Abstract
This study evaluated the use of essential oil of Ocimum gratissimum (EOOG) for anesthesia and in transport of Colossoma macropomum. Experiment 1, Test 1, anesthesia induction and recovery times were determined using different EOOG concentrations (0, 20, 50, 100, 200, 300 mg L−1), with two size classes: Juveniles I (0.86 g) and Juveniles II (11.46 g) (independent tests in a completely randomized design). Based on the results of Test 1, in Test 2 Juveniles II were exposed to EOOG concentrations: 0, 20, 100 mg L−1. Tissue samples were collected immediately after induction and 1 h post-recovery, to assess oxidative status variables. Experiment 2, Juveniles I (0.91 g) and Juveniles II (14.76 g) were submitted to transport in water with different concentrations of EOOG (0, 5, 10 mg L−1) (independent tests in a completely randomized design). The effects on oxidative status variables were evaluated. Concentrations between 50 and 200 mg L−1 EOOG can be indicated for Juveniles I, while concentrations between 50 and 100 mg L−1 EOOG for Juveniles II. The concentration of 100 mg L−1 EOOG was able to prevent oxidative damage in the liver. In Experiment 2, the concentrations of 5 and 10 mg L−1 EOOG added to the transport water caused sedation for both studied size classes of juveniles and did not cause oscillations in water quality variables nor any mortality. The concentration of 10 mg L−1 EOOG improved the oxidative status. It can be concluded that EOOG can be used for anesthesia and transport of C. macropomum.
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References
Adewale AY, Adeshina I, Yusuf OY (2017) Anaesthetic effect of Ocimum gratissimum extract on Oreochromis niloticus juveniles. Eur Exp Biol 7:2–7
Araújo-Dairiki TB, Chaves FCM, Dairiki JK (2018) Seeds of sacha inchi (Plukenetia volubilis, Euphorbiaceae) as a feed ingredient for juvenile tambaqui, Colossoma macropomum, and matrinxã, Brycon amazonicus (Characidae). Acta Amaz 48:32–37. https://doi.org/10.1590/1809-4392201700753
Araújo-Lima CARM, Gomes L (2019) Tambaqui Colossoma macropomum. In: Baldisseroto B, Gomes L (eds) Espécies nativas para piscicultura no Brasil, 3ª. UFSM, Santa Maria, pp 147–168
Alvarenga CMD, Volpato GL (1995) Agonistic profile and metabolism in fry of the Nile tilapia. Physiol Behav 57:75–80. https://doi.org/10.1016/0031-9384(94)00206-K
Araújo-Lima CARM, Goulding M (1998) So fruitful fish: ecology, conservation, and aquaculture of the Amazon’s tambaqui. Columbia University Press, New York
Aydın B, Barbas LAL (2020) Sedative and anesthetic properties of essential oils and their active compounds in fish: A review. Aquaculture 520:734999. https://doi.org/10.1016/j.aquaculture.2020.734999
Barbas LAL, Maltez LC, Stringhetta GR, Garcia OL, Monserrat JM, Silva DT, Heinzmann BM, Sampaio LA (2017) Properties of two plant extractives as anaesthetics and antioxidants for juvenile tambaqui Colossoma macropomum. Aquaculture 469:79–87. https://doi.org/10.1016/j.aquaculture.2016.12.012
Barbas LAL, Araújo ERL, Torres MF, Maltez LC, Oliveira GL, Heinzmann BM, Sampaio LA (2020) Stress relieving potential of two plant-based sedatives in the transport of juvenile tambaqui Colossoma macropomum. Aquaculture 520:734681. https://doi.org/10.1016/j.aquaculture.2019.734681
Becker AG, Luz RK, Mattioli CC, Nakayama CL, Silva WS, Leme FDOP, Baldisserotto B (2017) Can the essential oil of Aloysia triphylla have anesthetic effect and improve the physiological parameters of the carnivorous freshwater catfish Lophiosilurus alexandri after transport? Aquaculture 481:184–190. https://doi.org/10.1016/j.aquaculture.2017.09.007
Becker AJ, Fogliarini CDO, Souza CDF, Becker AG, Mourão RHV, Silva LVFD, Baldisserotto B (2018) Ventilatory frequency and anesthetic efficacy in silver catfish, Rhamdia quelen: a comparative approach between different essential oils. R Bras Zootec 47:e20170185. https://doi.org/10.1590/rbz4720170185
