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Diversity of endogenous small non-coding RNAs in Oryza sativa

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Abstract

Small non-coding RNAs play important roles in regulating cell functions by controlling mRNA turnover and translational repression in eukaryotic cells. Here we isolated 162 endogenous small RNA molecules from Oryza sativa, which ranged from 16 to 35 nt in length. Further analysis indicated that they represented a diversity of small RNA molecules, including 17 microRNAs (miRNAs), 30 tiny non-coding RNAs (tncRNAs) and 20 repeat-associated small interfering RNAs (rasiRNAs). Among 17 miRNAs, 13 were novel miRNA candidates and their potential targets were important regulatory genes in the rice genome. We also found that a cluster of small RNAs, including many rasiRNAs, matched to a nuclear DNA fragment that evolutionarily derived from chloroplast. These results demonstrate clearly the existence of distinct types of small RNAs in rice and further suggest that small RNAs may control gene regulation through diverse mechanisms.

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  1. Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, 430072, Wuhan, P. R. China

    Zuyu Chen, Jingjing Zhang, Jin Kong, Shaoqing Li, Yan Fu, Shaobo Li, Hong Zhang, Yangsheng Li & Yingguo Zhu

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  1. Zuyu Chen
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  2. Jingjing Zhang
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  3. Jin Kong
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  4. Shaoqing Li
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  5. Yan Fu
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  7. Hong Zhang
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  8. Yangsheng Li
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  9. Yingguo Zhu
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Correspondence to Yingguo Zhu.

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Chen, Z., Zhang, J., Kong, J. et al. Diversity of endogenous small non-coding RNAs in Oryza sativa . Genetica 128, 21–31 (2006). https://doi.org/10.1007/s10709-005-2486-0

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