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Repetitive DNA sequences include retrotransposons in genomes of the Glomeromycota

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Abstract

Twenty-five repetitive elements are first described in the genomes of the arbuscular mycorrhizal (AM) fungi Gigaspora margarita, Gig. rosea and Glomus mosseae. Nineteen repetitive DNA sequences isolated by genomic library screening and four by self-priming PCR had no homology to known DNA sequences, except for two Gig. margarita sequences and one Gig. rosea sequence which showed amino acid similarity to retrotransposons. Part of the Gig. rosea sequence was also similar to a DNA transposon. Two other retrotransposon sequences were isolated using PCR targeting of reverse transcriptase and ribonuclease H domains. Evidence is provided for three gypsy-like LTR retrotransposon and two non-LTR retrotransposon sequences in the AM fungal genomes. Four contain stop codons indicating that they cannot be active. Expression of three retrotransposons was not detected in germinating spores or intraradical hyphae of Gig. margarita. Southern blot analyses indicated that these three sequences are dispersed in the genome and that two are methylated. Sequence analysis of different GmarLTR1 copies showed they have undergone mutations by transitions, which may have been induced by cytosine methylation. Transposable elements may have played a major role in shaping genome structure and size during evolution of the Glomeromycota.

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Abbreviations

AM fungus:

Arbuscular mycorrhizal fungus

EDTA:

Ethylenediaminetetraacetic acid

EN:

Endonuclease

GSP-PCR:

Genomic self-priming polymerase chain reaction

INT:

Integrase

LSU:

Large subunit

LTR:

Long terminal repeat

M-MLV:

Moloney murine leukemia virus

rDNA:

Ribosomal DNA

RNase H:

Ribonuclease H

RT:

Reverse transcriptase

SDS:

Sodium dodecyl sulfate

SSC:

Sodium salt citrate

SSU:

Small subunit

TAE:

Tris/acetic acid/EDTA

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Authors and Affiliations

  1. UMR 1088 INRA/5184 CNRS/Université de Bourgogne Plante-Microbe-Environnement, INRA-CMSE, BP 86510, 21065, Dijon Cedex, France

    Armelle Gollotte, Floriane L’Haridon, Odile Chatagnier, Guillaume Wettstein, Christine Arnould, Diederik van Tuinen & Vivienne Gianinazzi-Pearson

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  1. Armelle Gollotte
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  2. Floriane L’Haridon
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  3. Odile Chatagnier
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  4. Guillaume Wettstein
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  5. Christine Arnould
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  6. Diederik van Tuinen
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  7. Vivienne Gianinazzi-Pearson
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Correspondence to Armelle Gollotte.

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Gollotte, A., L’Haridon, F., Chatagnier, O. et al. Repetitive DNA sequences include retrotransposons in genomes of the Glomeromycota. Genetica 128, 455–469 (2006). https://doi.org/10.1007/s10709-006-0019-0

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