Benovit SC, Gressler LT, Silva LL, Garcia OL, Okamoto MH, Pedron JS, Heinzmann BM, Baldisserotto B (2012) Anesthesia and transport of Brazilian flounder, Paralichthys orbignyanus, with essential oils of Aloysia gratissima and Ocimum gratissimum. J World Aquac Soc 43:896–900. https://doi.org/10.1111/j.1749-7345.2012.00604.x
Berka R (1986) The Transport of Live Fish. A Review, 48th ed. Eifac Technical Paper-FAO. Rome, Italy
Beutler E (1984) Superoxide dismutase. In: Beutler E (ed) Red cell metabolism. A manual of biochemical methods. Grune and Stratton, Philadelphia, pp 83–85
Boaventura TP, Souza CF, Ferreira AL, Favero GC, Baldissera MD, Heinzmann BM, Baldisserotto B, Luz RK (2020) Essential oil of Ocimum gratissimum (Linnaeus, 1753) as anesthetic for Lophiosilurus alexandri: induction, recovery, hematology, biochemistry and oxidative stress. Aquaculture 529:735676. https://doi.org/10.1016/j.aquaculture.2020.735676
Boaventura TP, Souza CF, Ferreira AL, Favero GC, Baldissera MD, Heinzmann BM, Luz RK (2021) The use of Ocimum gratissimum L. essential oil during the transport of Lophiosilurus alexandri: water quality, hematology, blood biochemistry and oxidative stress. Aquaculture 531:735964. https://doi.org/10.1016/j.aquaculture.2020.735964
Boaventura TP, Pedras PPC, Júlio GSC, Santos FAC, Ferreira AL, Silva WS, Luz RK (2022) Use of eugenol, benzocaine or salt during the transport of panga, Pangasianodon hypophthalmus (Sauvage, 1878): Effects on water quality, haematology and blood biochemistry. Aquac Res 53:1395–1403. https://doi.org/10.1111/are.15672
Boijink CL, Queiroz CA, Chagas EC, Chaves FCM, Inoue LAKA (2016) Anesthetic and anthelminthic effects of clove basil (Ocimum gratissimum) essential oil for tambaqui (Colossoma macropomum). Aquaculture 457:24–28. https://doi.org/10.1016/j.aquaculture.2016.02.010
Brandão FR, Melo SDC, Sebastino FA, Chaves FMC, Bizzo RH, O’Sullivan FAL, Chagas EC (2022) Essential oils as anaesthetics and sedatives in native Brazilian fish, with a special emphasis on Colossoma macropomum: A review. Aquac Res 53:767–781. https://doi.org/10.1111/are.15650
Bui TM, Phuong NT, Nguyen GH, Silva SS (2013) Fry and fingerling transportation in the striped catfish, Pangasianodon hypophthalmus, farming sector, Mekong Delta, Vietnam: a pivotal link in the production chain. Aquaculture 388–391:70–75. https://doi.org/10.1016/j.aquaculture.2013.01.007
Chagas EC, Brandão FR, Farias CFS, Monteiro PC, Batista E, Souza MDC, Inoue LAKA (2021) Alterações fisiológicas em juvenis de tambaqui transportados em solução de óleo essencial de Ocimum gratissimum. Agrarian 14:102–111. https://doi.org/10.30612/0621/10.30612/agrarian.v14i51.12279
Cheeseman KH, Slater TF (1994) An introduction to free radicals biochemistry. Br Med Bull 31:31–2692. https://doi.org/10.5860/choice.31-2692
CONCEA (2018) Diretriz da Prática de Eutanásia do Conselho Nacional de Controle de Experimentação Animal – CONCEA
Evelson P, Travacio M, Repetto M, Escobar J, Llesuy S, Lissi EA (2001) Evaluation of total reactive antioxidant potential (TRAP) of tissue homogenates and their cytosols. Arch Biochem Biophys 388:261–266. https://doi.org/10.1006/abbi.2001.2292
Favero GC, Silva WDS, Boaventura TP, Leme FDOP, Luz RK (2019) Eugenol or salt to mitigate stress during the transport of juvenile Lophiosilurus alexandri, a Neotropical carnivorous freshwater catfish. Aquaculture 512:734321. https://doi.org/10.1016/j.aquaculture.2019.734321
Ferreira AL, Silva WS, Neves CL, Ferreira NS, Takata R, Luz RK (2020) Benzocaine and menthol as anesthetics for the African cichlid Aulonocara nyassae. Aquac Int 28:1837–1846. https://doi.org/10.1007/s10499-020-00561-w
