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Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry

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Abstract

Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine. Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their possible structural characteristics in CCA are not clear. In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography. The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths. The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.

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Abbreviations

AMAC:

2-aminoacridone

CCA :

Colla corii asini

CS:

chondroitin sulfate

DS:

dermatan sulfate

EIC:

extracted ion chromatogram

GAG:

glycosaminoglycan

GalNAc:

N-acetylgalatosamine

GlcA:

glucuronate

GlcNAc:

N-acetylglucosmine

HA:

hyaluronic acid

HP:

heparin

HS:

heparan sulfate

IdoA:

iduronate

LC-MS-ITTOF:

liquid chromatography-ion trap/time-of-flight mass spectrometry

NaCl:

sodium chloride

ΔUA:

4,5-unsaturated uronate (generated by chondroitinase excision)

SE-HPLC:

size-exclusion HPLC

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC 21343013).

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Correspondence to Quntao Liang or Zheng Wei.

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Huang, H., Liu, S., Du, J. et al. Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry. Glycoconj J 37, 201–207 (2020). https://doi.org/10.1007/s10719-019-09904-z

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