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Neutral N-glycan patterns of the gastropods Limax maximus, Cepaea hortensis, Planorbarius corneus, Arianta arbustorum and Achatina fulica

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Abstract

The N-glycosylation potentials of Limax maximus, Cepaea hortensis, Planorbarius corneus, Arianta arbustorum and Achatina fulica were analysed by investigation of the N-glycan structures of the skin and viscera glycoproteins by a combination of HPLC and mass-spectrometry methods. It is one of the first steps to enlarge the knowledge on the glycosylation abilities of gastropods, which may help to establish new cell culture systems, to uncover new means for pest control for some species, and to identify carbohydrate-epitopes which may be relevant for immune response. All snails analysed contained mainly oligomannosidic and small paucimannosidic structures, often terminated with 3-O-methylated mannoses. The truncated structures carried modifications by β1-2-linked xylose to the β-mannose residue, and/or an α-fucosylation, mainly α1,6-linked to the innermost N-acetylglucosaminyl residue of the core. Many of these structures were missing the terminal N-acetylglucosamine, which has been shown to be a prerequisite for processing to complex N-glycans in the Golgi. In some species (Planorbarius corneus and Achatina fulica) traces of large structures, terminated by 3-O-methylated galactoses and carrying xylose and/or fucose residues, were also detected. In Planorbarius viscera low amounts of terminal α1-2-fucosylation were determined. Combining these results, gastropods seem to be capable to produce all kinds of structures ranging from those typical in mammals through to structures similar to those found in plants, insects or nematodes. The detailed knowledge of this very complex glycosylation system of the gastropods will be a valuable tool to understand the principle rules of glycosylation in all organisms.

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Abbreviations

Endoglycosidase H:

endo-β-N-acetylglucosaminidase H (E.C. 3.2.1.96)

peptide: N glycanase A:

peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase (E.C. 3.5.1.52)

HRP:

horseradish peroxidase

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Acknowledgements

This project was partly financed by the Austrian Fonds zur wissenschaftlichen Forschung Project number P13928-BIO. We want to thank Dr. Manfred Pintar (Department for Integrative Biology, Institute for Zoology, University of Natural Resources and Applied Life Sciences, Vienna,) for identification and classification of the snails and Dr. Iain Wilson for reading the manuscript. The technical help of Thomas Dalik, and Denise Kerner is highly appreciated.

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  1. Department of Chemistry, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria

    Martin Gutternigg, Sabine Bürgmayr, Gerald Pöltl, Judith Rudolf & Erika Staudacher

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  1. Martin Gutternigg
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Gutternigg, M., Bürgmayr, S., Pöltl, G. et al. Neutral N-glycan patterns of the gastropods Limax maximus, Cepaea hortensis, Planorbarius corneus, Arianta arbustorum and Achatina fulica . Glycoconj J 24, 475–489 (2007). https://doi.org/10.1007/s10719-007-9040-5

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