Ferreira AL, Favero GC, Boaventura TP, Freitas SC, Ferreira NS, Descovi SN, Baldisserotto B, Heinzmann BM, Luz RK (2021) Essential oil of Ocimum gratissimum (Linnaeus, 1753): efficacy for anesthesia and transport of Oreochromis niloticus. Fish Physiol Biochem 47:135–152. https://doi.org/10.1007/s10695-020-00900-x
Ferreira AL, Santos FAC, Souza AS, Favero GC, Baldisserotto B, Pinheiro CG, Pinheiro CG, Heinzmann BM, Luz RK (2022) Efficacy of Hesperozygis ringens essential oil as an anesthetic and for sedation of juvenile tambaqui (Colossoma macropomum) during simulated transport. Aquac Int 30:1549–1561. https://doi.org/10.1007/s10499-022-00868-w
Frova C (2006) Glutathione transferases in the genomics era: new insights and perspectives. Biomol Eng 23:149–169. https://doi.org/10.1016/j.bioeng.2006.05.020
Golombieski JI, Silva LVF, Baldisserotto B, Silva JHS (2003) Transport of silver catfish (Rhamdia quelen) fingerlings at different times, load densities, and temperatures. Aquaculture 216:95–102. https://doi.org/10.1016/S0044-8486(02)00256-9
Hermes-Lima M (2004) Oxygen in biology and biochemistry: role of free radicals. Functional metabolism: regulation and adaptation, pp. 319–366.
Hohlenwerger JC, Baldisserotto B, Couto RD, Heinzmann BM, Silva DTD, Caron BO, Copatti CE (2017) Essential oil of Lippia alba in the transport of Nile tilapia. Cienc Rural 47:e20160040. https://doi.org/10.1590/0103-8478cr20160040
Joseph PD, Mannervik B, Montellano PO (1997) Molecular Toxicology. Oxford University Press, New York, pp 152–186
Keene JI, Noakes DIG, Moccia RD, Soto GC (1998) The efficacy of clove oil as an anesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquac Res 29:89–101. https://doi.org/10.1046/j.1365-2109.1998.00927.x
Kehrer JP (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol 23:21–48. https://doi.org/10.3109/10408449309104073
Li ZH, Xie S, Wang JX, Sales J, Li P, Chen DQ (2009) Effect of intermittent starvation on growth and some antioxidant indexes of Macrobrachium nipponense (De Haan). Aquac Res 40:526–532. https://doi.org/10.1111/j.1365-2109.2008.02123.x
Morais IDS, O’sullivan FDA (2017) Biology, habitat and farming of tambaqui Colossoma macropomum (CUVIER, 1816). Sci Amazon 6:81–93
Neves LC, Silva WS, Ferreira AL, Favero GC, Beier SL, Palheta GDA, Melo NFAC, Luz RK (2022) Physiological responses of juvenile Colossoma macropomum after different periods of air exposure. Aquaculture 548:e737583. https://doi.org/10.1016/j.aquaculture.2021.737583
Nico L, Neilson M (2020) Colossoma macropomum (Cuvier, 1816): Nonindigenous Aquatic Species Database. Nonindigenous Aquatic Species Database, U.S. Geological Survey, Gainesville, FL
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358. https://doi.org/10.1016/0003-2697(79)90738-3
Okey IB, Igiri MR, Ekpenyong JJ, Inya FU (2022) Haematological and Biochemical responses in African catfish (Clarias gariepinus) juveniles immobilized with clove basil (Ocimum gratissimum) powder anaesthetic. J Aquat Sci Oceanogr 3:1–20
Paranhos CO, Neves LDC, Silva WS, Luz RK (2021) Transport of killifish Hypsolebias flagellatus: effects of salt use and previous feeding in association with transport time. J Appl Aquac 35:100–111. https://doi.org/10.1080/10454438.2021.1943102
Penna MAH, Villacorta-Corrêa MA, Walter T, Petrere Junior M (2005) Growth of the tambaqui Colossoma macropomum (Cuvier) (Characiformes: Characidae): which is the best model? Braz J Biol 65:129–139. https://doi.org/10.1590/S1519-69842005000100017
Poli G, Leonarduzzi G, Biasi F, Chiarpotto E (2012) Oxidative stress and cell signalling. Curr Med Chem 11:1163–1182. https://doi.org/10.2174/0929867043365323
Prabhu KS, Lobo R, Shirwaikar AA, Shirwaikar A (2009) Ocimum gratissimum: A review of its chemical, pharmacological and ethnomedicinal properties. J Tradit Complement Med 1:1–15. https://doi.org/10.2174/1876391X00901010001
Purbosari N, Warsiki E, Syamsu K, Santoso J (2019) Natural versus synthetic anesthetic for transport of live fish: A review. Aquac Fish 4:129–133. https://doi.org/10.1016/j.aaf.2019.03.002
