KE (eds) Genetics and conservation of rare plants. Oxford University Press, New York, pp 31–44

    Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  • Li J, Jin Z (2007) Genetic variation and differentiation in Torreya jackii Chun, an endangered plant endemic to China. Plant Sci 172:1048–1053

    Article  CAS  Google Scholar 

  • Lowe A, Harris S, Ashton P (2004) Ecological genetics. Design, analysis, and application. Blackwell, Oxford

    Google Scholar 

  • Luo X, Zhuang X, Yang Y (2007) Genetic diversity of Camellia changii Ye (Theaceae) using ISSR markers. J Trop Subtrop Bot 15(2):93–100

    CAS  Google Scholar 

  • Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220

    CAS  PubMed  Google Scholar 

  • Meloni M, Perini D, Filigheddu R, Binelli G (2006) Genetic variation in five mediterranean populations of Juniperus phoenicea as revealed by inter-simple sequence repeat (ISSR) markers. Ann Bot 97:299–304

    Article  CAS  PubMed  Google Scholar 

  • Mengoni A, Selvi F, Cusimano N, Galardi F, Gonnelli C (2006) Genetic diversity inferred from AFLP fingerprinting in populations of Onosma echioides (Boraginaceae) from serpentine and calcareous soils. Plant Biosyst 140:211–782

    Article  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    CAS  PubMed  Google Scholar 

  • Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155

    Article  CAS  PubMed  Google Scholar 

  • Nybom H, Bartish I (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspect Plant Ecol Evol Syst 3(2):93–114

    Article  Google Scholar 

  • Okun DO, Kenya EU, Oballa PO, Odee DW, Muluvi GM (2008) Analysis of genetic diversity in Eucalyptus grandis (Hill ex Maiden) seed sources using inter simple sequence repeats (ISSR) molecular markers. Afr J Biotechnol 7(13):2119–2123

    CAS  Google Scholar 

  • Pérez-Collazos E, Catalán P (2007) Genetic diversity analysis and conservation implications for the Iberian threatened populations of the irano-turanian relict Krascheninnikovia ceratoides (Chenopodiaceae). Biol J Linn Soc 92:419–429

    Article  Google Scholar 

  • Raffaelli M, Tardelli M (2007) L’incenso tra mito e realtà. Produzione, commercio e raccolta della resina, ieri e oggi. Le specie di Boswellia (Burseraceae) conosciute. Conservazione delle piante in natura. Centro Studi Erbario Tropicale, Università di Firenze. Pubbl. no. 108, Firenze

  • Raffaelli M, Mosti S, Tardelli M (2003a) The Frankincense Tree (Boswellia sacra Flueck., Burseraceae) in Dhofar, southern Oman: field-investigations on the natural populations. Webbia 58(1):133–149

    Google Scholar 

  • Raffaelli M, Tardelli M, Mosti S (2003b) The Wadi Doka Frankincense Park in Dhofar, Oman – first steps towards the safeguard and improvement of Boswellia sacra (Burseraceae). Pubbl. Erbario Tropicale di Firenze no. 93, Firenze

  • Raffaelli M, Mosti M, Tardelli M (2006) Boswellia sacra Flueck. (Burseraceae) in the Hasik area (eastern Dhofar, Oman) and a list of the surrounding flora. Webbia 61(2):245–251

    Google Scholar 

  • Raffaelli M, Tardelli M, Mosti S (2008) Preserving and restoring the frankincense tree (Boswellia sacra) at Wadi Doka: a work in progress. In Avanzini A. (Ed.), A port in Arabia between Rome and the Indian Ocean (3rd C. BC–5th C. AD), Khor Rori Report 2. “L’Erma” di Breitschneider. Roma, pp 715–723

  • Rakoczy-Trojanowska M, Bolibok H (2004) Characteristics and comparison of three classes of microsatellite-based markers and their application in plants. Cell Mol Biol Lett 9:221–238

    CAS  PubMed  Google Scholar 

  • Reddy MP, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

    Article  Google Scholar 

  • Rees AR (1995) Frankincense and Myrrh. New Plantsman 2(1):55–59

    Google Scholar 

  • Richards CM (2000) Inbreeding depression and genetic rescue in a plant metapopulation. Am Nat 155:383–827

    Article  PubMed  Google Scholar 

  • Rieseberg LH (1996) Homology among RAPD fragments in interspecific comparisons. Mol Ecol 5:99–105

    Article  CAS  Google Scholar 

  • Rohlf FJ (1990) NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 2.02. Exeter, New York

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Schneider S, Rosselli D, Excoffier L (2000) Arlequin: a software for population genetics data analysis. Version 2.000. University of Geneva

  • Sica M, Gamba G, Montieri S, Gaudio L, Aceto S (2005) ISSR markers show differentiation among Italian populations of Asparagus acutifolius L. BMC Genet 6:17

    Article  PubMed  Google Scholar 

  • Simmons MP, Ochoterena H (2000) Gaps as characters in sequence-based phylogenetic analyses. Syst Biol 49:369–381

    Article  CAS  PubMed  Google Scholar 

  • Slatkin M (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139:457–462

    CAS  PubMed  Google Scholar 

  • Swofford DL (1998) PAUP*. Phylogenetic analysis using parsimony (and other methods) vers. 4.0. Sinauer, Sunderland

    Google Scholar 

  • Thulin M, Warfa AM (1987) The Frankincense trees (Boswellia ssp., Burseaceae) of Northern Somalia and Southern Arabia. Kew Bull 42(3):487–500

    Article  Google Scholar 

  • Weising K, Nybom H, Wolf K, Kahl G (2005) DNA fingerprinting in plants: principles, methods and applications. CRC Press, Boca Raton

    Book  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Shinsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Xia T, Chen S, Chen S, Zhang D, Zhang D, Gao Q, Ge X (2007) ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic Rhodiola chrysanthemifolia (Crassulaceae). Biochem Syst Ecol 35:209–221

    Article  CAS  Google Scholar 

  • Xiao M, Li Q, Wang L, Guo L, Li J, Tang L, Chen F (2006) ISSR analysis of the genetic diversity of the endangered species Sinopodophyllum hexandrum (Royle) Ying from Western Sichuan Province, China. J Integr Plant Biol 48(10):1140–1146

    Article  CAS  Google Scholar 

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Acknowledgments

The authors wish to acknowedge the Office for Cultural Affair of Sultanate of Oman for the logistic support in Dhofar. Dr. Stefano Mosti and sig. Marcello Tardelli helped with field sampling and Dr. Alessio Mengoni gave useful insights into the technical aspects of data analysis. An anonymous reviewer suggested useful comments on the first version of the manuscript. Research funds from M.I.U.R. 40% to M. Raffaelli are acknowledged.

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Authors and Affiliations

  1. Department of Plant Biology, University of Florence, via La Pira 4, 50121, Florence, Italy

    Andrea Coppi, Lorenzo Cecchi, Federico Selvi & Mauro Raffaelli

  2. Centro Studi Erbario Tropicale, via La Pira 4, 50121, Florence, Italy

    Federico Selvi & Mauro Raffaelli

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  1. Andrea Coppi
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  2. Lorenzo Cecchi
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  4. Mauro Raffaelli
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Correspondence to Andrea Coppi.

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Coppi, A., Cecchi, L., Selvi, F. et al. The Frankincense tree (Boswellia sacra, Burseraceae) from Oman: ITS and ISSR analyses of genetic diversity and implications for conservation. Genet Resour Crop Evol 57, 1041–1052 (2010). https://doi.org/10.1007/s10722-010-9546-8

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