Skip to main content

Advertisement

SpringerLink
Log in

Enhanced fucosylation of GA1 in the digestive tracts of X-ray-irradiated mice

  • Original Article
  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

In mice at 4 days after X-ray-irradiation at 0.5 Gy/min for 16 min, the tissue weights of immune organs, i.e., thymus and spleen, were decreased due to injury to lymphocytes by the X-rays. The resulting immunosuppressive condition allowed the growth of lactobacilli, i.e., L. murinus, which contained LacβTH-DG and possessed the ability to induce transcription of the fucosyltransferase gene for synthesis of FGA1. LacβTH-DG was detected in the jejunal and ileal contents of X-ray-irradiated mice, but not in those of control mice, whereas LacTetH-DG of L. johnsonii was present in the stomach and caecal contents of both mice. The amounts of FGA1 in the duodenal and jejunal tissues of X-ray-irradiated mice increased to 4- and 9-fold of those in controls, respectively. Reflecting the enhanced fucosylation of GA1, the total amounts of FGA1 excreted into the contents of X-ray-irradiated mice were 1.4-times higher than those in controls. Also, when the extent of enhanced fucosylation of GA1 in several regions of the digestive tracts of X-ray-irradiated mice was compared with that in immune deficient nude, scid and pIgR(−/−) mice, the more than 4-fold increases of FGA1 observed in duodenal and jejunal tissues corresponded to those in pIgR(−/−) mice without secretory IgA. Since an increased amount of FGA1 in the small intestine was observed only 4 days after X-ray-irradiation, and diminished synthesis of FGA1 occurred on administration of penicillin and streptomycin, fucosylation of GA1 in the small intestine was revealed to occur quickly in response to a change in the intestinal bacterial population.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

ᅟ:

The nomenclature for glycolipids is based on the recommendations of the IUPAC-IUB Commission on Biochemical Nomenclature [1]

Fs:

Forssman antigen

GA1:

asialo GM1

FGA1:

fucosyl GA1

FGM1:

fucosyl GM1

DH-DG:

dihexaosyl diglycerides

TH-DG:

trihexaosyl diglycerides

TetH-DG:

tetrahexaosyl diglycerides

LacDH-DG:

Lactobacillus DH-DG, Galα1-2Glcα1–3’DG

LacTH-DG:

Galα1-6Galα1-2Glcα1–3’DG

LacβTH-DG:

Glcβ1-6Galα1-2Glcα1–3’DG

LacTetH-DG:

Galα1-6Galα1-6Galα1-2Glcα1–3’DG

Sta:

Saphylococcus

Str:

Streptococcus

MRS:

deMan, Rogosa and Sharpe

PBS:

phosphate-buffered saline

References

  1. IUPAC-IUB: Commission on Biochemical Nomenclature. Eur. J. Biochem. 179, 11–21 (1977)

    Google Scholar 

  2. Suzuki, A., Yamakawa, T.: The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. J. Biochem. 90, 1541–1544 (1981)

    Article  CAS  PubMed  Google Scholar 

  3. Iwamori, M., Shibagaki, T., Nakata, Y., Adachi, S., Nomura, T.: Distribution of receptor glycolipids for lactobacilli in murine digestive tract and production of antibodies cross-reactive with them by immunization of rabbits with lactobacilli. J. Biochem. 146, 185–191 (2009)

    Article  CAS  PubMed  Google Scholar 

  4. Iwamori, M., Iwamori, Y., Adachi, S., Nomura, T.: Excretion into feces of asialo GM1 in the murine digestive tract and lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria. Glycoconj. J. 28, 21–30 (2011)

    CAS  PubMed  Google Scholar 

  5. Iwamori, M., Sakai, A., Minamimoto, N., Iwamori, Y., Tanaka, K., Aoki, D., Adachi, S., Nomura, T.: Characterization of novel glycolipid antigens with an α-galactose epitope in lactobacilli detected with rabbit anti-lactobacillus antisera and occurrence of antibodies against them in human sera. J. Biochem. 150, 515–523 (2011)

    Article  CAS  PubMed  Google Scholar 

  6. Iwamori, M., Nakasa, M., Yamazaki, K., Iwamori, Y., Tanaka, K., Aoki, D., Adachi, S., Nomura, T.: Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic lactobacillus. Staphylococcus and Streptococcus species. Glycoconj. J. 29, 199–209 (2012)

    Article  CAS  PubMed  Google Scholar 

  7. Iwamori, M., Iwamori, Y., Matsumoto, S., Adachi, S., Nomura, T.: Enhanced expression of fucosyl GA1 in the digestive tract of immune-deficient scid, nude and pIgR(−/−) mice. J. Biochem. 154, 541–549 (2013)