Read SM, Northcote DH (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem 116:53–64. https://doi.org/10.1016/0003-2697(81)90321-3
Readman GD, Owen SF, Knowles TG, Murrell JC (2017) Species specific anaesthetics for fish anaesthesia and euthanasia. Sci Rep 7:1–7. https://doi.org/10.1038/s41598-017-06917-2
Ribeiro PAP, Miranda Filho KC, Melo DCD, Luz RK (2015) Efficiency of eugenol as anesthetic for the early lifestages of Nile tilapia (Oreochromis niloticus). An Acad Bras Cienc 87:529–535. https://doi.org/10.1590/00013765201520140024
Ribeiro AS, Batista EDS, Dairiki JK, Chaves FCM, Inoue LAKA (2016) Anesthetic properties of Ocimum gratissimum essential oil for juvenile matrinxã. Acta Sci Anim Sci 38:1–7. https://doi.org/10.4025/actascianimsci.v38i1.28787
Ribeiro PAP, Melo HDC, Oliveira CG, Della FMAL, Luz RK (2019) Eugenol and benzocaine as anesthetics for Lophiosilurus alexandri juvenile, a freshwater carnivorous catfish. Aquac Int 27:313–321. https://doi.org/10.1007/s10499-018-0326-3
Ross LG, Ross B (2008) Anaesthetic and sedative techniques for aquatic animals. Blackwell Science, Oxford
Sampaio FDF, Freire CA (2016) An overview of stress physiology of fish transport: changes in water quality as a function of transport duration. Fish Fish 17:1055–1072. https://doi.org/10.1111/faf.12158
Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205
Sena AC, Teixeira RR, Ferreira EL, Heinzmann BM, Baldisserotto B, Caron BO, Copatti CE (2016) Essential oil from Lippia alba has anaesthetic activity and is effective in reducing handling and transport stress in tambacu (Piaractus mesopotamicus×Colossoma macropomum). Aquaculture 465:374–379. https://doi.org/10.1016/j.aquaculture.2016.09.033
Silva CAD, Fujimoto RY (2015) Tambaqui growth in response to stocking density in cages. Acta Amaz 45:323–332. https://doi.org/10.1590/1809-4392201402205
Silva LL, Parodi TV, Reckziegel P, Oliveira GV, Bürger ME, Baldisserotto B, Pereira AMS, Heinzmann BM (2012) Essential oil of Ocimum gratissimum L.: Anesthetic effects, mechanism of action and tolerance in silver catfish. Rhamdia Quelen Aquaculture 350:91–97. https://doi.org/10.1016/j.aquaculture.2012.04.012
Silva LDL, Garlet QI, Koakoski G, Abreu MSD, Mallmann CA, Baldisserotto B, Barcellos LJG, Heinzmann BM (2015) Anesthetic activity of the essential oil of Ocimum americanum in Rhamdia quelen (Quoy & Gaimard, 1824) and its effects on stress parameters. Neotrop Ichthyol 13:715–722. https://doi.org/10.1590/1982-0224-20150012
Silva HNPD, Carvalho BCFD, Maia JLDS, Becker AG, Baldisserotto B, Heinzmann BM, Silva LVFD (2019) Anesthetic potential of the essential oils of Lippia alba and Lippia origanoides in tambaqui juveniles. Cienc Rural 49:e20181059. https://doi.org/10.1590/0103-8478cr20181059
Silva HNPD, Souza RND, Sousa EMDO, Mourão RHV, Baldisserotto B, Silva LVFD (2020) Citral chemotype of the Lippia alba essential oil as an additive in simulated transport with different loading densities of tambaqui juveniles. Cienc Rural 50:e20190815. https://doi.org/10.1590/0103-8478cr20190815
Small BC (2003) Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus. Aquaculture 218:177–185. https://doi.org/10.1016/S0044-8486(02)00302-2
Souza CF, Baldissera MD, Bianchini AE, Silva EG, Mourão RHV, Silva LVF, Baldisserotto B (2018) Citral and linalool chemotypes of Lippia alba essential oil as anesthetics for fish: a detailed physiological analysis of side effects during anesthetic recovery in silver catfish (Rhamdia quelen). Fish Physiol Biochem 44:21–34. https://doi.org/10.1007/s10695-017-0410-z
Souza CDF, Baldissera MD, Baldisserotto B, Heinzmann BM, Martos-Sitcha JA, Mancera JM (2019) Essential oils as stress-reducing agents for fish aquaculture: a review. Front Physiol 10:785–802. https://doi.org/10.3389/fphys.2019.00785
