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Changes in bacterial glycolipids as an index of intestinal lactobacilli and epithelial glycolipids in the digestive tracts of mice after administration of penicillin and streptomycin

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Abstract

The major lipid constituent of symbiotic gram-positive bacteria in animals are phosphatidylglycerol, cardiolipin and dihexaosyl diglycerides (DH-DG), whose hydrophobic structures are characteristic of the environments, and the carbohydrate structures of DH-DGs are bacterial species-characteristic. Immunization of rabbits with intestinal lactobacilli generated antibodies against DH-DGs and their modified structures, among which Galα1-6-substituted DH-DG, i.e., Lactobacillus tetrahexaosyl diglyceride (LacTetH-DG), reacted with antibodies more intensely than DH-DG. Whereas, from the 16S-rRNA sequence, the intestinal lactobacilli in murine digestive tracts were revealed to be L. johnsonii, in which LacTetH-DG is present at the concentration of 2.2 ng per 1 × 106 cells. To obtain more accurate estimates of intestinal lactobacilli in several regions of the digestive tract of mice, LacTetH-DG was detected by TLC-immunostaining with anti-Lactobacillus antisera, being found in the stomach, cecum and colon of normal breeding mice, 1.0 × 109, 3.5 × 109 and 7.4 × 109 cells, respectively. Administration of penicillin and streptomycin for 6 days resulted in a reduction in the number of intestinal lactobacilli, the levels being 0 %, 30 % and 4 % of the control ones in the stomach, cecum and colon, respectively, which was associated with the accumulation of the contents in the tracts from the stomach to the cecum and with diarrhea. In addition, a reduced amount of fucosyl GA1 (FGA1) and a compensatory increase in GA1 due to the reduced activity of α1,2-fucosyltransferase in the small intestine and the enhanced discharge of FGA1 into the contents occurred in mice, probably due to the altered population of bacteria caused by administration of penicillin and streptomycin.

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Abbreviations

PS:

Penicillin and streptomycin

GA1:

Asialo GM1

FGA1:

Fucosyl GA1

cy:

Cyclopropane

ai:

Anteiso

PG:

Phosphatidylglycerol

CL:

Cardiolipin

GL:

Glycolipid

LJ:

Lactobacillus johnsonii

LI:

Lactobacillus intestinalis

SE:

Staphylococcus epidermidis

LacDH-DG:

Lactobacillus dihexaosyl diglycerides

LacTH-DG:

Lactobacillus trihexaosyl diglycerides

LacTetH-DG:

Lactobacillus tetrahexaosyl diglycerides.

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Acknowledgement

This work was supported by JSPS KAKENHI Grant Numbers 24570141, 23592465 and 25462610.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan

    Masao Iwamori & Yuriko Iwamori

  2. Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan

    Shigeki Adachi & Taisei Nomura

Authors
  1. Masao Iwamori
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  2. Yuriko Iwamori
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  3. Shigeki Adachi
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  4. Taisei Nomura
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Correspondence to Masao Iwamori.

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Iwamori, M., Iwamori, Y., Adachi, S. et al. Changes in bacterial glycolipids as an index of intestinal lactobacilli and epithelial glycolipids in the digestive tracts of mice after administration of penicillin and streptomycin. Glycoconj J 30, 889–897 (2013). https://doi.org/10.1007/s10719-013-9494-6

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  • DOI: https://doi.org/10.1007/s10719-013-9494-6

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