    Article  CAS  PubMed  Google Scholar 

  8. Iwamori, M., Iwamori, Y., Adachi, S., Nomura, T.: Changes in bacterial glycolipids as an index of intestinal lactobacilli and epithelial glycolipids in the digestive tracts of mice after administration of penicillin and streptomycin. Glycoconj. J. 30, 889–897 (2013)

    Article  CAS  PubMed  Google Scholar 

  9. Iwamori, M., Tanaka, K., Adachi, S., Aoki, D., Nomura, T.: Absence of lactobacilli containing glycolipids with the α-galactose epitope and the enhanced fucosylation of a receptor glycolipid GA1 in the digestive tracts of immune-deficient scid mice. J. Biochem. 158, 73–82 (2015)

    Article  CAS  PubMed  Google Scholar 

  10. Karlsson, K.A.: Animal glycosphingolipids as membrane attachment sites for bacteria. Annu. Rev. Biochem. 58, 309–350 (1989)

    Article  CAS  PubMed  Google Scholar 

  11. Yamamoto, K., Miwa, T., Taniguchi, H., Nagano, T., Shimamura, K., Tanaka, T., Kumagai, H.: Binding specificity of lactobacillus to glycolipids. Biochem. Biophys. Res. Commun. 228, 148–152 (1996)

    Article  CAS  PubMed  Google Scholar 

  12. Neeser, J.R., Granato, D., Rouvet, M., Servin, A., Teneberg, S., Karlsson, K.A.: Lactobacillus johnsonii La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria. Glycobiology. 10, 1193–1199 (2000)

    Article  CAS  PubMed  Google Scholar 

  13. Umesaki, Y., Setoyama, H., Matsumoto, S., Imaoka, A., Itoh, K.: Differential roles of segmented filamentous bacteria and clostridia in development of the intestinal immune system. Infect. Immun. 67, 3504–3511 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Bry, L., Falk, P.G., Midtvedt, T., Gordon, J.I.: A model of host-microbial interactions in an open mammalian ecosystem. Science. 273, 1380–1383 (1996)

    Article  CAS  PubMed  Google Scholar 

  15. Iwamori, M., Kaido, T., Iwamori, Y., Ohta, Y., Tsukamoto, K., Kozaki, S.: Involvement of the C-terminal tail of Arthrobacter ureafaciens sialidase isoenzyme M in cleavage of the internal sialic acid of ganglioside GM1. J. Biochem. 138, 327–334 (2005)

    Article  CAS  PubMed  Google Scholar 

  16. Iwamori, M., Ohta, Y., Uchida, Y., Tsukada, Y.: Arthrobacter ureafaciens sialidase isoenzymes, L, M1 and M2, cleave fucosyl GM1. Glycoconj. J. 14, 67–73 (1997)

    Article  CAS  PubMed  Google Scholar 

  17. Lin, B., Hayashi, Y., Saito, M., Sakakibara, Y., Yanagisawa, M., Iwamori, M.: GDP-fucose: beta-galactoside α1,2-fucosyltransferase, MFUT-II, and not MFUT-I or -III, is induced in a restricted region of the digestive tract of germ-free mice by host-microbe interactions and cycloheximide. Biochim. Biophys. Acta. 1487, 275–285 (2000)

    Article  CAS  PubMed  Google Scholar 

  18. Iwamori, M., Domino, S.E.: Tissue-specific loss of fucosylated glycolipids in mice with targeted deletion of α1,2-fucosyltransferase genes. Biochem. J. 380, 75–81 (2004)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Lin, B., Saito, M., Sakakibara, Y., Hayashi, Y., Yanagisawa, M., Iwamori, M.: Characterization of three members of murine α1,2-fucosyltransferases: change in the expression of the Se gene in the intestine of mice after administration of microbes. Arch. Biochem. Biophys. 388, 207–215 (2001)

    Article  CAS  PubMed  Google Scholar 

  20. Domino, S.E., Zhang, L., Lowe, J.B.: Molecular cloning, genomic mapping, and expression of two secretor blood group α1,2-fucosyltransferase genes differentially regulated in mouse uterine epithelium and gastrointestinal tract. J. Biol. Chem. 276, 23748–23756 (2001)

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan

    Masao Iwamori

  2. Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Kyoko Tanaka & Daisuke Aoki

  3. Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Osaka, Ibaraki, 567-0085, Japan

    Shigeki Adachi & Taisei Nomura

Authors
  1. Masao Iwamori
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyoko Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shigeki Adachi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daisuke Aoki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Taisei Nomura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masao Iwamori.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Iwamori, M., Tanaka, K., Adachi, S. et al. Enhanced fucosylation of GA1 in the digestive tracts of X-ray-irradiated mice. Glycoconj J 34, 163–169 (2017). https://doi.org/10.1007/s10719-016-9746-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10719-016-9746-3

Keywords

Search

Navigation