Storey KB (1996) Oxidative stress: animal adaptations in nature. Braz J Med Biol Res 29:1715–1733
Tarkhani R, Imani A, Jamali H, Farsani HG (2017) Anaesthetic efficacy of eugenol on various size classes of angelfish (Pterophyllum scalare Schultze, 1823). Aquac Res 48:5263–5270. https://doi.org/10.1111/are.13339
Velisek J, Stara A, Li ZH, Silovska S, Turek J (2011) Comparison of the effects of four anaesthetics on blood biochemical profiles and oxidative stress biomarkers in rainbow trout. Aquaculture 310:369–375. https://doi.org/10.1016/j.aquaculture.2010.11.010
Zhang X, Shan P, Sasidhar M, Chupp GL, Flavell RA, Choi AM, Lee PJ (2003) Reactive oxygen species and extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase mediate hyperoxia-induced cell death in lung epithelium. Am J Respir Cell Mol Biol 28:305–315. https://doi.org/10.1165/rcmb.2002-0156OC
Funding
This research was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brasil – 150950/2022–5; 402952/2021–9; 308547/2018–7; 301225/2017–6); Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG-Brasil – APQ-01531–21); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brasil – finance code 001); INCT ADAPTA II (Funded by CNPq 465540/2014–7); and Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM-Brasil – 062.1187/2017).
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Andre Lima Ferreira: Conceptualization, Methodology, Validation, Formal analysis, Resources, Investigation, Writing—Original Draft, Writing- Reviewing and Editing, Visualization, Funding Acquisition. Walisson de Souza e Silva: Conceptualization, Methodology, Validation, Formal analysis, Investigation. Hugo Napoleão Pereira da Silva: Conceptualization, Methodology, Validation, Formal analysis, Investigation. Carine de Freitas Milarch: Conceptualization, Methodology, Validation, Formal analysis, Investigation. Glauber David Almeida Palheta: Conceptualization, Methodology, Validation, Formal analysis, Investigation. Carlos Garrido Pinheiro: Methodology, Validation, Investigation. Berta Maria Heinzmann: Conceptualization, Methodology, Validation, Investigation, Resources, Writing—Reviewing and Editing. Bernardo Baldisserotto: Conceptualization, Methodology, Validation, Investigation, Formal analysis, Resources, Writing—Reviewing and Editing. Gisele Cristina Favero: Conceptualization, Methodology, Validation, Investigation, Resources, Writing—Reviewing and Editing. Ronald Kennedy Luz: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Resources, Data curation, Investigation, Writing—Original Draft, Writing—Reviewing and Editing, Visualization, Supervision, Project Administration, Funding Acquisition.
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All experimental methodologies were approved (under registration numbers 13/2021 and 14/2021) by the Ethics Committee on Animal Use (CEUA) of the Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil. The experiments were carried out independently at the Aquaculture Laboratory (LAQUA-UFMG).
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Highlights
• This is first study on the oxidative responses of juvenile of Colossoma macropomum exposed to essential oil of Ocimum gratissimum L. (EOOG).• EOOG was efficient for anesthesia of juvenile C. macropomum and improved the protection by the oxidative defense system after anesthesia and biometric handling.• Simulated transportation with 5 and 10 mg L−1 EOOG induced sedation of juvenile C. macropomum.
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Ferreira, A.L., de Souza e Silva, W., da Silva, H.N.P. et al. Oxidative responses in small juveniles of Colossoma macropomum anesthetized and sedated with Ocimum gratissimum L. essential oil. Fish Physiol Biochem (2024). https://doi.org/10.1007/s10695-024-01350-5
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DOI: https://doi.org/10.1007/s10695-024-01350-5